| DTR Letting Limited | Real estate | 2020 |
| Duffield Developments Limited | Construction | 2021 |
| Dukes Group Holdings Limited | Financial services | 2025 |
| Dukes Holdco Limited | Financial services | 2023 |
| Dukes Property Management Limited | Real estate | 2025 |
| Duntulm Limited | Hospitality | 1978 |
| Durkan & Sons Holdings Ltd | Financial services | 2020 |
| Durkan & Sons Ltd | Construction | 2010 |
| Durkan & Sons Plant Hire Ltd | Administrative & support | 2014 |
| Durkan Homes Ltd | Construction | 2017 |
| Durkan Investments Limited | Financial services | 2024 |
| Durkin Electrical Ltd | Construction | 2023 |
| Dymondstrength Limited | Professional & scientific | 2010 |
| Dynamic Heating Solutions Ltd | Construction | 2026 |
| Dynamic Poptops Ltd | Manufacturing | 2025 |
| DZH03 Ltd | Hospitality | 2026 |
| E G Bates Ltd | Professional & scientific | 2014 |
| E-Vans & More Ltd | Transport & logistics | 2015 |
| E.S.B Enterprise Limited | Construction | 2025 |
| E.T.M. Training Limited | Education | 2014 |
| Eagle Leeds Ltd | Hospitality | 2023 |
| East Coast Sales Limited | Wholesale & retail | 2000 |
| East Riding Limited | Wholesale & retail | 2016 |
| East Yorkshire Caravan Salvage Limited | Wholesale & retail | 2009 |
| East Yorkshire Care Consultancy Limited | Professional & scientific | 2010 |
| East Yorkshire Community Transport Limited | Transport & logistics | 2008 |
| East Yorkshire Formwork & Reinforcement Ltd | Construction | 2026 |
| East Yorkshire MOT & Vehicle Repairs Ltd | Wholesale & retail | 2012 |
| Eastwood Civils & Groundworks Ltd | Construction | 2026 |
| Eastwood Contracting Ltd | Wholesale & retail | 2019 |
| Easy Glaze Goole Ltd | Manufacturing | 2025 |
| Easy Street Homes Ltd | Real estate | 2020 |
| EBI Medical Services Ltd | Health & social care | 2026 |
| EBS Renewables Ltd | Energy | 2013 |
| Echo.Hair Clinic Ltd | Other services | 2025 |
| Eco Building Supplies Ltd | Wholesale & retail | 2022 |
| Eco Future Heat Limited | Construction | 2022 |
| ECP Property Solutions Ltd | Real estate | 2024 |
| Ecsl Holdings Limited | Real estate | 2021 |
| ECU Rescue Limited | Other services | 2026 |
| EGD Freight Limited | Transport & logistics | 2018 |
| Eggs R US Ltd | Agriculture & fishing | 2002 |
| EGR Property Development Limited | Construction | 2020 |
| Elaine Johnson Fabrics & Interiors Ltd | Wholesale & retail | 2013 |
| Eldermere Investments Limited | Real estate | 1970 |
| Electech Engineering Limited | Manufacturing | 2002 |
| Electro Turbine Solutions Ltd | Manufacturing | 2024 |
| Elegant Life Homes Ltd | Administrative & support | 2026 |
| Elegant Life Parks Ltd | Other services | 2021 |
| Elite Fitness Goole Limited | Arts & recreation | 2021 |
| Elite Fitness Yorkshire Ltd | Arts & recreation | 2017 |
| Elite GAS Solutions Ltd | Construction | 2008 |
| ELK Pipework & Engineering Ltd | Professional & scientific | 2024 |
| Elliott's Residential Property Limited | Households | 2026 |
| Elowenrise Ltd | Wholesale & retail | 2025 |
| Emerald Veterinary Health Ltd | Professional & scientific | 2025 |
| Empire Print Machinery Limited | Wholesale & retail | 2012 |
| Emsa Property Limited | Real estate | 2009 |
| Emzam Ltd | Transport & logistics | 2019 |
| Energe Ltd | Professional & scientific | 2021 |
| Engage Occupational Therapy Limited | Extraterritorial | 2015 |
| Enterprise Coach Travel Ltd | Transport & logistics | 2025 |
| Enviro Advice Limited | Professional & scientific | 2021 |
| Environmental Compliance Consultants Ltd | Professional & scientific | 2020 |
| Environments for People Limited | Administrative & support | 2009 |
| Envirotek Ltd | Construction | 2023 |
| EOF Holdings Limited | Professional & scientific | 2012 |
| EOF Properties (UK) Ltd | Real estate | 2021 |
| EP Training and Assessments Ltd | Education | 2024 |
| EPC Compliance & Assessments Ltd | Professional & scientific | 2026 |
| ERT Solutions Limited | Professional & scientific | 2025 |
| Escape Rooms for YOU Ltd | Administrative & support | 2020 |
| Escort AID Limited | Transport & logistics | 2016 |
| ESK Building Control Ltd | Professional & scientific | 2016 |
| Essex Cement Limited | Transport & logistics | 2020 |
| Euro Food Goole Limited | Wholesale & retail | 2023 |
| European & British Equestrian Distribution Ltd | Wholesale & retail | 2018 |
| European Mini Market (Goole) Limited | Wholesale & retail | 2020 |
| Eurotom Trans Ltd | Transport & logistics | 2018 |
| EV Cable Tidy Ltd | Wholesale & retail | 2025 |
| EV Tyre Services Limited | Wholesale & retail | 2024 |
| Evaspire Ltd | Wholesale & retail | 2021 |
| Evatrans Ltd | Transport & logistics | 2024 |
| Everlong Property Ltd | Real estate | 2025 |
| Everspire Services Ltd | Administrative & support | 2024 |
| Everwear Ltd | Wholesale & retail | 2025 |
| Evolution Designers LLP | | 2023 |
| Exchange Trading Company Limited | Wholesale & retail | 2005 |
| Eximia Social Care Limited | Public administration | 2025 |
| Expressions Cheerleading Academy CIC | Arts & recreation | 2023 |
| External Impression Ltd | Wholesale & retail | 2025 |
| F E G Promotions Limited | Professional & scientific | 2024 |
| F Martinson and SON Limited | Administrative & support | 2023 |
| F.W.Shaw & Son,Limited | Agriculture & fishing | 1947 |
| Falcons Learning Ltd | Education | 2021 |
| Family Autos Ltd | Wholesale & retail | 2011 |
| Fantique Limited | Wholesale & retail | 2025 |
| FBF Consulting Ltd | Information & communication | 2025 |
| Feature Wall Art Limited | Wholesale & retail | 2014 |
| Feel Good Fitness Howden Ltd | Arts & recreation | 2023 |