| 5 D Property Services Limited | Construction | 2016 |
| A C Consultancy Limited | Professional & scientific | 2008 |
| Aardyl Ltd | Hospitality | 2022 |
| ABEBI1023 Ltd | Administrative & support | 2023 |
| Accounting and Taxation Solutions Limited | Professional & scientific | 2009 |
| Accurate Flat Roofing Limited | Construction | 2008 |
| Adept Physiotherapy and Healthcare Limited | Public administration | 2015 |
| ALI Barber Flitwick Ltd | Other services | 2024 |
| Amcomp Limited | Information & communication | 2011 |
| Amstrong International Ltd | Wholesale & retail | 2023 |
| Andosite Limited | Professional & scientific | 2015 |
| Anglo Carpentry Ltd | Construction | 2014 |
| AP Race (Holdings) Limited | Financial services | 2020 |
| AP Race (International) Limited | Education | 2024 |
| AP Swim Media Ltd | Information & communication | 2020 |
| Askmariehr Ltd | Professional & scientific | 2025 |
| Barking Finance Ltd | Professional & scientific | 2009 |
| Bedfordshire Bookkeeping Ltd | Professional & scientific | 2020 |
| Ben’S Bites Capital Management LLP | | 2023 |
| Bills' Car & Motorcycle Sales & Repairs Ltd | Wholesale & retail | 2020 |
| Black Panther (Investments) Ltd | Construction | 2014 |
| Blue Oak Interiors Limited | Construction | 2020 |
| Bravi Pro Ltd | Wholesale & retail | 2017 |
| Calele Limited | Administrative & support | 2016 |
| Caplong Ltd | Administrative & support | 2016 |
| Care IS Central Limited | Health & social care | 2020 |
| Care IS Central Residential Limited | Health & social care | 2023 |
| Cause WE CAN Ltd | Hospitality | 2019 |
| Caxton Healthcare Ltd | Administrative & support | 2024 |
| Cell-Tech (ST Albans) Limited | Construction | 2013 |
| Cipsi Taekwondo Ltd | Arts & recreation | 2018 |
| CL Management Solutions Limited | Professional & scientific | 2007 |
| Cleaning With Care Ltd | Administrative & support | 2020 |
| CLJ Driving Services Limited | Education | 2012 |
| Contracts Bathroom Supplies Ltd | Wholesale & retail | 2024 |
| Copenhagen Associates Ltd | Professional & scientific | 2015 |
| Corner View Ltd | Wholesale & retail | 2024 |
| Country Properties (Flitwick) Limited | Real estate | 1999 |
| Crafty Mish Mash Ltd | Arts & recreation | 2019 |
| Dabco Holdings Ltd | Professional & scientific | 2023 |
| Dandelion Transport Training Limited | Professional & scientific | 2014 |
| Declutter Limited | Professional & scientific | 2025 |
| Demaine Limited | Wholesale & retail | 2013 |
| Derek Doyle Light Haulage Ltd | Transport & logistics | 2019 |
| Drawers N Doors Ltd | Construction | 2016 |
| Essentials Digital Limited | Information & communication | 2021 |
| ETD Ltd | Health & social care | 2024 |
| Falconer Consultancy Services Ltd | Professional & scientific | 2016 |
| Flecks Drive, Wendy Management Company Limited | Real estate | 2024 |
| Flitwick & Ampthill Bathrooms Limited | Wholesale & retail | 2014 |
| Flitwick and Ampthill Lawn Tennis Club Limited(The) | Arts & recreation | 1956 |
| G J Ferry Limited | Professional & scientific | 2008 |
| Geopace Training Limited | Extraterritorial | 2019 |
| Ggats Estates Ltd | Real estate | 2022 |
| Global Response Media Ltd | Information & communication | 2018 |
| Grafton Cottages Ltd | Real estate | 2013 |
| Grant Palmer (Holdings) Limited | Financial services | 2023 |
| Grant Palmer Limited | Transport & logistics | 2010 |
| Green Badger Global Limited | Wholesale & retail | 2024 |
| Grumpybulldoggames Limited | Manufacturing | 2024 |
| House of Hydro Ltd | Other services | 2018 |
| HQ EFS Ltd | Construction | 2025 |
| Hurling Property Investment Limited | Real estate | 2017 |
| Ideal Eyewear Limited | Wholesale & retail | 2014 |
| Ikapoukis Ltd | Construction | 2017 |
| Infinity Air Conditioning Ltd | Energy | 2025 |
| Isis Internet Solutions Limited | Information & communication | 2004 |
| J & J Organics Limited | Wholesale & retail | 2024 |
| J.K Developments (Biggleswade) Ltd | Other services | 2013 |
| JDR Renovations Ltd | Construction | 2023 |
| JK Food Solutions Ltd. | Professional & scientific | 2019 |
| Jumper Network Systems Ltd | Construction | 2015 |
| Kantina Greek Street Food Ltd | Hospitality | 2020 |
| Kerry Ellis Productions Limited | Arts & recreation | 2022 |
| Kiln Motors Ltd | Wholesale & retail | 2026 |
| KPG Project Services Ltd | Professional & scientific | 2010 |
| Laparra Jewels Ltd | Manufacturing | 2015 |
| LG Mcintosh (UK) LLP | | 2026 |
| Limehouse Carpentry Limited | Construction | 2009 |
| LJ Electrical Contracting Ltd | Construction | 2025 |
| M & M Property Management Limited | Real estate | 1994 |
| M Parrott Consulting Limited | Professional & scientific | 2016 |
| M&M Property Estate Agents Limited | Real estate | 2011 |
| MAC Four Investments Limited | Financial services | 2023 |
| MAC1 Motors Ltd | Wholesale & retail | 2026 |
| Macroweb Limited | Manufacturing | 2004 |
| Maple Plastering Ltd | Construction | 2015 |
| Medigate Ltd | Manufacturing | 2023 |
| Mind Blmk | Health & social care | 1998 |
| Mookie Toys Limited | Professional & scientific | 2002 |
| Munday Door Services Ltd | Manufacturing | 2009 |
| Myross Site Engineering Limited | Construction | 2015 |
| Neodog Carpentry Limited | Construction | 2021 |
| Nonsequential Ltd | Professional & scientific | 2025 |
| Northwood Installations Limited | Manufacturing | 2022 |
| OG Rail Consultants Ltd | Construction | 2016 |
| Orchard Blinds & Shutters Ltd | Construction | 2022 |
| P and I Milligan Limited | Construction | 2024 |
| Pearl of Bengal Limited | Hospitality | 2010 |
| Perfect Ponds Solutions Limited | Construction | 2019 |