| Anna's Happy Trotters Limited | Manufacturing | 2008 |
| Ansym Estates Limited | Real estate | 2014 |
| APB Blade Repair Services Ltd | Information & communication | 2023 |
| Apdr. Ltd | Education | 2025 |
| Apisterra Limited | Agriculture & fishing | 2026 |
| Aquastars Hull Ltd | Education | 2022 |
| Aquastars Swim Academy Ltd | Education | 2022 |
| Aquatania Limited | Information & communication | 1995 |
| ARC Labels Limited | Manufacturing | 1983 |
| Archer UK Business Consultancy Ltd | Professional & scientific | 2014 |
| Arek & Artur Company Ltd | Transport & logistics | 2019 |
| Arello Construction Ltd | Professional & scientific | 2017 |
| Argent Catering Services Ltd | Hospitality | 2020 |
| Argyll Drummond Financial Services Ltd | Financial services | 2021 |
| Aries ENG Ltd | Transport & logistics | 2024 |
| Arkanoid Limited | Manufacturing | 1992 |
| Arnoldlifters Ltd | Manufacturing | 2016 |
| Arnoldlinks Ltd | Information & communication | 2015 |
| Art of Shape Ltd | Other services | 2026 |
| Arthur Mining Support Services Ltd | Mining & quarrying | 2021 |
| Arturs Cildermanis Services Limited | Construction | 2026 |
| Ashes Associates Limited Liability Partnership | | 2006 |
| Ashley Aesthetics Ltd | Health & social care | 2021 |
| Ashmuir | Financial services | 2021 |
| Ashtree Rescue and Rehabilitation Centre Ltd | Agriculture & fishing | 2024 |
| ASL Telecoms North Ltd | Construction | 2018 |
| Aspire Engineering Solutions Ltd | Manufacturing | 2016 |
| Asprey Brothers Deluxe Ltd | Real estate | 2023 |
| Atlas Hydrocarbons Limited | Professional & scientific | 2007 |
| AU Bargains UK Ltd | Wholesale & retail | 2023 |
| Augscape Engagements (AR) Limited | Information & communication | 2015 |
| Augscape Research & Development Limited | Information & communication | 2020 |
| Augscape Studios Limited | Information & communication | 2014 |
| Aure Ltd | Wholesale & retail | 2024 |
| Austin Aesthetics Ltd | Health & social care | 2022 |
| Austwick Haulage Ltd | Transport & logistics | 2010 |
| Auto-Tu Ltd | Wholesale & retail | 2018 |
| Autoend Ltd | Wholesale & retail | 2023 |
| Avalanche Telecoms Limited | Information & communication | 2018 |
| Avanta Guard Ltd | Administrative & support | 2025 |
| Awards Production Ltd | Arts & recreation | 2016 |
| Ayan MK Ltd | Wholesale & retail | 2025 |
| B a Johnson Ltd | Construction | 2020 |
| B Animal Feeds Limited | Manufacturing | 2017 |
| B Aqua and Animal Feeds Limited | Arts & recreation | 2015 |
| B M Lynch Ltd | Professional & scientific | 2026 |
| B&A Brownbridge Ltd | Agriculture & fishing | 2021 |
| B&K Barber Ltd | Other services | 2022 |
| B. D. Snaith Limited | Households | 2009 |
| B. Webster and Sons (Eastrington) Limited | Agriculture & fishing | 1978 |
| B.H.B. (Goole) Limited | Manufacturing | 1995 |
| Babe-Equip.Co.Uk Limited | Wholesale & retail | 2009 |
| Badger & Bean Ltd | Hospitality | 2023 |
| Badger @ NO 7 Ltd | Wholesale & retail | 2025 |
| Bakeology Ltd | Professional & scientific | 2015 |
| Baker & Baker Properties Ltd | Construction | 2024 |
| Bakes ME Happy Limited | Manufacturing | 2021 |
| Balne Logs Ltd | Wholesale & retail | 2022 |
| BALU41 Limited | Transport & logistics | 2023 |
| Bankside (Distribution) Limited | Transport & logistics | 2013 |
| Baptista Machinery Procurement Limited | Wholesale & retail | 2025 |
| Bark N Bubbles Limited | Other services | 2024 |
| Barker Coleman Developments Ltd | Construction | 2019 |
| Barlows Joinery Ltd | Construction | 2020 |
| Barnsley One Ltd | Other services | 2017 |
| Barras Industries Ltd | Information & communication | 2024 |
| Barrett Site Services Ltd | Construction | 2021 |
| Barry Mowforth Limited | Agriculture & fishing | 1984 |
| Baseline Office Furniture Limited | Manufacturing | 2016 |
| Baseline Office Holdings Limited | Financial services | 2023 |
| Baseline Property Limited | Real estate | 2023 |
| Basic Consult Ltd | Information & communication | 2024 |
| Bathrooms 4 U North Ltd | Construction | 2016 |
| BB Fitness and Rehabilitation Ltd | Other services | 2012 |
| Bcjms Limited | Construction | 2015 |
| BE Rude NOT to Ltd | Manufacturing | 2018 |
| Beau Boutique Limited | Wholesale & retail | 2021 |
| BEK Fish Ltd | Hospitality | 2023 |
| Bellasize Limited | Energy | 2014 |
| Beltflex Ltd | Manufacturing | 2015 |
| Bennett's Baby Boutique Ltd | Wholesale & retail | 2023 |
| Bensley Ltd | Professional & scientific | 2026 |
| Benson Inns Limited | Hospitality | 2019 |
| BESA777 Ltd | Hospitality | 2025 |
| Best Barber (Goole) Limited | Other services | 2023 |
| Best One Mini Market(Goole) Limited | Wholesale & retail | 2023 |
| Bestpak Limited | Administrative & support | 2019 |
| Between Rivers Limited | Hospitality | 2025 |
| Bich Vintage Limited | Wholesale & retail | 2014 |
| Bidfuel Limited | Information & communication | 2026 |
| Big Ambitions CIC | Administrative & support | 2010 |
| Big Bale Holdings Limited | Financial services | 2018 |
| Big Bale Services Limited | Manufacturing | 2005 |
| Big Picture (Software) Limited | Information & communication | 1999 |
| Biig Bites Limited | Hospitality | 2021 |
| BIO Futures PI Limited | Professional & scientific | 2003 |
| Bistro Ventures Limited | Financial services | 2026 |
| Bizon Transport Ltd | Transport & logistics | 2016 |
| Black 90 Limited | Wholesale & retail | 2018 |
| Black and White Design Limited | Hospitality | 1992 |