| Industrial & Renewable Services Ltd | Construction | 2025 |
| Infinity Bathrooms Scotland Ltd | Construction | 2025 |
| Inglis Inns (Scotland) Ltd | Wholesale & retail | 2025 |
| Inishkea Limited | Manufacturing | 2010 |
| Inkreversal Ltd | Other services | 2026 |
| Innovative Environmental Services Ltd. | Water & waste | 2003 |
| Insight Immigration Ltd | Professional & scientific | 2025 |
| Inspect & Test UK Ltd | Manufacturing | 2020 |
| Inspired to Thrive Ltd | Financial services | 2017 |
| Interaktiv Design Studio Ltd | Information & communication | 2017 |
| Intuitive Assets Limited | Real estate | 2024 |
| Intuitive Interface Limited | Information & communication | 2017 |
| Inverness Mobile Podiatry Ltd | Other services | 2023 |
| Ionify Ltd | Construction | 2020 |
| IP Kebab House Ltd | Hospitality | 2018 |
| IPA Pool Ltd | Arts & recreation | 2024 |
| Ipek Properties Ltd | Real estate | 2021 |
| Irfan Textiles Limited | Wholesale & retail | 2025 |
| Iron House Scotland Ltd | Arts & recreation | 2024 |
| Ironworks Business Centre Limited | Real estate | 2020 |
| Irvine Consulting Services Limited | Professional & scientific | 2016 |
| Irvine HC Limited | Wholesale & retail | 2024 |
| Isla Murray Properties Limited | Real estate | 2025 |
| Itacg Ltd | Hospitality | 2025 |
| ITS Scot Ltd | Administrative & support | 2019 |
| Itta Falkirk Ltd | Hospitality | 2018 |
| Ivett Hirling Consultancy Ltd | Professional & scientific | 2022 |
| IZP Properties Limited | Real estate | 2023 |
| J & a Forestry Limited | Agriculture & fishing | 2018 |
| J & B Garage Services Ltd | Wholesale & retail | 2020 |
| J & J Holdings (Scotland) Limited | Financial services | 2021 |
| J & T Sons Limited | Wholesale & retail | 2017 |
| J &D Liqueur Limited | Hospitality | 2025 |
| J B Plastics & Containers Ltd | Manufacturing | 2025 |
| J Cairns Properties Ltd | Real estate | 2025 |
| J E M Engines Ltd. | Wholesale & retail | 2013 |
| J Harvey Roofing Limited | Construction | 2021 |
| J Hogg Ltd. | Professional & scientific | 2019 |
| J K Landscaping Limited | Administrative & support | 2017 |
| J P Homes Scotland Ltd | Real estate | 2024 |
| J Penns Couriers Ltd | Transport & logistics | 2019 |
| J Wood Developments Ltd | Construction | 2006 |
| J&K Tailoring Ltd | Professional & scientific | 2025 |
| J&T Westport Ltd | Hospitality | 2025 |
| J. Dunn & Sons Ltd | Construction | 2010 |
| J. E. Crozier Ltd. | Real estate | 2014 |
| J.F. Colley (Goldsmiths) Limited | Wholesale & retail | 2011 |
| J.F.L. Industrial Services Ltd | Professional & scientific | 2018 |
| J.L.P Group Limited | Water & waste | 2016 |
| J.Ng UK Consulting Limited | Manufacturing | 2022 |
| J33 (Holdings) Ltd | Financial services | 2026 |
| J33 Ltd | Transport & logistics | 2026 |
| J4 Joinery Ltd. | Other services | 2019 |
| JAA Associates Ltd | Information & communication | 2026 |
| Jaaplaw Limited | Professional & scientific | 2017 |
| JAC Inspection Solutions Ltd | Professional & scientific | 2023 |
| JAC15 Developments Ltd | Construction | 2018 |
| Jacal EOD Ltd | Professional & scientific | 2026 |
| Jack Walker Partnership Ltd | Manufacturing | 2005 |
| Jade's Sandwich Bar Limited | Hospitality | 2024 |
| Jahanpharm Limited | Health & social care | 2022 |
| JAM and T Ltd | Hospitality | 2020 |
| James Blair of Falkirk Limited | Wholesale & retail | 1976 |
| James Castwell Developments Limited | Construction | 2026 |
| James Davies (Scotland) Ltd. | Transport & logistics | 2002 |
| James HAY Memorials Limited | Other services | 1993 |
| James Horne of Limerigg Limited | Wholesale & retail | 1981 |
| James Murphy of Falkirk Limited | Manufacturing | 2001 |
| James Rose Homes (Alderston) Limited | Construction | 2024 |
| James Scott & Sons (Holdings) Limited | Administrative & support | 2014 |
| James Scott & Sons (Kitchen Equipment) Limited | Manufacturing | 1972 |
| James Sime and Company, Limited | Real estate | 1922 |
| Jamie Dick Drainage Ltd | Administrative & support | 2020 |
| Jannatgoods Limited | Wholesale & retail | 2023 |
| JAS & KAM Ltd | Real estate | 2024 |
| Java Bean Property Company Limited | Real estate | 2022 |
| Jaymodha Ltd | Real estate | 2022 |
| JB Gardens + Home Improvements Ltd | Administrative & support | 2026 |
| JB Mortgages Consultancy Ltd | Financial services | 2022 |
| JB Real Properties Ltd | Real estate | 2024 |
| JBR Roofing (Scotland) Limited | Construction | 2023 |
| JC Accountants Ltd | Professional & scientific | 2018 |
| JD Properties (Scotland) Ltd | Real estate | 2024 |
| JD Systems Integration Ltd. | Information & communication | 2005 |
| JDM Surfacing Limited | Construction | 2012 |
| Jedburgh Health Care Limited | Wholesale & retail | 2024 |
| Jennifer Kate Boutique Ltd | Wholesale & retail | 2022 |
| JF Health Holdings Ltd | Wholesale & retail | 2025 |
| JGW Enterprises Ltd | Wholesale & retail | 2025 |
| JHF Futures Ltd | Real estate | 2025 |
| Jilly Hair Extensions Limited | Other services | 2017 |
| JIM Jack Services Ltd. | Manufacturing | 2008 |
| JJ Azoo 105 Limited | Wholesale & retail | 2025 |
| JJ Optometry Ltd | Health & social care | 2010 |
| JL Lees Consulting Ltd | Information & communication | 2025 |
| JLC Retailer Ltd | Financial services | 2021 |
| JM NDT Inspection Limited | Professional & scientific | 2018 |
| JM Signature Estates Ltd | Real estate | 2025 |
| JMF Kane Property Ltd | Hospitality | 2023 |
| Jmk-Cad Ltd | Professional & scientific | 2023 |