| 20/20 Equine Ltd | Wholesale & retail | 2020 |
| 21 Galesbury Road Limited | Households | 2016 |
| 225 Preservation CIC | Transport & logistics | 2025 |
| 3BM Logistics Limited | Transport & logistics | 2025 |
| 4 Claremont Gardens Limited | Real estate | 2016 |
| 55-59 Ipswich Street Management Company Ltd | Extraterritorial | 2008 |
| A Abercrombie Limited | Construction | 2014 |
| A D Brice Livestock Limited | Agriculture & fishing | 2024 |
| A L Engineering & Sons Limited | Wholesale & retail | 2009 |
| A LA Carp Ltd | Manufacturing | 2019 |
| A MAY Holdings Ltd | Health & social care | 2025 |
| A. D. Veale Water Treatment Limited | Water & waste | 2002 |
| A.R. Grinsted & Company Limited | Administrative & support | 1996 |
| AA Foods Chelmsford Limited | Hospitality | 2021 |
| AA Foods Coastal Limited | Hospitality | 2025 |
| AA Foods Essex Limited | Hospitality | 2019 |
| AAD Holdings (East Anglia) Limited | Financial services | 2021 |
| ABC3 GRP Limited | Professional & scientific | 2011 |
| Above & Beyond Kitchens Ltd | Construction | 2023 |
| Absr Consultancy Limited | Administrative & support | 2019 |
| Accelereast Ltd | Professional & scientific | 2025 |
| Access-Able Living Limited | Construction | 2023 |
| Achieving Aspirations Community Interest Company | Health & social care | 2015 |
| Acorn Tree Psychology and Consultancy Services Ltd | Health & social care | 2015 |
| Acorn Tree Training Ltd | Education | 2018 |
| Advanced Aluminium Design Limited | Manufacturing | 2004 |
| Advanced Recovery Systems Ltd | Manufacturing | 2018 |
| Aegg Ltd | Wholesale & retail | 2009 |
| Agri ERP Consulting Ltd | Information & communication | 2022 |
| Agrisynth Ltd | Information & communication | 2021 |
| AHA Courses Limited | Education | 1987 |
| Alcd (Training) Limited | Education | 2001 |
| Alfa (East Anglia) Ltd | Construction | 2025 |
| ALL Secure Self Store Limited | Transport & logistics | 2011 |
| Allied Health Professionals Suffolk CIC | Health & social care | 2011 |
| Allum Electrical Ltd | Construction | 2002 |
| Altr Clinic Ltd | Other services | 2025 |
| Amberwolff Ltd | Arts & recreation | 2024 |
| Amptrust Professional Members Limited | Other services | 2025 |
| Andrew Prentice Partnership Limited | Professional & scientific | 2000 |
| Anglia Consultancy Services Limited | Financial services | 2002 |
| Anglia Freight Limited | Transport & logistics | 1998 |
| Any-Web Limited | Information & communication | 1999 |
| Aovms Limited | Information & communication | 2018 |
| Aqsa Catering Ltd | Hospitality | 2024 |
| Architects Two Ltd | Professional & scientific | 2016 |
| Archmore Limited | Wholesale & retail | 2001 |
| ARK Building Supplies UK Limited | Wholesale & retail | 2023 |
| ARK HR Ltd | Professional & scientific | 2011 |
| ARK Scenery Limited | Arts & recreation | 2024 |
| Aseh Limited | Real estate | 2019 |
| Ashmoat Ltd | Wholesale & retail | 2004 |
| Association of Law Costs Draftsmen Limited(The) | Administrative & support | 1977 |
| Automotive Interim Management Ltd | Professional & scientific | 2003 |
| B & M Seafoods Holdings Limited | Financial services | 2020 |
| B & M Seafoods Limited | Wholesale & retail | 1982 |
| BACK2ROOTS Group Ltd | Wholesale & retail | 2020 |
| Barley Brigg Biogas Ltd | Energy | 2014 |
| Barley Group Ltd | Other services | 2021 |
| Barleyfield Estates Ltd | Real estate | 2025 |
| Barleygroupcloud Ltd | Administrative & support | 2016 |
| Barleygroupnetworks Limited | Information & communication | 2018 |
| Barry Day & Sons Limited | Construction | 2015 |
| Battlesea Hall Farms Ltd | Agriculture & fishing | 2024 |
| BE Well Barn Ltd | Health & social care | 2021 |
| Bedingfield Management Limited | Water & waste | 1988 |
| Beech Land & Developments Limited | Construction | 2017 |
| Belham Limited | Financial services | 2010 |
| BHF Poultry Limited | Agriculture & fishing | 2018 |
| Biashara Holdings Limited | Professional & scientific | 2015 |
| Big Door Limited | Information & communication | 2007 |
| Biologic Group Ltd | Wholesale & retail | 2021 |
| Blackwing Limited | Arts & recreation | 1975 |
| Blaxaudio Limited | Information & communication | 2019 |
| Bloc Management Consultants Limited | Professional & scientific | 1989 |
| Bloomsbury East Ltd | Wholesale & retail | 2025 |
| Blue Sky Exterior Maintenance Ltd | Administrative & support | 2026 |
| BO Mills Associates Ltd | Professional & scientific | 2022 |
| Bookkeeping & Office Administration Services Ltd | Professional & scientific | 2025 |
| Border Farming Limited | Agriculture & fishing | 2018 |
| Bothy Close Management Company Limited | Water & waste | 2019 |
| Bower & Bower Ltd | Transport & logistics | 2023 |
| British & Irish Association of Fastener Distributors Limited | Other services | 2009 |
| Britten Close RTM Company Limited | Households | 2020 |
| Broad Leaf Therapies Ltd | Other services | 2026 |
| Brown & MAY Limited | Extraterritorial | 2022 |
| Brundish Road Residents Limited | Real estate | 2010 |
| Bryan Jordan Construction Limited | Construction | 2021 |
| Buttons Green Farm Limited | Real estate | 2013 |
| C W Logistics Limited | Transport & logistics | 2005 |
| C.E. Davidson Farms Ltd. | Agriculture & fishing | 1997 |
| C.E. Davidson Ltd. | Construction | 1997 |
| C4GROWTH Limited | Professional & scientific | 2011 |
| Cafeye Ltd | Hospitality | 2025 |
| Caitlin ANN Ltd | Professional & scientific | 2024 |
| Camping Gear Hire Ltd | Administrative & support | 2014 |
| Camstar Herbs Limited | Manufacturing | 1979 |
| Camstar Ingredients Limited | Manufacturing | 2011 |
| Carbon Storage Limited | Professional & scientific | 2007 |
| Caresoft Engineering Services Limited | Professional & scientific | 2025 |