| DPM (NKT) Developments Ltd | Construction | 2020 |
| DQM Group Holdings Limited | Professional & scientific | 2017 |
| DR. Daniele Torchia Limited | Health & social care | 2014 |
| DRA Farming Limited | Agriculture & fishing | 2019 |
| Drag Bingo Evolution Ltd | Arts & recreation | 2023 |
| Drakeley Stables Limited | Agriculture & fishing | 2017 |
| DRC Holdings Limited | Professional & scientific | 1991 |
| DRC Polymer Products Limited | Manufacturing | 1946 |
| Dream Sales Limited | Wholesale & retail | 2018 |
| DRH Management Services Ltd | Construction | 2024 |
| Drive to Arrive CB Ltd | Education | 2026 |
| Drivecare Ltd | Wholesale & retail | 2007 |
| Drivers Money Ltd | Financial services | 2020 |
| Drivewell Limited | Wholesale & retail | 2025 |
| DRL Business Services Ltd | Administrative & support | 2022 |
| Drmsite Ltd | Information & communication | 2026 |
| DRR Property Ltd | Real estate | 2023 |
| Drug Development Solutions Limited | Professional & scientific | 2022 |
| DS Movements Ltd | Transport & logistics | 2019 |
| DSO Cambridge Limited | Education | 2018 |
| DT Warehousing Limited | Transport & logistics | 2017 |
| Dudley Web Limited | Information & communication | 2026 |
| Dusna Chauhan Associates Ltd | Professional & scientific | 2024 |
| DW Manufacturing Limited | Manufacturing | 2015 |
| DWJ Spitfire Ltd | Professional & scientific | 2023 |
| DWR Consultancy Limited | Information & communication | 2008 |
| Dxnx Ltd | Information & communication | 2008 |
| Dymo Trading Ltd | Wholesale & retail | 2017 |
| Dynamite Coating Ltd | Manufacturing | 2020 |
| E & K Chivers Plumbing & Heating Limited | Construction | 2009 |
| E S Cambridge Limited | Construction | 2013 |
| E S E Groundcare Limited | Other services | 2011 |
| E W Downs Limited | Professional & scientific | 2002 |
| E&S JB Ltd | Health & social care | 2019 |
| E-Salecambridge Limited | Wholesale & retail | 2023 |
| E-Voice Speech Recognition Ltd | Wholesale & retail | 2006 |
| E. & E. Plumridge Limited | Information & communication | 1976 |
| E. Chambers (Mechanical Engineering Services) Limited | Manufacturing | 1975 |
| E.D.M. Projects Limited | Professional & scientific | 2001 |
| E=Qual Limited | Information & communication | 2007 |
| Eagle Engineering Solutions Limited | Education | 2009 |
| Eagle EYE Drone Solutions Ltd | Professional & scientific | 2019 |
| Eagletie Limited | Health & social care | 2009 |
| Eamsa Limited | Professional & scientific | 2015 |
| Earthstone Property Limited | Hospitality | 2026 |
| Easey Property Services Limited | Construction | 2014 |
| East Anglia Farming Contractors Ltd | Financial services | 2021 |
| East Anglia Steel Fabrications Limited | Manufacturing | 2018 |
| East Cambridgeshire Housing Association Limited | Real estate | 2019 |
| East Cambs Street Scene Limited | Other services | 2018 |
| East Cambs Trading Company Limited | Construction | 2016 |
| East Coast Growers Limited | Agriculture & fishing | 2003 |
| East FEN Farms Limited | Agriculture & fishing | 1968 |
| East-Anglia Home Care Ltd | Health & social care | 2020 |
| Eastern Build & Joinery Ltd | Construction | 2019 |
| Eastern Landscape Service Limited | Administrative & support | 2002 |
| Echo Broadband Limited | Information & communication | 2019 |
| Echo Library Properties Ltd | Real estate | 2024 |
| Ecmc Limited | Administrative & support | 2016 |
| Eco Installer and Service Limited | Construction | 2017 |
| Eco Quad Bikes Ltd | Wholesale & retail | 2016 |
| Eco V Investments Limited | Professional & scientific | 2019 |
| Eco V Limited | Professional & scientific | 2018 |
| Eco Verde Energy Limited | Professional & scientific | 2018 |
| Eco Verde Property Limited | Real estate | 2018 |
| Eco Verde Services Limited | Administrative & support | 2017 |
| Ecobin Cleaners Ltd | Administrative & support | 2026 |
| Ecobother Limited | Extraterritorial | 2009 |
| EDG Brands & Solutions Limited | Wholesale & retail | 2021 |
| Edgar Brooks HR Solutions Ltd | Other services | 2001 |
| Edge of Steel Ltd | Construction | 2023 |
| Edify Systems Limited | Information & communication | 2025 |
| Edis of ELY Limited | Wholesale & retail | 2011 |
| Edited Hopefull Limited | Other services | 2022 |
| Edmunds Property Ltd | Real estate | 2023 |
| Edward Matthews Ltd | Wholesale & retail | 2025 |
| EEI Ltd | Real estate | 1998 |
| Eewt Labs Ltd | Information & communication | 2026 |
| EFL Decorating Ltd | Construction | 2025 |
| EH Premises Ltd | Real estate | 2025 |
| EH Software Solutions Ltd | Information & communication | 2018 |
| Eianden Holdings Limited | Financial services | 2022 |
| EJ Lettings Ltd | Real estate | 2012 |
| Ejaar Property Limited | Real estate | 2025 |
| EJM466 Limited | Hospitality | 2025 |
| El&Tay Properties Ltd | Real estate | 2025 |
| Electrical Audit Repair Test & Hire Company Ltd. | Professional & scientific | 1998 |
| Elemental Temple Ltd | Arts & recreation | 2019 |
| Elevation Design and Build Ltd | Construction | 2024 |
| Elidir Productions LLP | | 2024 |
| Elite Farriery and Consultancy Limited | Professional & scientific | 2012 |
| Elite Haylage Limited | Agriculture & fishing | 1999 |
| Elite Labour Cornwall Ltd | Administrative & support | 2023 |
| Elixan Ltd | Real estate | 2020 |
| Ellgia Holdings Limited | Financial services | 2014 |
| Ellgia Limited | Wholesale & retail | 1965 |
| Ellgia Recycling Ltd | Water & waste | 2011 |
| Ellie Senior Ltd | Professional & scientific | 2026 |
| Ellis Heating and Plumbing Ltd | Construction | 2026 |
| Ellis Motorhome Hire Limited | Hospitality | 2024 |