| Nena Services Limited | Households | 2016 |
| NEO - Tongue-Tie and Lactation Ltd | Health & social care | 2026 |
| Neocentric Limited | Information & communication | 2007 |
| Neolink Processing Systems Ltd | Information & communication | 2024 |
| Neomill Ltd | Wholesale & retail | 2024 |
| Neon Junkie Ltd | Arts & recreation | 2016 |
| Nepalese Chef Limited | Hospitality | 2013 |
| Neptune Building Services (Holdings) Ltd | Financial services | 2020 |
| Neptune Building Services Ltd | Construction | 1984 |
| Neptune Publishing Ltd | Information & communication | 2021 |
| Neptune Radar Limited | Professional & scientific | 1983 |
| Neptunes Takeaway Ltd | Wholesale & retail | 2010 |
| Nerva Homes Ltd | Construction | 2018 |
| NES Contract Ltd | Administrative & support | 2025 |
| Nestara Realty Ltd | Real estate | 2025 |
| NET9DESIGN Ltd | Information & communication | 2000 |
| Netbytes Ltd | Information & communication | 2025 |
| Netherstone Limited | Wholesale & retail | 2025 |
| Netherwood Lane Limited | Administrative & support | 2017 |
| Nethra Academy Ltd | Arts & recreation | 2023 |
| Netronix Tech Ltd | Construction | 2024 |
| Network Electricity Limited | Construction | 2025 |
| Neuronx Limited | Administrative & support | 2016 |
| Neuwave Therapies Ltd | Other services | 2024 |
| Neverland Childcare Limited | Health & social care | 2018 |
| Neves and Wire Associates Ltd | Professional & scientific | 2013 |
| New Beginnings (Gloucester) Ltd. | Health & social care | 2001 |
| New Biedronki Ltd | Wholesale & retail | 2022 |
| New Clear Vision Ltd | Real estate | 2014 |
| New College Developments Limited | Energy | 2016 |
| New Dawn Financials Ltd | Financial services | 2025 |
| New Dorset Limited | Education | 2019 |
| New Empire Enterprise Ltd | Construction | 2018 |
| New Fundraising Experience Limited | Other services | 2016 |
| New GEN (UK) Limited | Professional & scientific | 2009 |
| New Gentis Hand Car Wash Ltd | Wholesale & retail | 2024 |
| New Horizons (Gloucester) Limited | Health & social care | 2003 |
| New Inn Gloucester Ltd | Hospitality | 2022 |
| New JIN LE Limited | Hospitality | 2024 |
| New Leaf Supported Living Limited | Health & social care | 2017 |
| New Medicine Consulting Ltd | Other services | 2020 |
| New Photography Experience Ltd | Professional & scientific | 2022 |
| New Property Experience Ltd | Hospitality | 2022 |
| New Road Foods Limited | Administrative & support | 2026 |
| New Ruby 2018 Limited | Hospitality | 2024 |
| New Start Living Ltd | Other services | 2020 |
| New Start Living Residential Ltd | Health & social care | 2023 |
| New Start Supported Housing | Real estate | 2009 |
| New Trent Services Ltd | Real estate | 2025 |
| New World Shipping &Cargo Ltd | Wholesale & retail | 2023 |
| Newcup Limited | Manufacturing | 2022 |
| Newell Courier Services Limited | Transport & logistics | 2019 |
| Newfangled Engineering Ltd | Professional & scientific | 2019 |
| Newgrade Ltd | Construction | 2013 |
| Newhall Jones Ltd | Professional & scientific | 2016 |
| Newhall Veterinary Surgery Ltd | Professional & scientific | 2024 |
| Newland Homes Limited | Construction | 1991 |
| Newland on the Common Management Company Limited | Real estate | 2013 |
| Newman Construction & Sons Ltd | Construction | 2019 |
| Newman Dog Grooming Ltd | Other services | 2024 |
| Newport and Lane Property Limited | Construction | 2020 |
| Newport House Management Company Limited | Households | 1989 |
| Newray Property Ltd | Real estate | 2023 |
| Newtown Properties Limited | Real estate | 1989 |
| Newtrality Ltd | Professional & scientific | 2021 |
| Next GEN Nuclear Limited | Professional & scientific | 2021 |
| Next Generation Nuclear Limited | Professional & scientific | 2021 |
| Next Level Accountants Limited | Professional & scientific | 2020 |
| Next Level Software Solutions Limited | Wholesale & retail | 2024 |
| Next Motor Group Ltd | Wholesale & retail | 2025 |
| Next Wheels and Tyres Ltd | Wholesale & retail | 2020 |
| Nextverk Limited | Wholesale & retail | 2026 |
| Nexus Solve Ltd | Professional & scientific | 2025 |
| Nfotech Solution Limited | Information & communication | 2022 |
| NFP Trading Limited | Wholesale & retail | 1997 |
| NG Nuclear Limited | Professional & scientific | 2021 |
| NG Recreation Ltd | Arts & recreation | 2026 |
| NGR Interiors Limited | Construction | 2022 |
| Ngubane Holdings Limited | Financial services | 2025 |
| NH Groundworks & Landscaping Ltd | Construction | 2026 |
| Niche Media and SEO Ltd | Information & communication | 2022 |
| Niche RF Ltd | Manufacturing | 2024 |
| Nick King Car Sales Limited | Wholesale & retail | 2002 |
| Nicks & Company(Timber)Limited | Agriculture & fishing | 1960 |
| Nigel Bannerman Services Limited | Financial services | 2019 |
| Nigerian Association Gloucestershire CIC | Professional & scientific | 2025 |
| Night Wraith Studio Ltd | Information & communication | 2026 |
| Nightingale and Balster Developments Limited | Construction | 2025 |
| Nightingales Area H75 Haydon Wick Management Company Limited | Households | 2006 |
| Nightingales Care (Gloucester) Limited | Health & social care | 2009 |
| Nightingales Care (Holdings) Limited | Professional & scientific | 2014 |
| Nightshift Films Ltd | Information & communication | 2003 |
| Niki Ellis Property Ltd | Real estate | 2023 |
| Nile Prime Limited | Health & social care | 2023 |
| Nimbaru Ltd | Wholesale & retail | 2025 |
| Nimbulus Consulting Ltd | Information & communication | 2024 |
| Nimi Stores Ltd | Wholesale & retail | 2025 |
| Nimish Consultancy Limited | Information & communication | 2025 |
| Nimit Ltd | Wholesale & retail | 2023 |
| Nimo's Childcare Limited | Health & social care | 2026 |