| Retroclassic Engineering Ltd | Manufacturing | 2020 |
| Retroclassic Restoration Limited | Transport & logistics | 2020 |
| Retweets Ltd | Hospitality | 2020 |
| Revive! Gloucester Ltd | Wholesale & retail | 2021 |
| Revived Leads Limited | Information & communication | 2024 |
| Revolution Air Services Ltd | Professional & scientific | 2011 |
| REW Solutions Ltd | Wholesale & retail | 2017 |
| Rewardrite Limited | Professional & scientific | 2024 |
| Rewards Admin Ltd | Information & communication | 2025 |
| Rewear Wewear Ltd | Wholesale & retail | 2025 |
| Reweb Financial Services Limited | Extraterritorial | 2025 |
| REX Bishop & SON Ltd | Construction | 2007 |
| RF Harries Ltd | Construction | 2021 |
| RGD Plumbing & Heating Ltd | Construction | 2023 |
| RGP Building Ltd | Construction | 2026 |
| RGS Housing Ltd | Real estate | 2023 |
| RH Building Systems Ltd | Construction | 2021 |
| RH Carpentry & Property Maintenance Ltd | Administrative & support | 2026 |
| RH Diamond Properties Ltd | Real estate | 2025 |
| RH Eco Hotel Limited | Hospitality | 2014 |
| RH Plastics Ltd | Wholesale & retail | 2013 |
| Rhadamanthus Design LLP | | 2018 |
| Rhapsody Nails Treatment Ltd | Other services | 2025 |
| RHM Telecommunications Limited | Information & communication | 1999 |
| Rhodel Company Limited | Wholesale & retail | 2010 |
| Rhodes Carpentry Limited | Construction | 2007 |
| Rhys Evans Property Holdings Ltd | Real estate | 2024 |
| Ribston Hall High School Academy Trust | Education | 2011 |
| Ribston Hall Management Company Limited | Households | 2003 |
| Rich Leigh and Company Ltd | Professional & scientific | 2014 |
| Richard Guest Property Limited | Real estate | 2025 |
| Richard Thwaites Aviation Limited | Administrative & support | 2002 |
| Richmond Forwarding Limited | Extraterritorial | 2008 |
| Richmondway Contracts Limited | Hospitality | 1998 |
| Richmondway Management Limited | Administrative & support | 2015 |
| Rickard Interiors Limited | Construction | 2002 |
| Rickards Construction Ltd | Real estate | 1999 |
| Rickards Developments Ltd | Construction | 2019 |
| Rickards Holdings Ltd | Real estate | 2021 |
| Rideincity Limited | Information & communication | 2020 |
| Ridgemoor Developments Limited | Real estate | 2006 |
| Ridgemoor Holdings Limited | Real estate | 2006 |
| Rijki Digital Ltd | Information & communication | 2025 |
| Rinaldi Bakes Limited | Manufacturing | 2024 |
| Risesi Limited | Information & communication | 2021 |
| Risha Solutions Limited | Hospitality | 2025 |
| Risk Advisory Limited | Professional & scientific | 2012 |
| Rival Creative Limited | Professional & scientific | 2019 |
| Riverside Green Management Company NO. 1 Limited | Households | 1991 |
| Riverside Sports and Leisure Ltd | Arts & recreation | 2003 |
| Rivi Limited | Real estate | 2004 |
| Riya Sparkle Limited | Hospitality | 2024 |
| RIZ Global Ventures Limited | Wholesale & retail | 2023 |
| RJC Maintenance Ltd | Construction | 2024 |
| RJD Hardwicke Holdings Ltd | Financial services | 2026 |
| RJL Gloucester Limited | Transport & logistics | 2008 |
| RJM Electrical Contractors Limited | Construction | 2019 |
| RJP Nuclear Associates Limited | Professional & scientific | 2022 |
| RJP Property Consultants Ltd | Real estate | 2014 |
| RJW Group Ltd | Construction | 2017 |
| RK Motors Cars Ltd | Wholesale & retail | 2025 |
| RK PVT Ltd | Wholesale & retail | 2019 |
| RKC Energy Systems Limited | Manufacturing | 2000 |
| RKM Retail Stores Ltd | Wholesale & retail | 2024 |
| RL Architecture Ltd | Professional & scientific | 2014 |
| RLC Transport Solutions Ltd | Transport & logistics | 2025 |
| Rlconstruction Team Limited | Construction | 2024 |
| RLW Homes Ltd | Real estate | 2025 |
| RM Project Consulting Ltd | Construction | 2018 |
| RMV Videos Limited | Wholesale & retail | 2020 |
| Road Safety Training Ltd | Education | 2024 |
| Road to M Ltd | Wholesale & retail | 2026 |
| Roadar Limited | Extraterritorial | 2016 |
| Roadrunner Pizza Ltd | Hospitality | 2013 |
| Roam & Wonder Foundation CIC | Education | 2025 |
| Robb & Robb Ltd | Health & social care | 2021 |
| Robbins Building Services Limited | Construction | 2011 |
| Roben Design Limited | Manufacturing | 2024 |
| Robert Colthart Car Sales Limited | Wholesale & retail | 2001 |
| Robert Deacon Builders Limited | Construction | 1985 |
| Robert Deacon Holdings Limited | Financial services | 1997 |
| Robert Hall Business Equipment Limited | Wholesale & retail | 1991 |
| Robert Hall Group Ltd | Wholesale & retail | 2017 |
| Robert Hall Interior Design Limited | Wholesale & retail | 2017 |
| Robert Hall Office Supplies Ltd | Wholesale & retail | 2017 |
| Robert Hall Technology Solutions Limited | Wholesale & retail | 2017 |
| Robert Powell Accountants Ltd | Professional & scientific | 2011 |
| Robert Street & Sons (Contractors) Limited | Real estate | 1977 |
| Roberts Civil Engineers Limited | Extraterritorial | 2004 |
| Roberts Farms (Glos) Limited | Administrative & support | 2004 |
| Roberts Limbrick Holdings Limited | Professional & scientific | 2008 |
| Roberts Limbrick Limited | Professional & scientific | 2008 |
| Roberts Plant Engineers Limited | Administrative & support | 2000 |
| Robin Lamb Independent Mercedes Specialists Limited | Wholesale & retail | 2006 |
| Robin's Bastion Ltd | Wholesale & retail | 2022 |
| Robinson Canicross and PT Limited | Education | 2018 |
| Robinson Contracting Company Ltd | Construction | 2022 |
| Robinson David Ltd | Real estate | 2021 |
| Robinswood Building Solutions Ltd | Construction | 2018 |
| Roblucos Ltd | Professional & scientific | 2020 |