| DW Civils & Rail Ltd | Construction | 2013 |
| DWJ World Ltd | Health & social care | 2025 |
| Dxsure Ltd | Information & communication | 2024 |
| Dycut Building Services Ltd | Construction | 2018 |
| Dyeus Enterprise Limited | Agriculture & fishing | 2017 |
| Dylan Holdings Limited | Real estate | 2021 |
| Dylan Imports Limited | Wholesale & retail | 2004 |
| Dynamic Chapters Ltd | Manufacturing | 2025 |
| Dynamic Drylining Ltd | Construction | 2017 |
| Dynamic Exterior Cleaning Ltd | Administrative & support | 2025 |
| Dynamic Lift Services Ltd | Manufacturing | 2013 |
| Dynamic Network Solutions Ltd | Information & communication | 2015 |
| Dynamic Property Holdings Limited | Real estate | 2020 |
| Dynotex Limited | Administrative & support | 2026 |
| E & I Property Company Ltd | Real estate | 2020 |
| E & I Property Company WD FH Ltd | Real estate | 2024 |
| E Buzi Ltd | Real estate | 2023 |
| E Cigarettes Ltd | Wholesale & retail | 2025 |
| E D L Interior Specialists & Services Ltd | Construction | 2013 |
| E Kandola Ltd | Professional & scientific | 2025 |
| E&G Management Limited | Real estate | 2022 |
| E&R Transport Ltd | Transport & logistics | 2025 |
| E'Reigns Ltd | Wholesale & retail | 2021 |
| E'S Events Limited | Hospitality | 2019 |
| E-Quints Consulting Limited | Information & communication | 2016 |
| E-Quints Medicare Ltd | Administrative & support | 2019 |
| E.B.G. (UK) Limited | Wholesale & retail | 1993 |
| E.C.S Contractors Ltd | Construction | 2014 |
| E.T Village Ltd | Wholesale & retail | 2023 |
| E1 Consulting Ltd | Professional & scientific | 2021 |
| E1 Properties Ltd | Real estate | 2019 |
| EA Electrical Services Kent Ltd | Construction | 2026 |
| Eadie Group Ltd | Wholesale & retail | 2026 |
| Eagle EYE Building Ltd | Construction | 2023 |
| Eagle Plumbing & Heating Ltd | Construction | 2011 |
| Eagle Team Ltd | Construction | 2014 |
| EAGLE84 Constructions Ltd | Construction | 2021 |
| EAO Ventures Ltd | Real estate | 2023 |
| Earlington Limited | Other services | 2025 |
| Earlyn's Kitchen Ltd | Hospitality | 2024 |
| Earthstone Resources (Europe) Ltd | Mining & quarrying | 2012 |
| East Beach Construction Limited | Construction | 2020 |
| East European Products at D&J Ltd | Hospitality | 2025 |
| East Kent Schoolboy Scramble Club Ltd | Other services | 2004 |
| Eastwoods Roofing Ltd | Construction | 2020 |
| Easy 2 Learn Driving School Ltd | Education | 2026 |
| Easy Option Limited | Health & social care | 2008 |
| Easy Steps Services Ltd | Administrative & support | 2021 |
| Easyaire Ltd | Energy | 2016 |
| Eatonclere Developments Limited | Real estate | 1972 |
| EAW Group Ltd | Other services | 2021 |
| Eazypay Ltd | Administrative & support | 2019 |
| Ebbi Care Ltd | Other services | 2018 |
| Ebbsfleet Accountants Limited | Professional & scientific | 2008 |
| Ebbsfleet Electrical Ltd | Construction | 2007 |
| Ebbsfleet Heating Ltd | Construction | 2017 |
| Ebbsfleet Internet Ltd | Extraterritorial | 2012 |
| Ebbsfleet United Football Club Limited | Arts & recreation | 1946 |
| Ebbsfleet Waste Management Ltd | Other services | 2020 |
| Ebenezer Enterprise Services (UK) Ltd | Other services | 2014 |
| Ebiripo & Ikokore Foods Festival Ltd | Hospitality | 2025 |
| EBM European Brand Management Ltd | Professional & scientific | 2016 |
| Ebog Limited | Health & social care | 2015 |
| Ebsolaz Ltd | Wholesale & retail | 2022 |
| Ec.Optimum Consulting Ltd | Health & social care | 2024 |
| EC3 Marine Limited | Financial services | 2012 |
| ECG Motors Ltd | Wholesale & retail | 2023 |
| Echad Cleaning Services Ltd | Administrative & support | 2025 |
| Echelon Maternity Limited | Real estate | 2013 |
| Echo Court (Gravesend) Limited | Real estate | 1992 |
| Echoz Community Interest Company | Construction | 2024 |
| Eclipse Air Conditioning Services Ltd | Construction | 2013 |
| Eclipse Integrated Services Limited | Construction | 2010 |
| ECN Construction Service Limited | Construction | 2017 |
| ECN Properties Limited | Real estate | 2025 |
| ECN Software Services Ltd | Information & communication | 2017 |
| Ecnellecxe Limited | Construction | 2024 |
| Eco Civils & Groundworks Ltd | Construction | 2024 |
| Eco Crew Ltd | Construction | 2020 |
| Eco Power Charge Ltd | Manufacturing | 2025 |
| ECO2 Green Ltd | Professional & scientific | 2023 |
| Ecoaire Ltd | Manufacturing | 2012 |
| Economics Online Ltd | Education | 2009 |
| Ecoviva Commercial Limited | Construction | 2008 |
| Ed's Traditional Barbers Ltd | Other services | 2021 |
| Eddan Studios Limited | Professional & scientific | 2024 |
| Eddie Martin Limited | Energy | 2014 |
| Eden Barber Rochester Limited | Other services | 2024 |
| Eden Business Consultancy Ltd | Professional & scientific | 2024 |
| Eden Estates and Properties Ltd | Real estate | 2024 |
| Eden Futures Ltd | Financial services | 2024 |
| Eden's Miracles Limited | Wholesale & retail | 2023 |
| Eden’S Cookies Ltd | Wholesale & retail | 2025 |
| Edgar Logistics Limited | Transport & logistics | 2025 |
| Edgley's Limited | Other services | 2025 |
| Edgy Websites Ltd | Information & communication | 2022 |
| Edible Foods Ltd | Wholesale & retail | 2025 |
| Edify Consultancy Limited | Education | 2006 |
| EDO Tech Ltd | Information & communication | 2024 |
| EDR Digital Ltd | Manufacturing | 2019 |