| Orizaba Technology Ltd | Administrative & support | 2021 |
| Orkos Property Ltd | Real estate | 2020 |
| Orriss Consult Ltd | Professional & scientific | 2019 |
| Ortarna Enterprises Ltd | Wholesale & retail | 2021 |
| Osborne Lodge (Cheltenham) Limited | Real estate | 1995 |
| Oscar Deen Ventures Ltd | Other services | 2017 |
| Oscar John Limited | Financial services | 2026 |
| Osita Limited | Health & social care | 2015 |
| Osteomaya Ltd | Health & social care | 2024 |
| Otto's Antiques Limited | Wholesale & retail | 2021 |
| Ottsworkshop Limited | Manufacturing | 2022 |
| OUR Real Estate Limited | Real estate | 2026 |
| OUT & OUT Inns Limited | Hospitality | 1999 |
| OUT of the Woods Forestry Services Limited | Agriculture & fishing | 2014 |
| Outside Edge Property Limited | Real estate | 2020 |
| Ovelia Ltd | Professional & scientific | 2025 |
| Over Farm Microlights Limited | Other services | 2020 |
| Over Farm Retail Limited | Wholesale & retail | 2011 |
| Overbrook (Barnwood) Limited | Households | 1974 |
| Overhead Evaluations Ltd | Professional & scientific | 2025 |
| Overnight Transformations Ltd | Arts & recreation | 2025 |
| Overton (Gloucester) Limited | Administrative & support | 2004 |
| Overture Installations Limited | Professional & scientific | 2011 |
| Owen Scorer Media Ltd | Manufacturing | 2023 |
| Owilima Solutions Ltd | Health & social care | 2024 |
| Ownside Limited | Real estate | 1996 |
| OXA Limited | Real estate | 1996 |
| Oxalis Trading Limited | Professional & scientific | 2002 |
| Oxford Headache Limited | Health & social care | 2018 |
| Oxford Street Gloucester Management Company Limited | Households | 2005 |
| Oxstalls Service Station Limited | Wholesale & retail | 1986 |
| OXX Group Ltd | Administrative & support | 2022 |
| Oyenna Limited | Wholesale & retail | 2021 |
| P & J Glos Limited | Wholesale & retail | 2020 |
| P & L Manufacturing Limited | Manufacturing | 2005 |
| P & M Group Limited(The) | Manufacturing | 1986 |
| P & P Care Homes Limited | Health & social care | 2009 |
| P & R Synergy Holdings Limited | Professional & scientific | 2004 |
| P & T Wardle Limited | Administrative & support | 2025 |
| P and H Mansell Limited | Construction | 2021 |
| P Gunzo Limited | Transport & logistics | 2019 |
| P H Electrical Limited | Construction | 2003 |
| P H Recovery Ltd | Transport & logistics | 2023 |
| P J Lane Limited | Manufacturing | 2017 |
| P J Mcgurk Limited | Extraterritorial | 2018 |
| P P Electrics (Gloucester) Limited | Wholesale & retail | 1990 |
| P S Mitchell Roofing Ltd | Construction | 2017 |
| P&F Management Limited | Real estate | 2021 |
| P&H Enterprises Ltd | Wholesale & retail | 2022 |
| P&J Management Limited | Real estate | 2022 |
| P&M Used Car Sales Ltd | Wholesale & retail | 2023 |
| P&T Global Ltd | Wholesale & retail | 2025 |
| P.A. COX Limited | Construction | 2008 |
| P.A.C.E. Gloucester Ltd | Health & social care | 2017 |
| P.Bailey and Co Holdings Ltd | Financial services | 2022 |
| P.D. Roberts Contractors Limited | Construction | 2003 |
| P.J. Rugg Limited | Administrative & support | 1991 |
| P.P Engineering (Stroud) Limited | Professional & scientific | 2016 |
| P.T. Interiors UK Limited | Professional & scientific | 2005 |
| P.W Luxury TUB Hire Ltd | Administrative & support | 2026 |
| P1 Project Management Ltd | Professional & scientific | 2023 |
| P4P Compliance Management Limited | Information & communication | 2009 |
| P88 Pest Control Ltd | Administrative & support | 2024 |
| PA Performance Limited | Wholesale & retail | 2002 |
| Paac Housing CIC | Real estate | 2025 |
| Paarthiv Info Tech Limited | Information & communication | 2014 |
| Pablotrans 2 Limited | Transport & logistics | 2017 |
| PAC Property Limited | Real estate | 2016 |
| Pacc-Holdings UK Ltd | Real estate | 2022 |
| Pachira Trader Ltd | Wholesale & retail | 2022 |
| Packet Company Services Limited | Information & communication | 2024 |
| Padel Build UK Limited | Manufacturing | 2024 |
| Padova Ltd | Real estate | 2012 |
| Padpilot Ltd | Administrative & support | 2010 |
| Paige of Sussex Limited | Manufacturing | 2022 |
| Paintworks 2023 Limited | Wholesale & retail | 2023 |
| Paish Tooth Limited | Professional & scientific | 2003 |
| Paje Health and Care Limited | Health & social care | 2023 |
| PAK Vintage Ltd | Wholesale & retail | 2025 |
| Pallas Systems Ltd | Information & communication | 2022 |
| Palmer & Associates Limited | Financial services | 2000 |
| Palmflow Studio Limited | Other services | 2025 |
| Palsep Limited | Manufacturing | 1982 |
| Pamo Enterprise Ltd | Transport & logistics | 2023 |
| Panacea Business Systems Limited | Wholesale & retail | 2013 |
| Panax Investments Ltd | Real estate | 2022 |
| Panda Epos Limited | Information & communication | 2016 |
| Panda Recycling Ltd | Wholesale & retail | 2016 |
| Panem in Aquas Ltd | Administrative & support | 2025 |
| Panex Limited | Administrative & support | 1997 |
| Pangaea Consulting Limited | Information & communication | 2000 |
| Pangaea Developments Limited | Information & communication | 2011 |
| Pankun Limited | Wholesale & retail | 2025 |
| Panoo-Ant Limited | Transport & logistics | 2025 |
| Pantech IT Ltd | Information & communication | 2024 |
| Panting Properties Limited | Real estate | 2021 |
| Panting Property Development Limited | Real estate | 2021 |
| Paolini Property Ltd | Real estate | 2023 |
| Paperchem Consultants Limited | Professional & scientific | 2022 |
| Paperclips Included Ltd | Administrative & support | 2019 |