| Unite Trading Ltd | Wholesale & retail | 2022 |
| Unitecom Ltd | Information & communication | 2021 |
| United Medical Group Limited | Financial services | 2018 |
| Unity High-Performance Coaching Ltd. | Education | 2025 |
| Unity Property Limited | Real estate | 1982 |
| Univation Partners Ltd | Professional & scientific | 2026 |
| Universal IT Function Ltd | Information & communication | 2016 |
| Universal Training and Transport Limited | Education | 2007 |
| Universel Ltd | Administrative & support | 2019 |
| Unlimit Digital Ltd | Information & communication | 2025 |
| Unlimit LAB Limited | Information & communication | 2025 |
| Unlimited Properties Limited | Extraterritorial | 1971 |
| Upholstery Techniques (Winchcombe) Limited | Manufacturing | 2019 |
| Upton Builders & Sons Limited | Construction | 2019 |
| Upton Coffee House Limited | Hospitality | 2023 |
| Upton Equestrian Ltd | Wholesale & retail | 2019 |
| Upton ST Leonards Life Community Interest Company | Information & communication | 2014 |
| Upton's Garden Management Company Limited | Households | 2003 |
| Upturn Trading Ltd | Wholesale & retail | 2024 |
| UR Accountants Limited | Professional & scientific | 2015 |
| Urban Martial Arts School (Gloucester) Ltd | Arts & recreation | 2022 |
| Urban Martial Arts Schools - Forest of Dean Limited | Arts & recreation | 2023 |
| USA Nails Fashion Ltd | Other services | 2024 |
| USL Village Store Ltd | Wholesale & retail | 2026 |
| Utilities & Infrastructure Limited | Extraterritorial | 2007 |
| Utility Customer Service Management Ltd | Professional & scientific | 2005 |
| Utopia Accountants Limited | Professional & scientific | 2020 |
| Utopia Childrens Services Limited | Health & social care | 2009 |
| Utopianknight Consultancy Limited | Information & communication | 2025 |
| Utreatibill Limited | Financial services | 2026 |
| V & C Maintenance (Glos) Limited | Public administration | 2024 |
| V & C Ventilation Ltd | Manufacturing | 2002 |
| V and V Cars Ltd | Wholesale & retail | 2025 |
| V R Engineering Services Limited | Professional & scientific | 2015 |
| V. W. Derrington Limited | Manufacturing | 2014 |
| V.R. Properties (Glos) Ltd | Hospitality | 2006 |
| V2CARE Ltd | Administrative & support | 2021 |
| Vajk Limited | Real estate | 2018 |
| Valets 2 U Specialists Ltd | Administrative & support | 2022 |
| Valets 4 U Specialists Ltd | Administrative & support | 2021 |
| Valiy Limited | Transport & logistics | 2015 |
| Valkyrie Support Services Ltd | Education | 2005 |
| Valley Pest Control Limited | Administrative & support | 2002 |
| Valleys Court RTM Company Limited | Households | 2017 |
| Valorae Ltd | Information & communication | 2025 |
| Value Home and Travel Ltd | Wholesale & retail | 2026 |
| Value Tyres.Co.Uk Limited | Extraterritorial | 2004 |
| Valve Repair Solutions & Consulting Limited | Professional & scientific | 2026 |
| Vaneco Ltd | Wholesale & retail | 2024 |
| Vanguard Fight Labs Ltd | Manufacturing | 2025 |
| Vanguard Healthcare Solutions (Modular) Limited | Manufacturing | 2023 |
| Vanguard Healthcare Solutions Limited | Health & social care | 2002 |
| Vanguard MSP Limited | Information & communication | 2023 |
| Vanishing Inc. UK Limited | Arts & recreation | 2015 |
| Vantage Yacht Supplies Ltd | Manufacturing | 2019 |
| Vape IT Easy Limited | Wholesale & retail | 2015 |
| Varadius Ltd | Information & communication | 2016 |
| Vardar Holdings Limited | Financial services | 2023 |
| Vardar Systems Limited | Professional & scientific | 2010 |
| VAS Hand Car Wash Ltd | Wholesale & retail | 2020 |
| VAS Kebab Ltd | Hospitality | 2026 |
| Vasi Delivery 24 Ltd | Transport & logistics | 2023 |
| Vault AI Ltd | Information & communication | 2025 |
| VC Executive Coaching Ltd | Professional & scientific | 2019 |
| VC Property Developments Ltd | Real estate | 2026 |
| Vcentral Ltd | Information & communication | 2006 |
| Veblen Wines Ltd | Administrative & support | 2008 |
| Vedika Engineering Limited | Professional & scientific | 2019 |
| Veek Limited | Wholesale & retail | 2011 |
| Veestra Ltd | Professional & scientific | 2026 |
| Veil & Valour Tattoo Ltd | Other services | 2026 |
| Velarsafe FM Ltd | Administrative & support | 2025 |
| Velvet Green Properties Ltd | Real estate | 2025 |
| Velvet Tree Studio's Ltd | Professional & scientific | 2015 |
| Vendetta Archive Limited | Wholesale & retail | 2022 |
| Vendetta Studios Limited | Wholesale & retail | 2026 |
| Venn's Lane Limited | Health & social care | 2020 |
| Vent Port Solutions Limited | Construction | 2015 |
| Venture Electrical Services Limited | Construction | 2006 |
| Venturer Construction Limited | Construction | 2011 |
| Verdaxis Ltd | Professional & scientific | 2025 |
| Verge High Access Ltd | Construction | 2026 |
| Vergo Pest Management Ltd | Administrative & support | 1996 |
| Veritable Solutions Limited | Information & communication | 2006 |
| Verity Cleaning and Recruiting Company Ltd | Administrative & support | 2022 |
| Vero Active Ltd | Wholesale & retail | 2025 |
| Veroshield Limited | Professional & scientific | 2026 |
| Versabuild South West Ltd | Construction | 2025 |
| Versatile Controls Holdings Limited | Financial services | 2026 |
| Versatile Controls Limited | Wholesale & retail | 1977 |
| Versys Property Ltd | Real estate | 2025 |
| Vertue Group Limited | Administrative & support | 2026 |
| Verve Property Group Limited | Hospitality | 2021 |
| Verve Property Partnership Group LLP | | 2021 |
| Vestum UK Limited | Financial services | 2022 |
| VET DEV Limited | Professional & scientific | 2011 |
| Veto Pest Ltd | Administrative & support | 2026 |
| Vetri Global PVT Ltd | Wholesale & retail | 2025 |
| Vetsglobal Ltd | Information & communication | 2011 |
| VIA Lucis Consulting Ltd | Administrative & support | 2020 |