| A. & K. Henry Limited | Construction | 2006 |
| A.J.B. Spence & SON Limited | Wholesale & retail | 2002 |
| Aitchisonropeaccess Ltd | Construction | 2026 |
| Aquamarine Charters Ltd | Transport & logistics | 2023 |
| Argyle Security Services Ltd | Administrative & support | 2024 |
| Bapville Limited | Professional & scientific | 2023 |
| Bapville Properties Limited | Real estate | 2025 |
| Beech Financial Solutions Limited | Financial services | 2011 |
| Bellevue Property Lets Ltd | Real estate | 2024 |
| Benjana Limited | Hospitality | 2018 |
| Berwick-Upon-Tweed Garden Centre Limited | Wholesale & retail | 1982 |
| BK Hospitality Ltd | Hospitality | 2020 |
| Bliss Beauty and Massage Limited | Other services | 2017 |
| Boat Investments Limited | Agriculture & fishing | 2012 |
| Bojangles Weddings Limited | Hospitality | 2017 |
| Borders Pottery C.I.C. | Arts & recreation | 2021 |
| Borders Roofing & Building Ltd | Construction | 2026 |
| Braw Buoys Limited | Arts & recreation | 2015 |
| Braw Buoys Theatre Company Limited | Arts & recreation | 2015 |
| Brian Collum and SON Ltd | Construction | 2026 |
| Bruce Gillie Joinery Ltd | Construction | 2025 |
| CAS PAR CAS Scotland Fish Ltd | Wholesale & retail | 2020 |
| Cash Kingdom Ltd | Arts & recreation | 2025 |
| Celtic Catering Ltd | Hospitality | 2024 |
| Colania Lodges Limited | Hospitality | 2014 |
| Coldingham Brave Bayers CIC | Administrative & support | 2023 |
| Coldingham Sands Community Company | Arts & recreation | 2008 |
| Conservatory Refurbishments Ltd | Administrative & support | 2021 |
| Conservatory Refurbishments Scotland Ltd | Construction | 2024 |
| Cubex Learning Ltd | Education | 2024 |
| D. R. Collin & SON Ltd | Manufacturing | 2010 |
| D.K. Access NDT Ltd | Professional & scientific | 2023 |
| D.R. Collin (Fish) Limited | Wholesale & retail | 2007 |
| D.R. Collin Scotland Limited | Wholesale & retail | 2011 |
| David Martin Funeral Directors Ltd | Other services | 2022 |
| DB Inquiry Solutions Ltd | Administrative & support | 2016 |
| DDP Cleaning Services Ltd | Administrative & support | 2023 |
| Dive Stay Limited | Arts & recreation | 2016 |
| Don'T Frown Aesthetics Ltd | Health & social care | 2025 |
| Douglas Nisbet Aviation Ltd | Transport & logistics | 2026 |
| Driven by Electric Limited | Professional & scientific | 2021 |
| East Reston Limited | Agriculture & fishing | 2003 |
| Edinburgh Executive Solutions Limited | Administrative & support | 2023 |
| Eyemouth Museum Trust Limited | Arts & recreation | 1981 |
| Eyestore Limited | Administrative & support | 2003 |
| Fahmar Limited | Hospitality | 2023 |
| Fork Truck Borders UK Instruction Limited | Transport & logistics | 2024 |
| Forthshore Enterprises Limited | Agriculture & fishing | 2003 |
| Gauche Pictures Ltd | Information & communication | 2023 |
| Geotechnical Investigation Services Ltd | Construction | 2020 |
| Giacopazzi's Wholesale Icecream Ltd | Manufacturing | 2018 |
| Gills Trio Ltd | Health & social care | 2024 |
| Golden Spice House Limited | Hospitality | 2026 |
| GR Accountancy and Finance Ltd | Professional & scientific | 2024 |
| Gunsgreen House Trading Limited | Hospitality | 2019 |
| Hemelvaart Homes Ltd | Real estate | 2024 |
| Hippodrome Arts CIC | Arts & recreation | 2012 |
| HMS Financial Limited | Financial services | 2011 |
| Hose World Ltd. | Wholesale & retail | 1995 |
| Infectious Grooves Ltd | Education | 2011 |
| International Tartans CIC | Education | 2025 |
| IRD Design Ltd | Professional & scientific | 2012 |
| J & K Mcneill Limited | Wholesale & retail | 2004 |
| J. & D. Cook Properties Limited | Wholesale & retail | 1996 |
| J.I. Inglis Limited | Construction | 2003 |
| Jadanmill Boatcharters Limited | Transport & logistics | 2015 |
| Jarvis Pickle Holdings Ltd | Financial services | 2024 |
| Jarvis Pickle Limited | Manufacturing | 2016 |
| JOE Russell International Transport Ltd. | Transport & logistics | 2003 |
| Joy's Healthcare Ltd | Health & social care | 2023 |
| JSW Access & Tree Services Limited | Agriculture & fishing | 2019 |
| KBR Consulting Limited | Professional & scientific | 1998 |
| Kirkeiven Ltd | Agriculture & fishing | 2009 |
| KR Custom Works LLP | | 2024 |
| Kult Health & Wellbeing Ltd | Other services | 2025 |
| Kusinton Ltd | Wholesale & retail | 2026 |
| L.J FOX Haulage Ltd | Transport & logistics | 2025 |
| Lannagan Building & Development Ltd | Construction | 2023 |
| LEO Barber Kelso Limited | Other services | 2024 |
| Let's EAT Better CIC | Hospitality | 2025 |
| Lindsay Consulting Limited | Professional & scientific | 2023 |
| Lowland Contract Services Ltd | Administrative & support | 2026 |
| LRJ Farming Limited | Agriculture & fishing | 2023 |
| Lumsdaine Renewables Limited | Energy | 2012 |
| Lunch Concept Ltd | Wholesale & retail | 2020 |
| M Henry Developments Ltd | Construction | 2025 |
| Mainprize Enterprise Ltd | Arts & recreation | 2025 |
| Marine Larder Limited | Agriculture & fishing | 2020 |
| Maverick Ventures (Scotland) Limited | Hospitality | 2021 |
| Meite UK Limited | Wholesale & retail | 2017 |
| Milldown Limited | Hospitality | 2021 |
| Mjfwind Ltd | Professional & scientific | 2026 |
| Moodie Properties Limited | Real estate | 2019 |
| NBT Homes Ltd | Real estate | 2024 |
| NBT Properties Ltd | Real estate | 2025 |
| NBT Resources Ltd | Construction | 2025 |
| Network Online Limited | Information & communication | 2002 |
| New CHU HOI Ltd | Hospitality | 2025 |
| New Strides Theatre | Arts & recreation | 2012 |
| Northern Survey Limited | Construction | 2024 |