| 1963 Lighting Limited | Construction | 2021 |
| A E Arthur Orthopaedics Ltd | Health & social care | 2025 |
| AAA Fabrication (Scotland) Ltd | Construction | 2022 |
| AB21 Joinery & Building Ltd | Construction | 2021 |
| AB21 Timber Frames Ltd | Construction | 2024 |
| Aesthetics by Nurse AMY Ltd | Other services | 2026 |
| AHV Renewables Ltd | Professional & scientific | 2026 |
| AJG Group Holdings Limited | Real estate | 2024 |
| AJG Group Limited | Information & communication | 2018 |
| ALL Comms Group Limited | Administrative & support | 2024 |
| Alpha Janitorial Supplies Limited | Hospitality | 2016 |
| AP Housing and Support Ltd | Health & social care | 2023 |
| Ardeer Research and Marketing Limited | Administrative & support | 1998 |
| Aroma at the Mill Ltd | Hospitality | 2023 |
| Arran View Holiday Park Ltd | Hospitality | 2019 |
| Assault Courses UK Ltd | Arts & recreation | 2014 |
| Ayrshire Recovery Limited | Wholesale & retail | 2023 |
| Banging Binz & Gardens Ltd | Water & waste | 2022 |
| Barwood Leisure Limited | Hospitality | 2017 |
| Beard Armour Limited | Manufacturing | 2017 |
| BGS House One Limited | Households | 2025 |
| Bigtinylittle Limited | Other services | 2018 |
| Bike Tuff Ltd | Wholesale & retail | 2025 |
| Buzz Global Services Ltd | Professional & scientific | 2018 |
| C&S Property Services Limited | Real estate | 2018 |
| Cairn View Properties Ltd | Real estate | 2026 |
| Calder Gordon Maintenance Ltd | Administrative & support | 2023 |
| Cameron Core Limited | Professional & scientific | 2025 |
| Castle Foods Ltd | Extraterritorial | 2008 |
| CJM Access & Coatings Ltd | Construction | 2024 |
| Cleanslateext Ltd | Administrative & support | 2025 |
| CM Rail Contracts Ltd | Construction | 2020 |
| Colin Mcquade Limited | Construction | 2014 |
| Commpare Utilities Ltd | Administrative & support | 2025 |
| Confidence Scotland Installations Ltd | Construction | 2016 |
| Contract QS Ltd | Professional & scientific | 2015 |
| Cowans Law Limited | Agriculture & fishing | 2025 |
| Craigie Mains Dairy Ltd | Agriculture & fishing | 2024 |
| Crestpoint Professional Services Ltd | Construction | 2025 |
| Cycle Station Bike Hire Limited | Manufacturing | 2019 |
| Dales Solicitors LLP | | 2012 |
| Details Music Ltd | Other services | 2026 |
| Dickie Industries Ltd | Arts & recreation | 2023 |
| Dynamo Construct Limited | Construction | 2016 |
| EJB Contracts Ltd | Professional & scientific | 2025 |
| Ellden Group Ltd | Construction | 2024 |
| Elysian Professional Ltd | Wholesale & retail | 2025 |
| Ember Energy Ltd | Administrative & support | 2016 |
| Enercare Scotland Ltd | Construction | 2016 |
| Engage Fitness Ltd | Arts & recreation | 2017 |
| Fallco Limited | Administrative & support | 2014 |
| FCV (Scotland) Ltd | Construction | 2015 |
| Four One Safety Solutions Ltd | Professional & scientific | 2017 |
| G&C Ecommerce Group Ltd | Wholesale & retail | 2023 |
| Galahad Software Limited | Information & communication | 2025 |
| Gateside Stables Limited | Agriculture & fishing | 2023 |
| Gather Retail Ltd | Wholesale & retail | 2025 |
| GD Heating & Plumbing Services Ltd | Construction | 2021 |
| GD Touring Ltd | Arts & recreation | 2026 |
| Ginger Racing Ltd | Arts & recreation | 2023 |
| Glasgow Couriers Ltd | Transport & logistics | 2024 |
| Grant Brothers Limited Liability Partnership | | 2009 |
| Grants Direct Limited | Wholesale & retail | 2020 |
| Grants Foods (Scotland) Limited | Extraterritorial | 2002 |
| H and L Energy Limited | Administrative & support | 2022 |
| Henderson & Calder Limited | Hospitality | 2024 |
| Hoose on the Hill Ltd | Hospitality | 2021 |
| IC Mobile LAB Ltd | Information & communication | 2011 |
| ICR Innovative Solutions Ltd | Professional & scientific | 2020 |
| Inglis & Co Limited | Professional & scientific | 2022 |
| J a Landscapes Ltd | Administrative & support | 2022 |
| James a. Gibson & Co. (Cowgrove) Limited | Agriculture & fishing | 1957 |
| James Gibb (Country Feeds) Limited | Wholesale & retail | 1987 |
| JBY Pubs Limited | Hospitality | 2018 |
| JET Tech OPS Ltd | Transport & logistics | 2018 |
| JEW BOY Ltd | Wholesale & retail | 2026 |
| JM ALL Trades Ayrshire Ltd | Professional & scientific | 2026 |
| Jomac Property Ltd | Real estate | 2020 |
| JWA Pipeworks Ltd | Manufacturing | 2025 |
| KBF Consult Ltd | Professional & scientific | 2024 |
| KFX Properties Ltd | Households | 2021 |
| Kilmarnock Tyres Limited | Wholesale & retail | 2012 |
| Klairvo Holdings Ltd. | Financial services | 2025 |
| L Foster Accountancy Services Ltd | Professional & scientific | 2022 |
| LA Spine & Neurosurgery Limited | Health & social care | 2015 |
| Ladyton Ltd | Agriculture & fishing | 2021 |
| Ladyton Manor Ltd | Agriculture & fishing | 2021 |
| LGS Home Improvements Ltd | Construction | 2010 |
| LMC Machining Ltd | Manufacturing | 2024 |
| Loudoun Books Ltd | Wholesale & retail | 2011 |
| M&H Driveways Ltd | Construction | 2026 |
| M&M Investments Ayrshire Limited | Real estate | 2016 |
| Macchaluim Holdings Group Ltd | Financial services | 2025 |
| Malhi Kitchen Ltd | Hospitality | 2023 |
| Marmaris Galston Ltd | Hospitality | 2013 |
| Marriott Property Management Limited | Real estate | 2020 |
| Mccrone Contracts Ltd. | Administrative & support | 2013 |
| Medical Finance Limited | Professional & scientific | 2013 |
| MGS Automotive & Rally Limited | Wholesale & retail | 2016 |
| ML Water Solutions Limited | Administrative & support | 2018 |