| Wheatway Services Limited | Manufacturing | 2006 |
| Wheatway Solutions Limited | Manufacturing | 2008 |
| Wheel Master Tech Ltd | Manufacturing | 2017 |
| WHEEL2WHEEL Ltd | Administrative & support | 2023 |
| Whiles Tuition Limited | Education | 2021 |
| Whilst WE ARE Here CIC | Professional & scientific | 2025 |
| Whisked Away Ltd | Hospitality | 2025 |
| Whispercroft Limited | Professional & scientific | 2004 |
| White Box Group Holdings Limited | Financial services | 2020 |
| White Box Interiors Limited | Construction | 2016 |
| White Box Living Ltd | Real estate | 2020 |
| White Cat Investments Limited | Administrative & support | 2014 |
| White END Marketing Limited | Administrative & support | 2024 |
| White Light Hosting Limited | Professional & scientific | 2011 |
| White Pigeons Production Ltd | Information & communication | 2025 |
| White Room Images Limited | Professional & scientific | 2009 |
| Whitewalk 2013 Limited | Administrative & support | 2013 |
| Whitminster Trading Company Limited | Wholesale & retail | 1998 |
| Whitson Consulting Ltd | Information & communication | 2013 |
| Whittle Bloom Limited | Hospitality | 2023 |
| Whitton CMS Limited | Construction | 2016 |
| Whitwolf Limited | Hospitality | 2023 |
| Whitworth Court Management Company Limited | Administrative & support | 2005 |
| Whole MAN Academy Ltd | Education | 2020 |
| Wholesale Fixing Ltd | Other services | 2022 |
| Wholesale Mobile Tech Ltd | Wholesale & retail | 2025 |
| Wholly Gelato Ltd | Manufacturing | 2022 |
| Wicasa Limited | Information & communication | 2008 |
| Wichard Manley Properties Limited | Real estate | 2024 |
| Widow Retreats Ltd | Hospitality | 2023 |
| Wigglebottoms Ltd | Arts & recreation | 2024 |
| Wightfield Manor Limited | Households | 1989 |
| Wigosam Limited | Real estate | 2026 |
| Wigs Ltd | Wholesale & retail | 2025 |
| Wilco Consultancy and Research Services Limited | Professional & scientific | 2021 |
| Wilcox Drainage Ltd | Construction | 2020 |
| Wild & Green Outdoors CIC | Education | 2026 |
| Wild Saas Limited | Wholesale & retail | 2025 |
| Wildbuck Media Ltd | Professional & scientific | 2025 |
| Wilder Glos Limited | Professional & scientific | 2018 |
| Wilderness Natural Ltd | Agriculture & fishing | 2023 |
| Wildfire Global Holdings Limited | Real estate | 2013 |
| Wildfire Systems Limited | Wholesale & retail | 2004 |
| Wildfowl & Wetlands Trust (Consulting) Limited | Professional & scientific | 1988 |
| Wildmoor Restaurants Ltd | Hospitality | 2026 |
| Wilfred Independent Ltd | Professional & scientific | 2013 |
| Will Lloyd-Baker Limited | Hospitality | 2021 |
| William DAW Close Management Company Limited | Extraterritorial | 2026 |
| William DE LA More Limited | Wholesale & retail | 2009 |
| William I'Anson Holdings Limited | Financial services | 2023 |
| William I'Anson Limited | Wholesale & retail | 2010 |
| William Morrison (Cheltenham) Ltd | Construction | 2015 |
| William Paige Ltd | Real estate | 2008 |
| William T Nicholls Limited | | 1918 |
| Williams Door Systems (WDS) Limited | Manufacturing | 2026 |
| Willingdon Construction Ltd | Construction | 2019 |
| Willow Sporting Group Ltd | Arts & recreation | 2023 |
| Willow Wellness Care Ltd | Health & social care | 2026 |
| Willowberry Skincare Ltd | Manufacturing | 2017 |
| Willowstone Property Holdings Ltd | Real estate | 2025 |
| Wilson Cook Commercial Ltd | Financial services | 2021 |
| Wilson Studios Photography Ltd | Professional & scientific | 2026 |
| Winch Productions Limited | Wholesale & retail | 2024 |
| Winchcombe Sports Hub Limited | Arts & recreation | 2011 |
| Window Machinery Solutions Ltd | Wholesale & retail | 2026 |
| Window Manufacturing Solutions Ltd | Manufacturing | 2024 |
| Window Pains Stroud Limited | Construction | 2018 |
| Window Widgets Limited | Wholesale & retail | 2000 |
| Windsmead Consultancy Ltd | Information & communication | 2011 |
| Windswept and Groovy Limited | Information & communication | 2025 |
| Wing Hing Gloucester Ltd | Hospitality | 2017 |
| Winsor Vincent Limited | Professional & scientific | 2012 |
| Winston Little Ltd | Information & communication | 2010 |
| Winters & Cleaver Brickwork Limited | Construction | 2014 |
| Winters Management Consultants Ltd | Professional & scientific | 2022 |
| Wise ME UP Limited | Professional & scientific | 2019 |
| Wisefoothealth Ltd | Health & social care | 2025 |
| Wiseman Commercial Consultants Limited | Professional & scientific | 2020 |
| Wiseman Consulting Limited | Administrative & support | 2013 |
| Wisla Supermarket South Ltd | Wholesale & retail | 2014 |
| With Whimsical Intent Ltd | Wholesale & retail | 2024 |
| Withydale Estate Limited | Transport & logistics | 2011 |
| Witts Properties Ltd | Real estate | 2018 |
| Wizard Lifetime Limited | Financial services | 2022 |
| WJM Electrical (Glos) Ltd | Construction | 2022 |
| WKI Investments Limited | Financial services | 2024 |
| WMB Corporate Finance Limited | Financial services | 2023 |
| WMG Group Ltd | Financial services | 2016 |
| WMS South West Ltd | Water & waste | 2024 |
| WNQ Ltd | Wholesale & retail | 2022 |
| WOD Weekends Ltd | Administrative & support | 2020 |
| Wodland Fitness Ltd | Arts & recreation | 2018 |
| WOK on Flame Ltd | Hospitality | 2018 |
| WOK to Walk Bold Street Limited | Hospitality | 2016 |
| Woksbax Limited | Hospitality | 2023 |
| Wold & Vale Tree Consultancy Limited | Professional & scientific | 2020 |
| Wolf Assets Limited | Financial services | 2020 |
| Wolf Recruitment Group Franchises Ltd | Education | 2020 |
| Women's Tower of Strength C.I.C. | Hospitality | 2023 |
| WON Studio Ltd | Wholesale & retail | 2022 |