| Stow Cycle Hub CIC | Other services | 2021 |
| Suntan Tanning Beauty and Nails Ltd | Other services | 2024 |
| Swanston Consultancy Services Limited | Professional & scientific | 2017 |
| SWT Confectionery Limited | Wholesale & retail | 2025 |
| T M Contracting (Airdrie) Limited | Transport & logistics | 2016 |
| T R F Enterprises | | 1983 |
| T.D. Renwick (Peel) | | 1991 |
| Tahoe Properties Ltd | Real estate | 2025 |
| Take to the Water CIC | Health & social care | 2022 |
| Tandom Limited | Health & social care | 2023 |
| Tapdrop Limited | Education | 1995 |
| Taradale Limited | Real estate | 2010 |
| Tartan + Tweed Ltd | Wholesale & retail | 2012 |
| TD Consultants Ltd | Professional & scientific | 2025 |
| Tekfit Ltd | Arts & recreation | 2018 |
| Tembro Joinery Limited | Construction | 2010 |
| Tempest Brewing Co Limited | Manufacturing | 2013 |
| THE Artbox (Borders) Ltd | Arts & recreation | 2018 |
| THE Borders Citizens Advice Consortium | Other services | 2012 |
| THE Borders Menopause Clinic Ltd | Health & social care | 2022 |
| THE Coffee Tree (Galashiels) Ltd | Hospitality | 2020 |
| THE Edge Scotland Ltd. | Extraterritorial | 2011 |
| THE Fleece Bar and Kitchen Limited | Hospitality | 2018 |
| THE Furniture Company (Galashiels) Limited | Wholesale & retail | 2017 |
| THE Heartland Market CIC | Wholesale & retail | 2022 |
| THE Kebab Shop 1 Limited | Hospitality | 2024 |
| THE Ostle Property Group Ltd | Real estate | 2020 |
| THE Paper Chain (Scotland) Ltd. | Manufacturing | 2000 |
| THE Plough Inn Leitholm Limited | Hospitality | 2014 |
| THE Scott Street Partnership | | 2002 |
| THE Spa Plan Ltd. | Wholesale & retail | 2015 |
| THE Tree Wise MEN Limited | Agriculture & fishing | 2002 |
| THE Trophy GUY Limited | Wholesale & retail | 2007 |
| THE Vintage Besoms Ltd | Wholesale & retail | 2025 |
| THE WEE Scotty Cleaners Limited | Other services | 2023 |
| THE Wild Outdoors Childcare Ltd | Health & social care | 2022 |
| THE Wildings Distillery Limited | Manufacturing | 2024 |
| THE Xmod Ltd | Wholesale & retail | 2007 |
| THE Yoga Mill Ltd | Arts & recreation | 2017 |
| Thehyreco Cafe Ltd | Hospitality | 2025 |
| Thehyreco Furniture Ltd | Wholesale & retail | 2025 |
| Thermadisc Ltd | Manufacturing | 2025 |
| Thermo-Glaze (Borders) Limited | Construction | 2010 |
| Thirlestane Investments Ltd | Real estate | 2025 |
| Thistle Public Affairs Ltd | Professional & scientific | 2026 |
| Thistle Rocketry Ltd | Manufacturing | 2019 |
| Thomas Hogben Film Productions Limited | Information & communication | 2021 |
| Thornfield House Selkirk Limited | Health & social care | 2020 |
| Thornylee Farms | | 1986 |
| Thyme in the Garden Ltd | Administrative & support | 2023 |
| Tide & Thyme Ltd | Hospitality | 2021 |
| Tile Style (Borders) Limited | Wholesale & retail | 2011 |
| Timberlodge Limited | Administrative & support | 1998 |
| TJM Hospitality Ltd | Hospitality | 2018 |
| TLL Borders Ltd | Other services | 2025 |
| Tofss Ltd | Hospitality | 2023 |
| TOM Hurley Ltd | Manufacturing | 2026 |
| Torin Management Ltd | Professional & scientific | 2026 |
| Trade-Mob Limited | Wholesale & retail | 2022 |
| Triacordia | Other services | 2023 |
| Trimontium Management Company Limited | Real estate | 2009 |
| Triple R UK Ltd | Administrative & support | 2009 |
| TS Williamson Builder & Landscaper Limited | Construction | 2021 |
| Tubbecke Group Ltd | Construction | 2022 |
| Tula & Cooper Ltd | Arts & recreation | 2022 |
| TVK Cementing Oil&Gas Ltd | Mining & quarrying | 2026 |
| Tweed Chiropractic Limited | Health & social care | 2012 |
| Tweed Valley Foods Limited | Hospitality | 2016 |
| Tweed Valley Storage Limited | Transport & logistics | 2023 |
| Tweedvale Ltd | Professional & scientific | 2024 |
| Tweedview Properties Ltd | Real estate | 2023 |
| Umbra Lash Ltd | Wholesale & retail | 2026 |
| Unique Selling Mart Ltd | Wholesale & retail | 2025 |
| Universal Ceilings & Partitions Limited | Construction | 2010 |
| UTE Limited | Real estate | 2024 |
| V & V Accessories Ltd | Wholesale & retail | 2020 |
| Valve Brewing Company Ltd. | Manufacturing | 2015 |
| Vanman Movers Ltd | Transport & logistics | 2025 |
| Vault of Curiosities Limited | Wholesale & retail | 2015 |
| VK7 Services Limited | Professional & scientific | 2018 |
| VTF Inspections Ltd | Professional & scientific | 2026 |
| W D Mundell & SON | | 1983 |
| W. & D. Allan Limited | Agriculture & fishing | 1965 |
| Waffles N Brew Ltd | Hospitality | 2021 |
| Waffles N Chill Ltd | Hospitality | 2025 |
| Waterhead Properties Limited | Real estate | 2010 |
| Weir Property Investments Limited | Real estate | 2005 |
| Wheatlands Storage Ltd | Transport & logistics | 2025 |
| Wild Leaf Reels C.I.C. | Professional & scientific | 2016 |
| Wild Rovers - Rooftop Camping and Vintage Vehicles Limited | Professional & scientific | 2012 |
| WILDERHAUGH001 Limited | Professional & scientific | 2019 |
| Wildfire Spirit Limited | Wholesale & retail | 2021 |
| Wilson Builders and Joiners Ltd. | Construction | 2013 |
| Wilson G. Jamieson Limited | Real estate | 2001 |
| Winter Spring Summer & Fall Ltd | Wholesale & retail | 2021 |
| With a Cherry on Top Scotland Ltd | Hospitality | 2021 |
| Wok&Barr Ltd | Hospitality | 2025 |
| World Class Barbers Ltd | Other services | 2019 |
| WSZ Joiners Limited | Construction | 2012 |
| Y & S Limited | Wholesale & retail | 2009 |