| Simon Lewis Agencies Limited | Real estate | 1994 |
| Single Parents on Holiday Ltd | Administrative & support | 2005 |
| Sirius Star Property Limited | Real estate | 2021 |
| SK Baylis House Slough Ltd | Hospitality | 2022 |
| SK Hotels Limited | Hospitality | 2017 |
| Skinda Limited | Wholesale & retail | 2022 |
| SKS Security Systems Limited | Administrative & support | 2002 |
| Sky Sentinel Ltd | Extraterritorial | 2010 |
| Snobby Doggy Limited | Extraterritorial | 2022 |
| Sofia's Little Loves Limited | Wholesale & retail | 2026 |
| Sophie North Casting Ltd | Administrative & support | 2011 |
| South Coast Preservation Ltd | Construction | 1991 |
| Spaceship DOT Earth Limited | Wholesale & retail | 2013 |
| Speldhurst Management Limited | Real estate | 2015 |
| Spice Hart Limited | Hospitality | 2022 |
| Spiral Properties Limited | Construction | 2020 |
| Spirit of Hemp Ltd. | Wholesale & retail | 2017 |
| Sports Cars GB Limited | Wholesale & retail | 2003 |
| Starting Over Show Ltd | Administrative & support | 2006 |
| Stonepark Drive Residents Association Limited | Households | 1986 |
| Studio E G Limited | Professional & scientific | 2013 |
| Sussex Lifts Limited | Construction | 2006 |
| Sussex Prestige Ltd | Wholesale & retail | 2024 |
| Svendsen Consulting Ltd | Professional & scientific | 2025 |
| Swan Close Management Company Limited | Households | 1991 |
| Swanvale Limited | Hospitality | 1991 |
| Swites Limited | Agriculture & fishing | 1997 |
| T J Williams Property Management Ltd | Real estate | 2016 |
| Tablehurst Farm Limited | Agriculture & fishing | 1996 |
| Talent Cloud Global Ltd | Information & communication | 2025 |
| Talent Property Services Ltd | Real estate | 2020 |
| Tarada Enterprises Ltd | Professional & scientific | 2025 |
| Tartanshore Media Films Ltd | Information & communication | 2021 |
| Team ZX1 (Services) Limited | Professional & scientific | 2014 |
| TEK Imports Limited | Wholesale & retail | 2023 |
| Temple Lodge Publishing Limited | Information & communication | 1993 |
| Terracorp (UK) Limited | Real estate | 2014 |
| Tersus Homes Ltd | Real estate | 2018 |
| Tessa the Saddler Limited | Wholesale & retail | 2021 |
| Tethys Investments Limited | Financial services | 2023 |
| Texaco Limited | Administrative & support | 2023 |
| TFW Property UK Ltd | Real estate | 2015 |
| THE Anderida Project Community Interest Company | Health & social care | 2022 |
| THE Ashdown Forest Foundation | Health & social care | 2018 |
| THE Bespoke Jacket Company Limited | Wholesale & retail | 2020 |
| THE Bield Forest ROW Limited | Households | 2025 |
| THE Christian Community Trustees Limited | Other services | 1987 |
| THE Community Art Studio CIC | Construction | 2025 |
| THE Crossing Community Supported Agriculture C.I.C. | Agriculture & fishing | 2017 |
| THE Design Gallery Limited | Wholesale & retail | 2002 |
| THE Driver's Drinks Company Limited | Manufacturing | 2018 |
| THE Event Academy Limited | Education | 2016 |
| THE Firefly Initiative CIC | Education | 2016 |
| THE Free Lotteries Ltd | Wholesale & retail | 2022 |
| THE Grand Hotel (Eastbourne) Limited | Hospitality | 1998 |
| THE Heighton Agency Limited | Administrative & support | 2020 |
| THE Inner Smile Mindfulness Organisation Limited | Administrative & support | 2023 |
| THE New INK Printing Company Limited | Manufacturing | 2006 |
| THE Oast Bakery Ltd | Manufacturing | 2022 |
| THE Oast Studio Ltd | Arts & recreation | 2024 |
| THE Resilience I Limited | Professional & scientific | 2016 |
| THE Shala School of Yoga Ltd | Education | 2026 |
| THE Shared Humanity Group Limited | Administrative & support | 2022 |
| THE Soul Millionaire Limited | Administrative & support | 2002 |
| THE User Research Centre Ltd | Administrative & support | 2020 |
| THE Wicks Battle Tea Rooms Limited | Hospitality | 2024 |
| Thedanes Ltd | Wholesale & retail | 2020 |
| Therapy Harley Street Limited | Health & social care | 2016 |
| Theyardkitchen Ltd | Hospitality | 2025 |
| Thirsty Business Limited | Health & social care | 2018 |
| Thirty Eight Limited | Administrative & support | 2017 |
| Thrive Global Limited | Professional & scientific | 2007 |
| Thyme Therapy Ltd | Health & social care | 2021 |
| Tidal Forest Limited | Hospitality | 2026 |
| Tina Kalivas Ltd | Information & communication | 2025 |
| Tobia Wolfe Fletcher Ltd | Real estate | 2021 |
| Tollo Academy Ltd | Education | 2021 |
| Tompty’S Cafe Ltd | Hospitality | 2025 |
| Tory Smith Limited | Hospitality | 2019 |
| Total Recalls Ltd | Information & communication | 2023 |
| Trendi Switch Ltd | Manufacturing | 2014 |
| Triglyph Books Ltd | Information & communication | 2019 |
| True Measure Solutions Limited | Construction | 2018 |
| Trueembed Sustainability Consulting Ltd | Professional & scientific | 2025 |
| Truppers Ltd | Manufacturing | 2022 |
| Tully De'Ath (Consultants) Ltd | Professional & scientific | 1990 |
| Tully De'Ath Limited | Administrative & support | 1985 |
| TWP Global Solutions Ltd | Transport & logistics | 2021 |
| Tylney Hall Hotel Limited | Hospitality | 1984 |
| UNIT3 Specialists Limited | Real estate | 1998 |
| V.G. Builders Limited | Construction | 1990 |
| Vanguard Motorsport Ltd | Arts & recreation | 2016 |
| Veasey & Sons Fishmongers Limited | Wholesale & retail | 2018 |
| Veasey & Sons LLP | | 2016 |
| Vessel and Sketch Ltd | Manufacturing | 2025 |
| Vince Taylor Tofts Ltd | Real estate | 2013 |
| Vinotech Solutions Limited | Wholesale & retail | 2026 |
| Viridis Organics Limited | Wholesale & retail | 2014 |
| Viscom Design Ltd | Professional & scientific | 2000 |
| Viva LA Diva Limited | Arts & recreation | 1998 |