| Jobe Logistics Ltd | Transport & logistics | 2021 |
| Jobworld Consulting Limited | Administrative & support | 2013 |
| Jock's Lodge Community Trust Limited | Arts & recreation | 2025 |
| Joclab LP | | 2015 |
| Jocm Holdings Ltd | Real estate | 2023 |
| Jodie Mann Ltd | Professional & scientific | 2021 |
| Jodihost LP | | 2016 |
| JOE & Co Hairdressing LLP | | 2017 |
| JOE Hall Ltd | Information & communication | 2026 |
| JOE Hill Ltd | Hospitality | 2018 |
| Joe's Bike Garage Ltd | Manufacturing | 2025 |
| Joe's Family Ltd | Hospitality | 2016 |
| Joe's KAF Limited | Hospitality | 2025 |
| Joe's Kitchen Duart Ltd | Hospitality | 2025 |
| Joemartz Ltd | Wholesale & retail | 2024 |
| Joensy Company LP | | 2012 |
| Joge LP | | 2015 |
| Johal Motors Ltd | Transport & logistics | 2024 |
| Johanneses Prods Ltd | Information & communication | 2026 |
| Johenterprises Ltd | Wholesale & retail | 2023 |
| John Abbas Limited | Transport & logistics | 2026 |
| John Bain Edinburgh Ltd. | Wholesale & retail | 2003 |
| John Barr Electronic Services Ltd | Professional & scientific | 2013 |
| John Bowes & Company Limited | Arts & recreation | 2013 |
| John Campbell Construction & Plant Limited | | 1986 |
| John Clephane Limited | | 1972 |
| John Crabbie & Company Limited | Administrative & support | 2006 |
| John Croal (Electrical Contractors) Limited (82/994) | | 1956 |
| John D Young Consulting Limited | Professional & scientific | 2013 |
| John Deere Limited | Wholesale & retail | 1951 |
| John Dick Consulting Limited | Professional & scientific | 2013 |
| John Douglas Farming Limited | Agriculture & fishing | 2019 |
| John Farquharson (GUN & Rifle Maker) Ltd | Manufacturing | 2024 |
| John Finn Financial Management Ltd | Professional & scientific | 2014 |
| John Giblin Management Consulting Limited | Professional & scientific | 2014 |
| John Haig & Company Limited | Professional & scientific | 1925 |
| John Handley Associates Ltd | Professional & scientific | 2011 |
| John Henry Catering Ltd | Hospitality | 2017 |
| John Kay's Shop Ltd | Wholesale & retail | 2017 |
| John Law Media Ltd | Information & communication | 2009 |
| John Macnab Film Ltd | Arts & recreation | 2015 |
| John Marr Mortgage & Protection Ltd | Financial services | 2018 |
| John MC Knight Properties Ltd | Real estate | 2022 |
| John Mcleod Consultancy Limited | Professional & scientific | 2024 |
| John Mcnairney Planning and Management Ltd | Professional & scientific | 2022 |
| John Menzies (Edinburgh) Limited | Professional & scientific | 1906 |
| John Menzies International Limited | Administrative & support | 1985 |
| John Menzies Limited | Professional & scientific | 1960 |
| John Mitchell for Oils Limited | Professional & scientific | 1980 |
| John Morrice Developer Limited | Information & communication | 2025 |
| John P Long Ltd | Water & waste | 2007 |
| John Peter Harrison Consulting Limited | Professional & scientific | 2014 |
| John Rebus Limited | Arts & recreation | 2001 |
| John Renshaw Architect Limited | Professional & scientific | 2019 |
| John Rydale Properties Limited | Real estate | 2024 |
| John Slaven Properties Ltd | Real estate | 2016 |
| John Smith & Company (Edinburgh) Limited | Real estate | 1992 |
| John Smith & SON Group Limited | Information & communication | 1908 |
| John Stalker Productions Limited | Administrative & support | 2012 |
| John Stamp Consulting Limited | Professional & scientific | 2010 |
| John Terrace Surgical Limited | Health & social care | 2019 |
| John Tyre and Sons Ltd | Manufacturing | 2022 |
| Johna Penman Consulting Ltd | Professional & scientific | 2023 |
| Johner Medical UK Limited | Professional & scientific | 2022 |
| Johninglishume.Co Limited | Professional & scientific | 2013 |
| JOHNKAV53 Limited | Professional & scientific | 2016 |
| Johnny Cree & Co. Limited | Administrative & support | 2024 |
| Johnpeniel Communications Ltd | Information & communication | 2025 |
| Johnson & Burt Property Ltd | Real estate | 2023 |
| Johnson & Johnson Medical Limited | Manufacturing | 1991 |
| Johnson and Brothers LP | | 2016 |
| Johnson Distribution Services Ltd | Transport & logistics | 2008 |
| Johnson Family and Property Law Ltd. | Professional & scientific | 2011 |
| Johnson Hamilton and Associates | | 1990 |
| Johnson Legal Nominees Ltd | Professional & scientific | 2007 |
| Johnson Legal Trustees Limited | Administrative & support | 2017 |
| Johnson Property EDI Ltd | Real estate | 2023 |
| Johnson Safety Consulting Ltd | Professional & scientific | 2020 |
| Johnson, Millhouse & Co | | 1993 |
| Johnston & Company Architects Limited | Professional & scientific | 2003 |
| Johnston Financial Asset Management Limited | Financial services | 2020 |
| Johnston Financial Limited | Financial services | 1987 |
| Johnston Oils Limited | Wholesale & retail | 1986 |
| Johnston Property Letting Ltd | Real estate | 2025 |
| Johnston Property Management Ltd | Real estate | 2017 |
| Johnston Smillie Ltd | Professional & scientific | 2004 |
| Johnston-Harris Ltd | Professional & scientific | 1998 |
| Johnstone Equity Limited | Health & social care | 2011 |
| Johnstone Investment Trading (Scotland) Partnership SP | | 2022 |
| Johnstone Management LP | | 2012 |
| Johnstone Stewart Properties Ltd | Real estate | 2023 |
| Johnstounburn Investments Limited | Other services | 2021 |
| Join ME Travel Ltd | Administrative & support | 2023 |
| Joiner Universal LP | | 2012 |
| Joineryfx Ltd | Construction | 2018 |
| Joint Adventures Ltd | Professional & scientific | 2013 |
| Jojava Management Ltd | Extraterritorial | 2012 |
| Joka Property Ltd | Construction | 2021 |
| Jokello Business and Management Consultancy Limited | Administrative & support | 2007 |
| Joker & Thief Ltd | Wholesale & retail | 2021 |