| Elyxa Group Limited | Administrative & support | 2023 |
| Elyxa Limited | Administrative & support | 2023 |
| Emancee Trading Limited | Professional & scientific | 2017 |
| Ember Labs Limited | Information & communication | 2025 |
| Embers Retreat Ltd | Health & social care | 2022 |
| Emberson & Co Limited | Real estate | 2004 |
| Embreo Ltd | Information & communication | 2010 |
| Emerging Generation Mission Ltd | Professional & scientific | 2026 |
| Emilia Romagna Limited | Hospitality | 2006 |
| Emmiga Ltd | Real estate | 2019 |
| Emms & Company Limited | Administrative & support | 1995 |
| Emotere Ltd | Professional & scientific | 2018 |
| EMP Editorial Ltd | Information & communication | 2025 |
| Empire Engineers Limited | Information & communication | 2015 |
| Empower at Scale Limited | Information & communication | 2025 |
| Empowermentcentric Ltd | Professional & scientific | 2022 |
| Empowermind Limited | Professional & scientific | 2020 |
| EMR Investments Limited | Real estate | 2015 |
| Encoeurage Limited | Professional & scientific | 2003 |
| Encuentro Ltd | Financial services | 2023 |
| Endeavour Aviation Ltd | Transport & logistics | 2019 |
| Endeavour Event Services Ltd | Administrative & support | 2015 |
| Endo MED Ltd | Health & social care | 2022 |
| Energy & Environmental Engineering Limited | Professional & scientific | 2010 |
| Energy and Infrastructure Management Limited | Professional & scientific | 2015 |
| Energy Efficiency Finance Limited | Professional & scientific | 2017 |
| Energy Finance Limited | Professional & scientific | 2017 |
| Energy Sports Physiotherapy Group Limited | Health & social care | 2022 |
| Energy Transact Investments (Italy) Limited | Administrative & support | 2017 |
| Energy Transact Investments Limited | Administrative & support | 2011 |
| Engco Developments Ltd | Construction | 2018 |
| Eniac Limited | Professional & scientific | 1999 |
| Enkindle Consulting Ltd | Financial services | 2025 |
| Enkindle Financial Planning Ltd | Financial services | 2025 |
| Entail Limited | Professional & scientific | 2016 |
| Enterprise Home Developments LLP | | 2012 |
| Enterprise Manor Park LLP | | 2012 |
| ENTERPRISE19 Limited | Wholesale & retail | 2021 |
| ENTERPRISE19 Support Services CIC | Wholesale & retail | 2017 |
| Entrinsic Consulting Ltd | Professional & scientific | 2025 |
| Enucleo Limited | Administrative & support | 2013 |
| Envisage Interiors Ltd | Wholesale & retail | 2022 |
| EOM Enterprises LLP | | 2010 |
| EOM Homes Limited | Construction | 2020 |
| Epic Play Ltd | Wholesale & retail | 2014 |
| Epona Thoroughbreds Ltd | Agriculture & fishing | 2021 |
| EPS Electrical Ltd | Construction | 2006 |
| Epsom Road (Management) Limited | Households | 2007 |
| Equal Talent Limited | Professional & scientific | 2017 |
| Equilibrium HR Services Limited | Administrative & support | 2012 |
| Equus Systems Limited | Information & communication | 2025 |
| Er-Ba Fish & Chips Ltd | Hospitality | 2026 |
| Ercolano (UK) Ltd | Wholesale & retail | 2011 |
| Ergosphere Software Limited | Information & communication | 2014 |
| Erity Limited | Information & communication | 2016 |
| Ernley Property Investment Co. Limited | Real estate | 1964 |
| ERP Commerce Limited | Professional & scientific | 2008 |
| Escolme Limited | Administrative & support | 2013 |
| ESL Cloud Solutions Limited | Information & communication | 2000 |
| ESL Distribution (Emea) Ltd | Information & communication | 2017 |
| ESL Distribution Limited | Information & communication | 2016 |
| ESL Professional Services Limited | Information & communication | 2014 |
| ESL Smart Solutions Limited | Extraterritorial | 2000 |
| ESL Space and Defence Solutions Ltd | Information & communication | 2018 |
| ESP Property Ltd. | Real estate | 2001 |
| Espan Digital Marketing Limited | Information & communication | 2011 |
| Espressa Management Limited | Real estate | 1999 |
| ETH Homes Ltd | Real estate | 2026 |
| Ethos Metrics Ltd | Professional & scientific | 2016 |
| Etive Pubs Limited | Hospitality | 2007 |
| ETR Holdings Limited | Wholesale & retail | 2024 |
| Eureka Digital Marketing Ltd | Information & communication | 2025 |
| Euro Medicity Ltd | Education | 2017 |
| Eurofin Services Limited | Professional & scientific | 1995 |
| Eurofin Taxation Services Limited | Professional & scientific | 2000 |
| Eurolux Consulting Ltd | Administrative & support | 2008 |
| Eurosoft Systems Limited | Information & communication | 1993 |
| EVA Organic Produce Ltd | Hospitality | 2022 |
| Eveia Occasions Ltd | Arts & recreation | 2025 |
| Eventsvalet.Co.Uk Ltd | Other services | 2020 |
| Eventus London Limited | Hospitality | 2017 |
| Evergreen PM Limited | Administrative & support | 2017 |
| Everything B3AUTIFUL Ltd. | Professional & scientific | 2018 |
| Everything LR Limited | Wholesale & retail | 2025 |
| Evidentis Ltd | Professional & scientific | 2026 |
| EVO Landscapes Ltd | Administrative & support | 2025 |
| Evodia Limited | Professional & scientific | 1997 |
| Evolution OT Ltd | Education | 2018 |
| Evolve Construction Ltd | Construction | 2024 |
| Evolve Leadership Coaching Ltd | Professional & scientific | 1997 |
| Evolve Neuropsychology Ltd | Health & social care | 2020 |
| Evolve Performing Arts Ltd | Arts & recreation | 2020 |
| Evoque Properties Limited | Real estate | 2005 |
| Evraz Surrey Ltd | Hospitality | 2024 |
| Ewens Inns Limited | Hospitality | 1997 |
| Ewer Creative Ltd | Administrative & support | 2023 |
| Ewshot Land Holdings Limited | Extraterritorial | 1990 |
| Exact Accounting Limited | Professional & scientific | 2005 |
| Exaptive Technologies Limited | Professional & scientific | 2025 |
| Excalibur Actuaries Limited | Financial services | 2017 |