| Drummond Laurie (Holdings) Limited | Financial services | 2023 |
| Drummond Laurie Limited | Professional & scientific | 2013 |
| Drumossie Hotel Limited | Hospitality | 1996 |
| DS Process Ltd | Professional & scientific | 2018 |
| DSR Consulting Limited | Professional & scientific | 2022 |
| Duncan Data Science Ltd | Information & communication | 2024 |
| Dundas Foods Co Ltd | Hospitality | 2023 |
| Dwad Pro Ltd | Administrative & support | 2023 |
| DX2 Consulting Ltd | Information & communication | 2013 |
| Dynamic Golf Tours Limited | Administrative & support | 2017 |
| Earls Gate Developments Limited | Construction | 2023 |
| Earls Gate Gridco Limited | Other services | 2018 |
| Earls Gate Industrial Services Limited | Administrative & support | 2023 |
| Earls Gate Utilities Limited | Energy | 2023 |
| Earls Gate Waste Treatment Limited | Water & waste | 2023 |
| Earth Test Laboratories Ltd | Professional & scientific | 2022 |
| Easy Shop Africa Ltd | Wholesale & retail | 2024 |
| Easyip Ltd | Information & communication | 2007 |
| Easyways Walking Europe Ltd | Administrative & support | 2013 |
| EC Catering Ltd | Hospitality | 2021 |
| Ecosse Concrete Structures Limited | Construction | 2008 |
| ECS Developments Limited | Real estate | 2017 |
| Edesign Ltd | Professional & scientific | 2025 |
| Edinburgh Biosciences Ltd. | Manufacturing | 2003 |
| Edinburgh Tailoring Company Ltd | Wholesale & retail | 2019 |
| Effective Project Solutions Ltd. | Information & communication | 2008 |
| EJK Consulting Ltd | Information & communication | 2025 |
| Elite Endurance Nutrition Ltd | Education | 2026 |
| Elite Sporting Performance Ltd | Health & social care | 2011 |
| Elliott's Lettings Ltd | Real estate | 2022 |
| ELM Analysis Ltd | Administrative & support | 2018 |
| ELP Property Ltd | Real estate | 2020 |
| EM Quality Assurance Ltd. | Information & communication | 2025 |
| Emma Thomson Delivery Ltd | Transport & logistics | 2026 |
| Engenix Consulting Engineers Ltd | Professional & scientific | 2025 |
| Enterprise Development and Management Training Center Ltd | Professional & scientific | 2025 |
| Enviro-Core Solutions Limited | Water & waste | 2010 |
| Enviroclean Global Ltd | Wholesale & retail | 2018 |
| Enviroclean Holdings Ltd | Financial services | 2018 |
| EPC Direct Scotland Ltd | Professional & scientific | 2024 |
| Equi-Scotia CIC | Education | 2020 |
| ESB Property Developments Limited | Professional & scientific | 2019 |
| ESL Mcneish Consultancy Ltd | Professional & scientific | 2011 |
| ESP Physio Corporate Limited | Arts & recreation | 2019 |
| Eternal Elevators Limited | Manufacturing | 2013 |
| Ethos Health (Scotland) Ltd | Health & social care | 2014 |
| Eurofibre Networks Ltd | Information & communication | 2024 |
| Evie Wilkins Limited | Construction | 2023 |
| Exmos Employee Ownership Trustees Limited | Administrative & support | 2019 |
| Explearn Limited | Education | 2020 |
| Exploration Media Ltd | Information & communication | 2012 |
| Exterior Shield Property Care Ltd | Construction | 2025 |
| F I Coatings Limited | Manufacturing | 2010 |
| Fair Deal Tyres UK Ltd. | Extraterritorial | 2007 |
| Fairdeal Tyres Ltd. | Wholesale & retail | 2002 |
| Fairy Dogmother-Mobile Ltd | Other services | 2018 |
| Falconer Real Estate Ltd | Real estate | 2013 |
| Falkirk Building Company Limited | Construction | 2017 |
| Falkirk Independent Financial Consultants Limited | Administrative & support | 2004 |
| Falkirk Ltd | Real estate | 2022 |
| Falkirk Optical Ltd. | Health & social care | 2014 |
| Falkirk Properties Ltd | Real estate | 2021 |
| Fantastic Plastic Toys and Collectibles Ltd | Wholesale & retail | 2024 |
| Fanvill Ltd | Wholesale & retail | 2025 |
| Farid Travels Ltd | Transport & logistics | 2018 |
| Fario Ecom Ltd | Wholesale & retail | 2022 |
| Fario FLY Limited | Wholesale & retail | 2016 |
| Favorly Ltd | Professional & scientific | 2016 |
| Fegan Contracts Limited | Construction | 2012 |
| Fegan Plumbing & Heating Ltd | Construction | 2022 |
| Fencepoint Holdings Ltd | Financial services | 2023 |
| Fencepoint Ltd | Wholesale & retail | 2004 |
| Fentiman Investment Ltd | Administrative & support | 2018 |
| Feox Inspection Services Ltd | Professional & scientific | 2019 |
| Ferjani Solutions Ltd | Professional & scientific | 2022 |
| FFP Property Ltd | Real estate | 2020 |
| FFP1 Ltd | Wholesale & retail | 2020 |
| FFP6 Ltd | Wholesale & retail | 2020 |
| FGA Properties (Scotland) Ltd | Real estate | 2026 |
| FHA Consulting (UK) Limited | Professional & scientific | 2007 |
| FHF Ltd | Administrative & support | 2023 |
| FIC Training Ltd | Education | 2018 |
| Fife Rigging Consultants Ltd | Construction | 2025 |
| Filsell Wealth Ltd | Professional & scientific | 2021 |
| First 4 Frames Limited | Extraterritorial | 2014 |
| Fitcolab Ltd | Arts & recreation | 2026 |
| Fitzsimons Accountancy Services Limited | Professional & scientific | 2013 |
| FK10 Ltd | Hospitality | 2018 |
| FK3 Barber Ltd | Other services | 2025 |
| Flanigan Signs Limited | Manufacturing | 2015 |
| Fluid Performance Solutions Ltd | Professional & scientific | 2014 |
| Fone Unlocker Ltd | Wholesale & retail | 2023 |
| Forth & Clyde Marine Services Limited | Arts & recreation | 2005 |
| Forth Business Process Services Ltd | Administrative & support | 2023 |
| Forth Skip Repair and Refurbishers Ltd | Manufacturing | 2017 |
| Forward Communications Limited | Professional & scientific | 2016 |
| Fostering Relations Limited | Health & social care | 2007 |
| Foundry 23 Ltd | Information & communication | 2024 |
| Foundry Steels Ltd. | Water & waste | 2005 |
| Fowler Robben Limited | Professional & scientific | 2021 |