| A & a Livestock Limited | Agriculture & fishing | 2024 |
| A & E Howat Limited | Energy | 2018 |
| Adventure Centre for Education (Ace) | Education | 2010 |
| Affective Rail UK Ltd | Transport & logistics | 2025 |
| AG Vending Solutions Limited | Other services | 2020 |
| Ailsa Craig Gifts Limited | Manufacturing | 2021 |
| Ailsa Craig Gifts Maidens Limited | Professional & scientific | 2011 |
| Ailsa Craig Limited | Extraterritorial | 2020 |
| Ailsa Finance Limited | Financial services | 2025 |
| Alan Jeans Veterinary Services Limited | Professional & scientific | 2003 |
| Alex Paton & SON (Livestock) Ltd | Agriculture & fishing | 2021 |
| Amber Inns (Girvan) Ltd | Other services | 2023 |
| Ardmillan Business Consultancy Ltd | Professional & scientific | 2012 |
| Ashanti Groundworks Ltd | Administrative & support | 2022 |
| ATB Accountancy Services Limited | Professional & scientific | 2002 |
| Austin's Restaurant Limited | Hospitality | 2017 |
| Auto Consult International Limited | Wholesale & retail | 2016 |
| Avasey Homes Limited | Real estate | 2024 |
| Ayrshire LOG Cabins Limited | Wholesale & retail | 2007 |
| Ballantrae Rural Initiative Care in the Community Limited | Health & social care | 1998 |
| Ballywhiskin Holiday Park Ltd | Hospitality | 2020 |
| Baltor Ltd | Mining & quarrying | 2012 |
| Baluntonhill Limited | Energy | 1997 |
| Bardrochat Estate Management Company Limited | Professional & scientific | 2015 |
| Barr Community Shop and Cafe Ltd | Administrative & support | 2012 |
| Barrhill Community Interest Company | Financial services | 2008 |
| Basmati Blue Ltd | Hospitality | 2019 |
| Bazz Products Ltd | Wholesale & retail | 2023 |
| Bennane Shore Holiday Park Ltd | Hospitality | 2017 |
| Beyond Innovation Limited | Professional & scientific | 2008 |
| Beyside Limited | Energy | 2017 |
| Billymc Ltd | Administrative & support | 2016 |
| Biosphere Bikes | Manufacturing | 2018 |
| Birnam Securities (Glasgow) Limited | Real estate | 1973 |
| Blairside Limited | Energy | 2023 |
| BLP 2004-58 Limited | Real estate | 2004 |
| Bontoc Homes Limited | Real estate | 2003 |
| Brandedleys Holiday Park Ltd | Hospitality | 2012 |
| Breezenodes Ltd | Information & communication | 2024 |
| Bridgemillco Ltd | Real estate | 2021 |
| Broadholm Energy Ltd | Energy | 2022 |
| Bruach Property Ltd | Real estate | 2010 |
| Burnlea Properties Limited | Real estate | 2013 |
| Cairnhill Holdings Limited | Wholesale & retail | 2020 |
| Campbell Land Management Ltd | Administrative & support | 2018 |
| Camregan Renewables Limited | Energy | 2011 |
| Carleton Farming Limited | Agriculture & fishing | 2006 |
| Carrick Futures | Administrative & support | 2009 |
| Charge Electric Ltd | Manufacturing | 2019 |
| Chirmorrie Enterprises Ltd. | Agriculture & fishing | 1999 |
| Coif Limited | Financial services | 2011 |
| Coif Properties Limited | Real estate | 2012 |
| Combined Inns (Commercial Inn) Ltd | Hospitality | 2016 |
| COMPTECH42 Ltd | Professional & scientific | 2024 |
| Croft (Scotland) Limited | Wholesale & retail | 2013 |
| Crossing Properties Ltd. | Real estate | 1996 |
| CTC Property Solutions Limited | Real estate | 2018 |
| Cumloden Nursing Home Limited | Health & social care | 1988 |
| D Horne Services Limited | Administrative & support | 2015 |
| D M Multimedia Limited | Information & communication | 1999 |
| Dailly Community Activity Centre Association Limited | Arts & recreation | 2010 |
| Daldowie Farm Ltd | Agriculture & fishing | 2020 |
| Dalquharran Limited | Agriculture & fishing | 2019 |
| DAMLA34 Ltd | Hospitality | 2026 |
| Dams of Craigie Farm Limited | Agriculture & fishing | 1970 |
| Davrail UK Ltd | Transport & logistics | 2025 |
| Diamond CUT Refurb Wheels Ltd | Manufacturing | 2024 |
| Donanside Limited | Energy | 2023 |
| Drumlochart Holiday Park Ltd | Hospitality | 2016 |
| Dunnymuck Farm | | 1988 |
| Dunrannie Co Ltd | Real estate | 2021 |
| Elsay-May Cabins Limited | Hospitality | 2018 |
| Energybodylife Ltd | Health & social care | 2026 |
| Engineering Inclusion Ltd | Professional & scientific | 2023 |
| EV Innovation Ltd | Construction | 2017 |
| Exhibit Scotland Limited | Information & communication | 2017 |
| Farmery Haulage Ltd | Transport & logistics | 2023 |
| Flaunt and Flourish Ltd | Professional & scientific | 2021 |
| Floss and Bond Ltd | Health & social care | 2021 |
| Flytor Ltd | Financial services | 2018 |
| FM Components Ltd | Manufacturing | 2019 |
| FM Global Limited | Manufacturing | 2025 |
| Forefindsgolfballs Ltd | Wholesale & retail | 2025 |
| Forestry & Recycling Equipment Ltd | Wholesale & retail | 2019 |
| Fountainhead Cottages Limited | Hospitality | 2021 |
| Geraldine Kelly Limited | Administrative & support | 2024 |
| Girvan Accident Repair Centre Limited | Wholesale & retail | 2020 |
| Girvan Community Garden | Health & social care | 2009 |
| Girvan Consulting Ltd | Professional & scientific | 2023 |
| Girvan Fast Food Ltd | Hospitality | 2023 |
| Girvan Outreach & Hub CIC | Health & social care | 2024 |
| Girvan Rentals Ltd | Households | 2025 |
| Girvan Spicy Food Ltd | Hospitality | 2024 |
| Girvan Store Ltd | Wholesale & retail | 2025 |
| Girvan Youth Trust | Administrative & support | 1999 |
| Glenapp Estate Company Limited | Agriculture & fishing | 1963 |
| Glenapp Farms Limited | Agriculture & fishing | 1981 |
| Glisk Studio Ltd | Information & communication | 2025 |
| Glow Fuels Limited | Energy | 2017 |
| Golf Recreation Scotland Limited | Financial services | 2014 |