| Glevum Structural Design Limited | Professional & scientific | 2019 |
| Glevum Trade Ltd | Wholesale & retail | 2014 |
| Gliu Ltd | Professional & scientific | 2020 |
| Glo-Active CIC | Health & social care | 2011 |
| Global Blueprint Limited | Professional & scientific | 2008 |
| Global Design Depot Limited | Professional & scientific | 2025 |
| Global Hills Interior Decoration Company Limited | Construction | 2017 |
| Global Property Maintenance Limited | Wholesale & retail | 2016 |
| Global Satisfaction Limited | Wholesale & retail | 2024 |
| Global Sporting Venues Ltd | Arts & recreation | 2020 |
| Globalexceltech Limited | Information & communication | 2023 |
| Globel Software Ltd | Administrative & support | 2016 |
| Globexia Ltd | Wholesale & retail | 2026 |
| Glos Events Company Limited | Administrative & support | 2023 |
| Glos Food Delight Ltd | Hospitality | 2025 |
| Glos Property Services Ltd | Real estate | 2023 |
| Glos Technology Limited | Information & communication | 2025 |
| Glosfarm Ltd | Wholesale & retail | 2014 |
| Glosgo Transport Ltd | Transport & logistics | 2025 |
| Glosit Ltd | Wholesale & retail | 2014 |
| Glosjobs Limited | Administrative & support | 2015 |
| Glosproperties Ltd | Real estate | 2025 |
| Gloss Facilities Management Ltd | Transport & logistics | 2025 |
| Gloster CNC Repairs Limited | Manufacturing | 2012 |
| Gloster Company Limited | Wholesale & retail | 2022 |
| Gloster Tech Solutions Ltd | Information & communication | 2013 |
| Gloster Tooling Supplies Limited | Wholesale & retail | 2010 |
| Glostrans Ltd | Transport & logistics | 2017 |
| Glosweld Construction Limited | Construction | 2015 |
| Glouce Homes Ltd | Real estate | 2025 |
| Gloucester Air Conditioning Ltd | Energy | 2025 |
| Gloucester ALL Blues Rugby Football Club | Arts & recreation | 2008 |
| Gloucester and District Irish Society Limited (the) | Hospitality | 1974 |
| Gloucester Antiques Centre Limited | Wholesale & retail | 2016 |
| Gloucester Asbestos Ltd | Water & waste | 2009 |
| Gloucester Barber Limited | Other services | 2023 |
| Gloucester Beds Centre Ltd | Wholesale & retail | 2021 |
| Gloucester BID Limited | Administrative & support | 2017 |
| Gloucester Bike Specialist Ltd | Administrative & support | 2010 |
| Gloucester Boathouse Limited | Arts & recreation | 2012 |
| Gloucester Boiler Services Ltd | Construction | 2017 |
| Gloucester Brewery Group Limited | Manufacturing | 2019 |
| Gloucester Brewery Ltd | Manufacturing | 2011 |
| Gloucester Builders Ltd | Construction | 2025 |
| Gloucester Businesses CIC | Other services | 2023 |
| Gloucester Cable Recycling Ltd | Administrative & support | 2022 |
| Gloucester Carpet Cleaning Limited | Administrative & support | 2023 |
| Gloucester Carpet Outlet Ltd | Wholesale & retail | 2013 |
| Gloucester Cars Limited | Transport & logistics | 2018 |
| Gloucester Catering Services Limited | Hospitality | 1989 |
| Gloucester Cathedral Enterprises Limited | Wholesale & retail | 1967 |
| Gloucester Cattle Society Limited | Agriculture & fishing | 2009 |
| Gloucester Church Building CIC | Financial services | 2025 |
| Gloucester City AFC Foundation C.I.C. | Health & social care | 2018 |
| Gloucester City AFC Limited | Arts & recreation | 2021 |
| Gloucester City Association Football Club (1980) Limited | Arts & recreation | 1980 |
| Gloucester City Football Club Limited | Extraterritorial | 1999 |
| Gloucester City Ltd. | Extraterritorial | 2000 |
| Gloucester City Mission | Health & social care | 2006 |
| Gloucester City Swimming Club Limited | Arts & recreation | 2015 |
| Gloucester Civic Trust Limited | Other services | 1972 |
| Gloucester Classic Cars Ltd | Wholesale & retail | 2023 |
| Gloucester Cleaning Services Ltd | Administrative & support | 2025 |
| Gloucester Commercial Space Limited | Real estate | 2024 |
| Gloucester Community Building Collective C.I.C. | Other services | 2019 |
| Gloucester Computer Services Limited | Other services | 2003 |
| Gloucester Contemporary Artists CIC | Arts & recreation | 2024 |
| Gloucester Couriers Limited | Transport & logistics | 2021 |
| Gloucester Dental Care Limited | Health & social care | 2020 |
| Gloucester Dental Specialists Limited | Health & social care | 2021 |
| Gloucester Diocesan Board of Finance | Other services | 1919 |
| Gloucester Diocesan Trust | Other services | 1910 |
| Gloucester Drainage Ltd | Water & waste | 2025 |
| Gloucester Drylining Ltd | Construction | 2020 |
| Gloucester Eastgate Ltd | Hospitality | 2026 |
| Gloucester Electrical Contractors Ltd | Construction | 2011 |
| Gloucester Electrical Ltd | Construction | 2021 |
| Gloucester Engineering Supplies Limited | Wholesale & retail | 2002 |
| Gloucester Event Hire Ltd | Administrative & support | 2012 |
| Gloucester Event Medics | Health & social care | 2004 |
| Gloucester Express Ltd | Wholesale & retail | 2026 |
| Gloucester Family Care Ltd | Health & social care | 2020 |
| Gloucester Farmers Club Limited | Hospitality | 2009 |
| Gloucester Feed the Hungry CIC | Hospitality | 2022 |
| Gloucester Fight Factory Limited | Arts & recreation | 2019 |
| Gloucester Fish & Chips Ltd | Hospitality | 2025 |
| Gloucester FM C.I.C. | Information & communication | 2004 |
| Gloucester Food Dock Limited | Real estate | 2008 |
| Gloucester Foodbank | Health & social care | 2005 |
| Gloucester Framing and Art Limited | Wholesale & retail | 2019 |
| Gloucester Freight Services Limited | Transport & logistics | 1987 |
| Gloucester Fusion Ltd | Hospitality | 2025 |
| Gloucester Garden Design Company Limited | Administrative & support | 2024 |
| Gloucester GAS Services Limited | Construction | 2021 |
| Gloucester Grill GG Ltd | Hospitality | 2023 |
| Gloucester Grill Ltd | Hospitality | 2020 |
| Gloucester Hartpury Rugby Ltd | Arts & recreation | 2023 |
| Gloucester Heating & Plumbing Limited | Real estate | 2020 |
| Gloucester Home and Garden Services Limited | Construction | 2024 |
| Gloucester Homecare Services Ltd | Health & social care | 2011 |