| 1273 Taxis Ltd. | Professional & scientific | 2007 |
| 17 High Street Limited | Wholesale & retail | 2025 |
| 1ST for Jobs & Access Limited | Construction | 2023 |
| 7A Solutions Ltd | Manufacturing | 2026 |
| A & a Doyle Limited | Other services | 2011 |
| A & J Elliot Electrical Limited | Manufacturing | 2009 |
| A.C Civils & Concrete Ltd | Construction | 2025 |
| A.C Roads & Driveways Ltd | Construction | 2019 |
| A7 Car-Wash Ltd | Administrative & support | 2026 |
| Abbeyhall Homes Ltd | Extraterritorial | 2021 |
| Abbotsford Homes Limited | Construction | 2001 |
| Abbotsford Plumbing & Heating Limited | Construction | 2011 |
| Abeerah Ltd | Hospitality | 2014 |
| Abstraction Studio Ltd | Arts & recreation | 2025 |
| ACA Investments Ltd | Real estate | 2024 |
| ACC Vehicle Installation Solutions Limited | Wholesale & retail | 2012 |
| Accountwing Ltd | Professional & scientific | 2025 |
| AD Ignotus Ltd | Wholesale & retail | 2024 |
| Adam Purves Estates Ltd | Real estate | 2015 |
| Adam Purves Retail Limited | Wholesale & retail | 2023 |
| Aero Leather Clothing Ltd. | Manufacturing | 1990 |
| AET Services Ltd | Professional & scientific | 2018 |
| Afina Stay Limited | Hospitality | 2025 |
| Ainslie Contracting Limited | Other services | 2008 |
| Ainslie Mclean Joinery Limited | Construction | 2022 |
| Air King Solutions Ltd | Construction | 2025 |
| Air Supply Ventilation Ltd | Energy | 2014 |
| Airtech Ventures Limited | Manufacturing | 2020 |
| AJ Facilities Ltd | Administrative & support | 2023 |
| AJW89 Limited | Administrative & support | 2016 |
| AKB Developments Limited | Hospitality | 2013 |
| Akita Leisure Ltd | Hospitality | 2026 |
| AL Fareed Store Ltd | Agriculture & fishing | 2025 |
| ALA Property Solutions Ltd | Real estate | 2023 |
| Alborado Limited | Real estate | 2003 |
| Alex Dalgetty & Sons Limited | Manufacturing | 2010 |
| Alifin Foods Limited | Hospitality | 2024 |
| Alistair Corcoran (Builder) Ltd | Construction | 2009 |
| ALL Joinery Ltd | Construction | 2025 |
| Altonis Investments Ltd | Professional & scientific | 2021 |
| AMS Associates Limited | Professional & scientific | 2012 |
| Andrew Bird Consulting Ltd | Professional & scientific | 2010 |
| Andrew Holmes Glass Limited | Administrative & support | 2016 |
| Andy Reeves Projects Ltd | Professional & scientific | 2020 |
| Angus Architecture Limited | Professional & scientific | 2021 |
| Anne Cranston Limited | Agriculture & fishing | 2010 |
| Annie's Cafe Limited | Hospitality | 2011 |
| Annies Cafe Galashiels Ltd | Hospitality | 2024 |
| Ansa People Ltd | Administrative & support | 2024 |
| Anthony Phillips Consulting Limited | Agriculture & fishing | 2025 |
| APE Holdings Limited | Real estate | 2015 |
| Apgl Holdings 2023 Limited | Financial services | 2023 |
| Apgl Holdings Limited | Wholesale & retail | 2015 |
| Apis Carpentry & Joinery Ltd | Construction | 2025 |
| Arkitech Ltd | Professional & scientific | 2016 |
| Art of DYE Ltd | Wholesale & retail | 2022 |
| Artisan Route Limited | Wholesale & retail | 2015 |
| Atina Properties Limited | Real estate | 2018 |
| Audax Commercial Property (UK) Ltd | Construction | 2024 |
| Audax Consultancy Services (UK) Ltd | Professional & scientific | 2024 |
| Audax Enterprises (UK) Ltd | Wholesale & retail | 2023 |
| Audax Holdings (UK) Limited | Financial services | 2024 |
| Auld Alliance Trading Company Limited | Wholesale & retail | 2008 |
| Autovalet Car & Van Sales Ltd | Wholesale & retail | 2020 |
| AZAZEL80 Ltd | Hospitality | 2021 |
| B L Services Limited | Construction | 2015 |
| Baguette Express Franchising Limited | Hospitality | 2006 |
| Balnakeil Bay Ltd | Real estate | 2023 |
| Barnetts Catering Limited | Hospitality | 2010 |
| Barony Castle Hotel Limited | Hospitality | 2023 |
| Barsfield Services Limited | Professional & scientific | 2022 |
| Bathroom Planet (Borders) Limited | Construction | 2008 |
| Bcomrie. Ltd | Construction | 2017 |
| Bella Aesthetics Limited | Health & social care | 2018 |
| Benchmark Motor Company Limited | Wholesale & retail | 2011 |
| BH Fixings Online Ltd | Wholesale & retail | 2025 |
| BID for Galashiels Limited | Other services | 2025 |
| Big Bear Electrics Ltd | Construction | 2025 |
| Big Voices Musical Theatre Company CIC | Arts & recreation | 2024 |
| Bikescape Ltd. | Transport & logistics | 2015 |
| Bill Jeffrey Promotions Limited | Administrative & support | 2023 |
| Biorationale Limited | Professional & scientific | 2014 |
| Black Swan Strategies Ltd | Information & communication | 2025 |
| Blackhorse Cafe & Bistro Limited | Hospitality | 2020 |
| Blainslie Firewood Company Limited | Manufacturing | 2017 |
| Boletus Edulis Ltd | Hospitality | 2021 |
| Bonnie Barista (Scotland) Ltd | Hospitality | 2024 |
| Border ALL Trades Ltd | Construction | 2019 |
| Border BED Company Limited | Wholesale & retail | 2015 |
| Border Bingo Clubs Limited | Arts & recreation | 1999 |
| Border Cleaning Fairies Ltd | Administrative & support | 2020 |
| Border Computing Limited | Wholesale & retail | 1995 |
| Border Construction (Holdings) Limited | Construction | 2000 |
| Border Domestics Ltd | Other services | 2023 |
| Border Electrical & Property Maintenance Ltd | Construction | 2025 |
| Border Physiotherapy Clinic Ltd | Health & social care | 2014 |
| Border Timberguard Limited | Construction | 2008 |
| Borders Beauty Studio Ltd | Other services | 2020 |
| Borders Carpets Ltd | Wholesale & retail | 2026 |
| Borders Community Radio & Media Limited | Information & communication | 2012 |