| Fredk. Allen (Wholesale) Limited | Wholesale & retail | 1949 |
| Freebrights Limited | Wholesale & retail | 1998 |
| Freeman Electrical & Security Limited | Construction | 2002 |
| G P Decorations Limited | Construction | 2002 |
| G P Hose Services Limited | Manufacturing | 1995 |
| G.B. Land Limited | Construction | 1988 |
| G.I. Markey & SON Ltd | Manufacturing | 2002 |
| Gardner Schunemann Limited | Professional & scientific | 2002 |
| Gecko Technology Ltd | Information & communication | 2007 |
| Geoffs Tyres Limited | Wholesale & retail | 2003 |
| George Bence & Sons (Tewkesbury) Limited | Wholesale & retail | 1984 |
| GINGER49 Limited | Real estate | 2002 |
| Gino Fatica Limited | Wholesale & retail | 2003 |
| GL1 Management Company Ltd | Households | 2008 |
| Global Footsteps | Education | 1985 |
| Global Furniture Alliance Limited | Administrative & support | 2004 |
| Global Vegans Ltd | Information & communication | 2015 |
| Gloucester Paint Supply Company Limited | | 1985 |
| Gloucester Scaffolding Limited | Construction | 1992 |
| Gloucestershire Everyman Theatre Catering Company Limited | Hospitality | 1992 |
| Gloucestershire Everyman Theatre Company Limited | Arts & recreation | 1960 |
| Gnosis Consultancy Limited | Information & communication | 1998 |
| Go-Race Engineering Ltd | Professional & scientific | 2017 |
| Goff's Brewery Limited | Manufacturing | 1993 |
| Golden Green (UK) Ltd | Hospitality | 2005 |
| Goode Projects Limited | Professional & scientific | 2002 |
| Graeme Cooper Chiropractic Limited | Health & social care | 2007 |
| Graham Ross Consulting Services Limited | Information & communication | 2005 |
| Gray Surveying Limited | Professional & scientific | 2006 |
| Green Farm Seeds Limited | Agriculture & fishing | 2004 |
| Green Flow Heating and Plumbing Limited | Construction | 2004 |
| Ground Control Technologies UK Ltd | Information & communication | 2004 |
| GRP Electrical (UK) Ltd | Construction | 2005 |
| GSM Limited | Manufacturing | 1995 |
| GSS Direct Ltd | Wholesale & retail | 2007 |
| Gyles Allen Ltd | Other services | 2002 |
| H W Fine Art Limited | Wholesale & retail | 2017 |
| H.F. Hart Limited | Agriculture & fishing | 1973 |
| Hage Limited | Information & communication | 2007 |
| Half Moon Plantation Limited | Transport & logistics | 2025 |
| Halford Associates Limited | Professional & scientific | 2005 |
| Hanstone Court Management Company Limited | Real estate | 1988 |
| Happy Days Gym Club Ltd | Arts & recreation | 2007 |
| Harani Hair Limited | Other services | 2003 |
| Harbour Road Electrical Limited | Construction | 2006 |
| Haremi Employees Trustees Limited | Extraterritorial | 2020 |
| Haremi Limited | Education | 2006 |
| Harrison & Hardie Limited | Real estate | 1999 |
| Harrison Close Management Company Limited | Real estate | 1995 |
| Hartley and Tissier (UK) Limited | Manufacturing | 2017 |
| Hartpury College of Further Education | Other services | 2018 |
| Hartwell and Company (Timber) Ltd. | Wholesale & retail | 2002 |
| Hawkes Pharmacy 2007 Limited | Health & social care | 2007 |
| Haywards (Tewkesbury) Limited | Wholesale & retail | 1934 |
| HCI Electrical Limited | Administrative & support | 2004 |
| Helyx Consultants Ltd | Information & communication | 2003 |
| Henge Software Construction Ltd | Extraterritorial | 2006 |
| Hengst (North Cotswold)Limited | Real estate | 1967 |
| HG Mind Works Ltd | Health & social care | 2025 |
| HHC Ltd | Construction | 2006 |
| High Tech Fabrications Limited | Manufacturing | 2001 |
| Higher Education Quality Consultancy Limited | Education | 2007 |
| Hillview Evangelical Church | Other services | 2006 |
| Histed Haulage Limited | Transport & logistics | 2002 |
| Hock Enterprises Limited | Real estate | 2000 |
| Hollingsworth & Vose Company Limited | Manufacturing | 1900 |
| Hooper Bolton Limited | Wholesale & retail | 2002 |
| Hope Property Developments (Bristol) Limited | Construction | 2021 |
| Howdah Limited | Transport & logistics | 2002 |
| HP Decor Limited | Other services | 2007 |
| Hudgemoor Consulting Ltd | Manufacturing | 2019 |
| Hydegate Pet Resort Limited | Administrative & support | 2006 |
| IDM Limited | Information & communication | 1997 |
| IFC Wire Components Limited | Wholesale & retail | 2006 |
| Imconsultancy Limited | Information & communication | 1998 |
| In-Parallel Computer Staff Ltd. | Administrative & support | 1995 |
| Inn Company Management Limited | Administrative & support | 2000 |
| Innotive Vision Management Limited | Administrative & support | 2025 |
| Innsworth Technology Park Limited | Construction | 1982 |
| Inshape Stroud Limited | Construction | 2006 |
| Intersound Music Limited | Wholesale & retail | 2003 |
| Into the Green Ltd | Education | 2018 |
| Iron & Earth Holdings Ltd | Wholesale & retail | 2022 |
| Isis Computer Systems (Abingdon) Limited | | 1982 |
| ISL (R&D) Ltd | Manufacturing | 2008 |
| Issuemade Limited | Construction | 1991 |
| Itpot Limited | Wholesale & retail | 2018 |
| Itsfitz Limited | Professional & scientific | 2006 |
| J N Renovations Ltd | Construction | 2023 |
| J&L Cleaning Services Ltd | Administrative & support | 2024 |
| J.Broomhall Limited | Wholesale & retail | 1969 |
| James Ford Design Ltd. | Professional & scientific | 1988 |
| James Moore Media Ltd | Arts & recreation | 2007 |
| Janet Marshall Limited | Education | 2002 |
| Jaxtech Limited | Information & communication | 1989 |
| JAY City Finance Limited | Financial services | 1997 |
| JAY City Leasing Ltd | Financial services | 2001 |
| JIM Kimber Limited | Administrative & support | 2003 |
| Jireh Solutions Limited | Information & communication | 2006 |
| Jmjc Ltd | Wholesale & retail | 2005 |