| J George Caravans Limited | Other services | 2017 |
| J George Developments Limited | Real estate | 2011 |
| J George Limited | Real estate | 2013 |
| J Green Plumbing & Heating Ltd | Construction | 2022 |
| J J Plant Hire (Glos) Limited | Construction | 2004 |
| J Jelf Building Limited | Construction | 2024 |
| J L E Autos Ltd | Wholesale & retail | 2012 |
| J M Wellman Consultancy Ltd | Professional & scientific | 2023 |
| J Mann Building and Letting Limited | Real estate | 2021 |
| J Martin Equine Services Ltd | Professional & scientific | 2007 |
| J Matthews Landscapes Limited | Construction | 2021 |
| J P a S Contracting Ltd | Administrative & support | 2024 |
| J P Vitalcare Ltd | Health & social care | 2026 |
| J Paterson Electrical Ltd | Construction | 2009 |
| J Payne Ltd | Construction | 2023 |
| J R Building Ltd | Construction | 2003 |
| J Royles Skip Hire Limited | Administrative & support | 2009 |
| J S Gill Limited | Transport & logistics | 2015 |
| J S Weygang & Partners | | 1991 |
| J Tamblyn Limited | Administrative & support | 2013 |
| J Telecommunications Ltd | Wholesale & retail | 2025 |
| J W Ortho Limited | Administrative & support | 1998 |
| J Warner Contracting Limited | Administrative & support | 2002 |
| J Williams Contracting Services Ltd | Construction | 2025 |
| J Wingate Electrical Services Ltd | Construction | 2026 |
| J Young Fuels Limited | Wholesale & retail | 2011 |
| J&J Property Development (Gloucester) Ltd | Real estate | 2023 |
| J&K Property Holdings Limited | Real estate | 2018 |
| J&L Consultants Ltd | Professional & scientific | 2024 |
| J&P Solutions Ltd | Manufacturing | 2025 |
| J&R Singh Logistics Ltd | Transport & logistics | 2026 |
| J&S Autocare Ltd | Wholesale & retail | 2020 |
| J&S Property Trading Ltd | Real estate | 2016 |
| J.A. Green Electrical Ltd | Construction | 2025 |
| J.A.K.K. Security & Facilities Management Limited | Administrative & support | 2018 |
| J.B.Electrical Contracting Services Ltd | Construction | 2019 |
| J.C. Aldridge & SON Limited | Construction | 1975 |
| J.C. Lloyd Building Contractors Limited | Construction | 2012 |
| J.D. Carpentry & Building Ltd | Construction | 2015 |
| J.E Estate Maintenance Limited | Administrative & support | 2019 |
| J.H.C. Incorporated Limited | Real estate | 2006 |
| J.Jireh Consultants Ltd | Information & communication | 2022 |
| J.P.W. (Floorings) Limited | Construction | 1978 |
| J.R. Heating Ltd | Construction | 2005 |
| J.T.H. Engineering Limited | Manufacturing | 1984 |
| J.W.Housden Limited | Construction | 1964 |
| J.W.S. Ltd. | Construction | 1974 |
| J17 Limited | Professional & scientific | 2022 |
| J2 Electrical Contractors Ltd | Construction | 2006 |
| J2 Property Management Ltd | Real estate | 2021 |
| J3 Carpentry Ltd | Manufacturing | 2022 |
| J7 Vehicle Solutions Limited | Wholesale & retail | 2024 |
| Jaalne-Quaerere Ltd | Financial services | 2013 |
| Jaanu Luxe Ltd | Real estate | 2025 |
| Jacalan Management Ltd | Real estate | 2022 |
| Jack & Akhil’S Autos Ltd | Wholesale & retail | 2024 |
| Jack and Alice Homes Ltd | Real estate | 2023 |
| Jack Hill Culture Company Ltd | Wholesale & retail | 2014 |
| Jack Lowman Ltd | Professional & scientific | 2017 |
| Jack Pinky Limited | Professional & scientific | 2018 |
| Jackie Marshall-Cyrus & Associates Limited | Other services | 2016 |
| Jackman Marketing Consultants Limited | Professional & scientific | 2007 |
| Jackoval Ltd | Wholesale & retail | 2023 |
| Jacks Office Ltd | Information & communication | 2020 |
| Jackson Builders & Property Maintenance Ltd | Construction | 2017 |
| Jade Hammond Ltd | Arts & recreation | 2018 |
| Jade Roselli Cosmetologist Ltd | Other services | 2022 |
| Jade Wilce Ltd | Administrative & support | 2025 |
| Jaffagate Retail Ltd | Wholesale & retail | 2023 |
| Jaffer Developments Ltd | Real estate | 2015 |
| JAG Plumbing and Heating Limited | Construction | 2021 |
| Jaguar Barber Limited | Other services | 2023 |
| Jake Pryszlak Consultancy Ltd | Professional & scientific | 2024 |
| Jake Spira Ltd | Extraterritorial | 2021 |
| Jakemans (Confectioners) Limited | Manufacturing | 1938 |
| Jakk Security Gloucester Limited | Administrative & support | 2025 |
| Jaks Excel Ltd | Real estate | 2026 |
| Jalaram News Cheltenham Ltd | Wholesale & retail | 2006 |
| JAM Design & Build Ltd | Construction | 2017 |
| Jamcodirect Limited | Other services | 2016 |
| Jame Properties Ltd | Real estate | 2014 |
| James and Morgan Property Limited | Households | 2023 |
| James Barry & Sons Limited | Professional & scientific | 2017 |
| James Bubb Landscaping Ltd | Administrative & support | 2021 |
| James Daryll COX Ltd | Education | 2023 |
| James Farquharson Limited | Professional & scientific | 1991 |
| James Howkins Limited | Construction | 2026 |
| James Milne Limited | Health & social care | 2011 |
| James Timms Transport Limited | Transport & logistics | 1978 |
| James&Sons 2018 Ltd | Administrative & support | 2018 |
| Jamie Dean Plumbing & Heating Limited | Construction | 2020 |
| JAN - ALF Cheltenham Limited | Extraterritorial | 2017 |
| Janbil Limited | Wholesale & retail | 2025 |
| Jane BEE Safeguarding Ltd | Professional & scientific | 2017 |
| Jane Messenger Limited | Professional & scientific | 2013 |
| Janes Bakery Limited | Manufacturing | 1971 |
| Janine.Me Ltd | Wholesale & retail | 2025 |
| Jaon Limited | Information & communication | 2025 |
| Jare Hooper Holdings Ltd | Financial services | 2025 |
| Jarrodean Personnel Ltd | Administrative & support | 2012 |