| JJW Developments Limited | Construction | 2004 |
| JK Property (Gloucester) Ltd | Real estate | 2021 |
| JKA Financial Planning Limited | Financial services | 2011 |
| JKA Services Limited | Wholesale & retail | 2025 |
| JKV Refrigeration and Air Conditioning Limited | Construction | 2023 |
| Jlfam Ltd | Wholesale & retail | 2025 |
| JLG IT Solutions Limited | Information & communication | 2019 |
| JLP IT Consultancy Ltd | Information & communication | 2019 |
| Jmcg Digital Ltd | Information & communication | 2024 |
| Jmetals Limited | Wholesale & retail | 2014 |
| Jmhg Limited | Hospitality | 2023 |
| JMJ Building & Restoration Ltd | Construction | 2021 |
| JMK Mortgage Solutions Ltd | Financial services | 2026 |
| Jmlm Properties Limited | Real estate | 2025 |
| JMP Homes Ltd | Real estate | 2025 |
| JMS Innovations Ltd | Real estate | 2025 |
| JNO Construction Ltd | Construction | 2025 |
| JNS Arts Limited | Wholesale & retail | 2020 |
| JO Cratchley Compliance Ltd | Professional & scientific | 2024 |
| Joanna James Limited | Information & communication | 2017 |
| Jobbl Ltd | Administrative & support | 2016 |
| JOE Assets Limited | Construction | 2014 |
| JOE Edwards Properties Limited | Real estate | 2017 |
| JOE Energy Limited | Wholesale & retail | 2018 |
| JOE Holdings Limited | Financial services | 2023 |
| JOF Consultants Limited | Professional & scientific | 2017 |
| Joffin's (London) Ltd | Arts & recreation | 2001 |
| Jogis Group Limited | Administrative & support | 2022 |
| JOH Couriers Ltd | Transport & logistics | 2025 |
| Johananoop Limited | Health & social care | 2018 |
| John Amos & Co. Ltd. | Professional & scientific | 2006 |
| John Amos Rural Ltd. | Real estate | 2006 |
| John Dandy Motors Limited | Wholesale & retail | 2000 |
| John DIX Travel Ltd | Transport & logistics | 2015 |
| John King Conservatories & Windows Limited | Construction | 2002 |
| John Matthews Contracting Ltd | Construction | 2013 |
| John Mckenzie Autoservices Limited | Wholesale & retail | 2025 |
| John's Private Hire Cars Ltd | Other services | 2013 |
| JOHN@2020 Ltd | Health & social care | 2022 |
| Johnnie Catering Salutions Ltd | Transport & logistics | 2019 |
| Johns Property Investments Ltd | Real estate | 2019 |
| Joinhome Property Management Limited | Professional & scientific | 1990 |
| Jojin & Hema Limited | Real estate | 2024 |
| Joluke Consulting Services Limited | Professional & scientific | 2011 |
| JON Luckett Ltd | Construction | 2017 |
| Jones Business Consulting Ltd | Financial services | 2024 |
| Jones E-Commerce Ltd | Wholesale & retail | 2025 |
| Jones Electrical Solutions Ltd | Construction | 2024 |
| Jones Homes (Gloucester) Limited | Real estate | 2024 |
| Jones Plant Hire Ltd | Administrative & support | 2014 |
| Jones Plant Sales Ltd | Wholesale & retail | 2015 |
| Jones Thornton Publishing & Communications Limited | Information & communication | 2014 |
| Jonny Rocks Ltd | Transport & logistics | 2015 |
| Jooc Ltd | Wholesale & retail | 2018 |
| Jordan & SON Developments Ltd | Real estate | 2025 |
| Jordan Craft Limited | Information & communication | 2020 |
| Jordan H Ltd | Professional & scientific | 2024 |
| Jordan White Carpentry Limited | Construction | 2018 |
| Joremi Homes Ltd | Real estate | 2025 |
| Joseph's Tdpa Ltd | Administrative & support | 2020 |
| Journey in Style Limited | Administrative & support | 2022 |
| Jovi Associates Ltd | Real estate | 2021 |
| Joy Tea Group Limited | Manufacturing | 2026 |
| JP Home Services Limited | Administrative & support | 2026 |
| JPL Property Holdings Ltd | Real estate | 2026 |
| JPM Transport Ltd | Transport & logistics | 2014 |
| JPS Electrical & Fire Ltd | Construction | 2023 |
| JPS Renewable Contracting Ltd | Construction | 2020 |
| JPS UK Holdings Ltd | Real estate | 2024 |
| Jpws Plumbing Limited | Construction | 2022 |
| JR Angle LE Ltd | Real estate | 2025 |
| JRB Elite Ltd | Construction | 2019 |
| Jrdn Ltd | Professional & scientific | 2022 |
| JRG Transport (South West) Limited | Transport & logistics | 2014 |
| Jrhs Music Limited | Information & communication | 2017 |
| JRK Property Solutions Ltd | Administrative & support | 2024 |
| Jrmade Solutions Limited | Construction | 2024 |
| JRP Mortgages Ltd | Financial services | 2024 |
| JS Architectural Services Ltd | Professional & scientific | 2019 |
| JS FM Consultancy Services Limited | Administrative & support | 2025 |
| JS Glos Ltd | Construction | 2021 |
| JSE Real Estate Limited | Real estate | 2021 |
| JSG Pubs & Bars Ltd | Hospitality | 2025 |
| JSK Birmingham Ltd | Wholesale & retail | 2015 |
| JSM (UK) Ltd | Manufacturing | 2014 |
| JSM Business Gifts Ltd | Wholesale & retail | 2014 |
| JSM Innovations Group Ltd | Financial services | 2022 |
| JSN Brothers Ltd | Real estate | 2022 |
| JST Asset Holdings Ltd | Financial services | 2023 |
| JST Kitchens (Kesseler Cheltenham) Ltd | Wholesale & retail | 2023 |
| JTD Technical Services Ltd | Real estate | 2020 |
| JTR Accountancy Ltd | Professional & scientific | 2025 |
| Juba J Barbers Ltd | Other services | 2025 |
| Jubilee Court (Bibury) Management Company Limited | Households | 2005 |
| Judges Lodgings Gloucester Ltd | Real estate | 2019 |
| Judges Lodgings Ltd | Real estate | 2019 |
| Juice Data Limited | Information & communication | 2003 |
| Juicy Studios Ltd | Professional & scientific | 2022 |
| Julian Rogers Auto Services Ltd | Wholesale & retail | 2023 |
| Jumer Properties Ltd | Real estate | 2020 |