| Aura by Freya Limited | Wholesale & retail | 2025 |
| Auralia Solutions Limited | Information & communication | 2013 |
| Aurevix Ltd | Manufacturing | 2025 |
| Aurora - Hair & Piercing Ltd | Other services | 2024 |
| Aurora ASD Limited | Education | 2016 |
| Aurora Care and Education Holdings Limited | Education | 2015 |
| Aurora Care and Education Midco Limited | Education | 2015 |
| Aurora Care and Education Opco Limited | Education | 2015 |
| Aurora Dawn Limited | Health & social care | 2016 |
| Aurora FE Limited | Education | 2016 |
| Aurums Ceylon Ltd | Wholesale & retail | 2024 |
| Auseklis Limited | Professional & scientific | 2013 |
| Autarkeia Care Services Ltd | Health & social care | 2023 |
| Authentic Intelligence Global Ltd | Administrative & support | 2013 |
| Auto Air Gloucester Ltd | Wholesale & retail | 2002 |
| Auto Economy Limited | Wholesale & retail | 2013 |
| Auto Fleet Ltd | Administrative & support | 1999 |
| Auto Parts Online Ltd | Wholesale & retail | 2019 |
| Auto Tyre & Battery Co Limited | Professional & scientific | 2003 |
| Auto-Mech Services Ltd | Wholesale & retail | 2010 |
| Automann Ltd | Wholesale & retail | 2019 |
| Automated Operation Services Ltd | Manufacturing | 2022 |
| Automotive and Computer Electronics Ltd | Wholesale & retail | 2025 |
| Automotive Trade Ltd | Wholesale & retail | 2019 |
| Autonex Moto Limited | Wholesale & retail | 2025 |
| Autopave Ltd | Administrative & support | 2026 |
| Autotrans (Gloucester) Limited | Wholesale & retail | 2007 |
| Autovalue 2000 Limited | Transport & logistics | 2008 |
| AUTOVALUE5 Limited | Wholesale & retail | 2016 |
| Autovision Windscreens Limited | Wholesale & retail | 2015 |
| AV Carpentry Ltd | Construction | 2023 |
| AV1 Building Limited | Construction | 2020 |
| Avalana Ventures Ltd | Financial services | 2025 |
| Avalonia Medical Services Ltd | Health & social care | 2019 |
| Avantech Lighting Ltd | Wholesale & retail | 2015 |
| Avantguard Security Limited | Administrative & support | 2009 |
| Avanti-Emg Ltd | Wholesale & retail | 2001 |
| Avantis Americas Limited | Information & communication | 2014 |
| Avantis Apac Limited | Manufacturing | 2015 |
| Avantis Education Limited | Information & communication | 2007 |
| Avantis Group Ltd | Information & communication | 2015 |
| Avenue Care Limited | Health & social care | 2023 |
| Avenue Cars of Gloucester 2020 Limited | Wholesale & retail | 2020 |
| Avenue Cars of Gloucester Limited | Wholesale & retail | 1988 |
| AVI Wavey Ltd | Arts & recreation | 2025 |
| Aviator Crew Limited | Health & social care | 2013 |
| Avib Ltd | Transport & logistics | 2018 |
| Avislim Ltd | Other services | 2019 |
| Avon and Severn Drain Services Ltd | Extraterritorial | 2011 |
| Avon Dental Care Ltd | Health & social care | 2010 |
| Avon Properties (2025 ) Limited | Real estate | 2025 |
| Avon Specialty Metals Limited | Water & waste | 2012 |
| Avon Valley Real Estate Ltd | Real estate | 2024 |
| Avro Electric Vehicles Ltd | Extraterritorial | 2022 |
| AW Tree Surgery and Garden Care Ltd | Administrative & support | 2021 |
| Awaycationholidays Ltd | Hospitality | 2015 |
| AWB Accountancy and Taxation Services Limited | Professional & scientific | 2012 |
| Awesomepainter Ltd | Construction | 2025 |
| AWJ Accounting Ltd | Professional & scientific | 2024 |
| Awl.Building.Services Limited | Construction | 2015 |
| Axcess Energy Limited | Professional & scientific | 2010 |
| AXE Realty Limited | Real estate | 2025 |
| Axiom Building Services Ltd | Construction | 2006 |
| Axiom UK Holdings Limited | Financial services | 2020 |
| Axis Building Services Limited | Construction | 2017 |
| Axium Labs Limited | Information & communication | 2025 |
| Ayam Transport Limited | Transport & logistics | 2025 |
| AYO Live in Care & Supported Living Ltd | Health & social care | 2023 |
| Ayrton Road (Churchdown) Management Company Limited | Extraterritorial | 2025 |
| AZ Financial Solutions Ltd | Information & communication | 2019 |
| AZ Food GLO Ltd | Hospitality | 2025 |
| AZ Links Ltd | Information & communication | 2024 |
| Azhar Associates Ltd | Health & social care | 2025 |
| Azunwatech Ltd | Information & communication | 2025 |
| Azure Investment Management Ltd | Real estate | 2023 |
| AZY South Ltd | Wholesale & retail | 2025 |
| B & Co Stow Ltd | Wholesale & retail | 2023 |
| B & G Properties (INV) Ltd | Real estate | 2019 |
| B & J Haulage Services Limited | Transport & logistics | 1995 |
| B & R Blinds Ltd | Other services | 2019 |
| B Creations Ltd | Manufacturing | 2020 |
| B F Sweepers Limited | Administrative & support | 2011 |
| B G Brain Decorators Limited | Construction | 2010 |
| B Grade Domestics Limited | Wholesale & retail | 2000 |
| B Handlowiec Ltd | Wholesale & retail | 2024 |
| B K Jones Properties Ltd | Construction | 2012 |
| B Mortgage Services Limited | Financial services | 2020 |
| B Safe Driving School Ltd | Education | 2025 |
| B. & R. Engineering (Glos) Limited | Professional & scientific | 2003 |
| B. & W. Engineering (Gloucester) Limited | Construction | 1975 |
| B.A.M. (Gloucester Estates) Limited | Real estate | 1972 |
| B.B. Enterprises (Contractors Gloucester) Limited | Construction | 1965 |
| B.F Electrical Ltd | Manufacturing | 2019 |
| B.F.S Engineering Limited | Professional & scientific | 1999 |
| B.Hopper Advisory Ltd | Financial services | 2022 |
| B.I.T.S. Limited | Professional & scientific | 1986 |
| B.J. Kennels & Cattery Limited | Other services | 2010 |
| B.M.A. Residential Limited | Real estate | 2021 |
| B.P. & S.C. Limited | Manufacturing | 1979 |
| B.P.S. Holdings Limited | Manufacturing | 2003 |