| A.C. Fullerton Limited | Wholesale & retail | 2003 |
| AGB Watson LLP | | 2017 |
| Aigle OPS Ltd | Information & communication | 2024 |
| Alba Kitchens Limited | Construction | 2002 |
| Andrew Findlay Consulting Ltd | Other services | 2019 |
| Barrel & Brew Ltd | Hospitality | 2023 |
| Bassendean Accountancy Services Limited | Administrative & support | 2001 |
| Bean 'N' Gone Borders Ltd | Hospitality | 2021 |
| Bell Storage Limited | Transport & logistics | 2005 |
| Borders Doula Ltd | Health & social care | 2024 |
| Cads 2021 Ltd | Construction | 2021 |
| Cleanliness Club Ltd | Other services | 2025 |
| Clover Cleaners Scotland Ltd | Administrative & support | 2024 |
| Cones and Candy Limited | Hospitality | 2013 |
| Craig RAE Ferguson Limited | Arts & recreation | 2020 |
| Croc Mhor Farms Limited | Agriculture & fishing | 2011 |
| DC Sportz Ltd | Wholesale & retail | 2018 |
| DFW 2013 Limited | Real estate | 2013 |
| DFW Properties Limited | Real estate | 2013 |
| DFW Storage Ltd | Real estate | 2025 |
| Drawerboxes Ltd | Wholesale & retail | 2014 |
| Fisherrow Outdoor Fitness Ltd | Education | 2026 |
| Healing Hands Home Care Company Ltd | Health & social care | 2023 |
| Health DAO Ltd | Education | 2019 |
| J & T F Macfarlane Limited | Agriculture & fishing | 1992 |
| JE Suis Prest Ltd | Hospitality | 2024 |
| Kelwest Ltd | Construction | 2020 |
| Kitchen Doors Plus Ltd | Wholesale & retail | 2015 |
| Life DAO Ltd | Education | 2019 |
| Mellerstain Garden | | 2000 |
| Opid Limited | Wholesale & retail | 2024 |
| Opulent Fleur Ltd | Wholesale & retail | 2025 |
| Quality Element Ltd | Professional & scientific | 2021 |
| R.R. Tweedie & Company Limited | Agriculture & fishing | 1959 |
| Reece Wilson Ltd | Arts & recreation | 2020 |
| Rhet Scottish Borders Countryside Initiative | Education | 2003 |
| Round Brand Communication Ltd | Professional & scientific | 2020 |
| Sawb Developments Limited | Construction | 2021 |
| Sleeper Collective Ltd | Information & communication | 2021 |
| THE Gordon Arms Ltd | Hospitality | 2018 |
| Three Hares Woodland CIC | Education | 2015 |
| Three Hills Coffee Company Limited | Wholesale & retail | 2016 |
| Tinknit Limited | Construction | 2012 |
| Vincent Coach Ltd | Real estate | 2021 |
| Watwish Properties Limited | Real estate | 2013 |
| Where to Stay Bookings Ltd | Real estate | 2024 |