| Astra Novellus Ltd | Information & communication | 2020 |
| Axholme Equestrian & Animal Services Ltd | Other services | 2024 |
| C.W.Fields & SON Limited | Manufacturing | 1962 |
| David Caine Cameraman Ltd | Information & communication | 2014 |
| Densifol Cosmetics Ltd | Wholesale & retail | 2025 |
| Fair Hero Canine Fertility Ltd | Other services | 2026 |
| Fair Hero Kennel and Stud Ltd | Agriculture & fishing | 2026 |
| Fire Breathing Unicorn Ltd | Information & communication | 2024 |
| Fixitnowtech Ltd | Administrative & support | 2025 |
| FW Technical Solutions Ltd | Construction | 2011 |
| Grey Horse Ltd | Professional & scientific | 2025 |
| H. & R.E. Lindley Limited | Agriculture & fishing | 1963 |
| HKG Property Holdings Ltd | Financial services | 2021 |
| Investco Msck Ltd | Professional & scientific | 2021 |
| Isle Education Trust Enterprises Limited | Administrative & support | 2016 |
| Lansdowne Garden Centre Limited | Wholesale & retail | 1977 |
| LBC Modular Building Contractors Ltd | Construction | 2017 |
| M.Harm UK Ltd | Other services | 2016 |
| P.Lindley and Sons (Epworth) Limited | Extraterritorial | 1954 |
| Poplar Farm Holdings Ltd | Financial services | 2026 |
| Russell Weston Limited | Administrative & support | 2008 |
| S&S Cargo Ltd | Wholesale & retail | 2019 |
| Salt Yard (Whitstable) Limited | Households | 2020 |
| Sense Workplace Limited | Information & communication | 2023 |
| Speech Therapy Services Ltd | Health & social care | 2011 |
| Sterry & Co Ltd | Real estate | 2017 |
| Steve Bradley Associates Ltd | Professional & scientific | 2014 |
| T. D-R. Investments Limited | Construction | 1986 |
| T.D-R. Group Limited | Financial services | 1985 |
| THE Life & Pensions Network Limited | Financial services | 1998 |
| Victoria Ward Showjumping Coach & Equine Transport Ltd | Agriculture & fishing | 2020 |
| Wentworth Construction Co. Limited | Construction | 1954 |