| Boship Lions Farm Hotel Limited | Hospitality | 2015 |
| Bosuns Locker Eastbourne Limited | Wholesale & retail | 2014 |
| Bottle Grove Ltd | Hospitality | 2019 |
| Boujee Hair and Beauty Ltd | Other services | 2020 |
| Bound Bella Limited | Manufacturing | 2025 |
| Bouquet Limited | Wholesale & retail | 2016 |
| Bourne Air Conditioning Limited | Energy | 2022 |
| Bourne Barbers Ltd | Other services | 2025 |
| Bourne Blinds Ltd | Professional & scientific | 2005 |
| Bourne Flooring (East Sussex) Limited | Construction | 2008 |
| Bourne Flooring Holdings Limited | Professional & scientific | 2019 |
| Bourne Scaffolding Limited | Construction | 2022 |
| Bourne to Sail Ltd | Education | 2021 |
| Bourne Track Limited | Construction | 2016 |
| Bournefree Magazine Limited | Manufacturing | 2017 |
| Bourneside Court Limited | Real estate | 2000 |
| Bournestone Limited | Construction | 2013 |
| Boutique DE LA Parfumerie UK Ltd | Wholesale & retail | 2025 |
| Bower Property Rentals Limited | Real estate | 2021 |
| Bowes House (Eastbourne) Limited | Other services | 2004 |
| Bowman Medical Limited | Health & social care | 2022 |
| Box Office Radio Ltd | Information & communication | 2021 |
| Boyce Trenchard Limited | Professional & scientific | 2005 |
| Boyne House (Eastbourne) Management Co. Limited | Households | 1974 |
| BPM Solutions & Services Ltd | Professional & scientific | 2024 |
| BQ Development Ltd | Information & communication | 2018 |
| Bradley K Consulting Services Limited | Information & communication | 2026 |
| Bradleys Bulk Haulage Ltd | Transport & logistics | 2024 |
| Bradsboots Ltd | Wholesale & retail | 2025 |
| Brain Candy Ltd | Information & communication | 2016 |
| Brain's Butchers Limited | Wholesale & retail | 1986 |
| Braintree and Bocking House and Land Company Limited(The) | Construction | 1936 |
| Bramber House RTM Company Limited | Households | 2019 |
| Brampton Commercial Ltd | Real estate | 2003 |
| Branded by JNJ Ltd | Manufacturing | 2024 |
| Brassic Ltd | Financial services | 2022 |
| Braven Wave Enterprise Limited | Construction | 2019 |
| Bravo Barber Ltd | Other services | 2020 |
| Bravo Integral Ltd | Manufacturing | 2022 |
| Braxbess Ltd | Other services | 2023 |
| BRC Dental Ltd | Health & social care | 2018 |
| Bready's Delights Limited | Manufacturing | 2021 |
| Breaking Down Barriers C.I.C. | Education | 2015 |
| Breakthrough Construction Ltd. | Construction | 2018 |
| Breastory Limited | Health & social care | 2020 |
| Breathe With Ease Ltd | Extraterritorial | 2023 |
| Breeze Window Cleaning Ltd | Administrative & support | 2013 |
| Brendling Advanced Aesthetics Limited | Health & social care | 2025 |
| Brenkilo Limited | Construction | 1997 |
| Bresstech Electric Automotive Ltd | Manufacturing | 2019 |
| Brett Enterprise Ltd. | Wholesale & retail | 2024 |
| Brett Tyres Bexhill Ltd | Wholesale & retail | 2024 |
| Bretvents Limited | Manufacturing | 1960 |
| Brewer and Company Limited | Professional & scientific | 2000 |
| Brewster and Sons Ltd | Construction | 2021 |
| Brian Campkin Property Services Ltd | Other services | 2022 |
| Brian Hughes Consulting Ltd | Health & social care | 2021 |
| Brickbond (Sussex) Limited | Construction | 2005 |
| Brickhurst Construction Co. Ltd | Construction | 2007 |
| Bricks Building Limited | Construction | 2016 |
| Bricks Squared Limited | Real estate | 2021 |
| Bridee Whyte Independent Funeral Services Ltd | Other services | 2022 |
| Bridge (DLC) Limited | Real estate | 2008 |
| Bridge Access Scaffolding Ltd | Construction | 2019 |
| Bridge Dental Surgeries Limited | Health & social care | 2011 |
| Bridgepoint Property Limited | Real estate | 2011 |
| Bridgewey Limited | Professional & scientific | 2015 |
| Brightair Products Limited | Professional & scientific | 1993 |
| Brightest Sparks Ltd | Construction | 2023 |
| Brighthold Limited | Real estate | 2025 |
| Brighton Bars Limited | Hospitality | 2016 |
| Brighton Electrical Assemblies Limited | Manufacturing | 1962 |
| Brighton Executive Coaches Ltd | Transport & logistics | 2025 |
| Brighton Happy Limited | Transport & logistics | 2015 |
| Brighton School of Counselling & Psychotherapy Limited | Health & social care | 2021 |
| Brights Sussex Ltd | Hospitality | 2026 |
| Brinefordia Maintenance Limited | Households | 1956 |
| Bristowville Property Co.Limited | Real estate | 1956 |
| Britestyle Limited | Construction | 2008 |
| British Association for Nutrition and Lifestyle Medicine | Other services | 1997 |
| Broad Street Travel Limited | Administrative & support | 2009 |
| Broadwater Dental Clinic Limited | Health & social care | 2021 |
| Broadway Convenience Ltd | Wholesale & retail | 2021 |
| Brocante France Ltd | Wholesale & retail | 2026 |
| Brocks Ghyll Residents Company Limited | Administrative & support | 1994 |
| Broken Stone CC Company Ltd | Real estate | 2021 |
| Bromley Beam Ltd | Health & social care | 2013 |
| Bromley Dental Suite Limited | Health & social care | 2024 |
| Bronwen Jones Ltd | Education | 2021 |
| Brook Tanner Properties Limited | Real estate | 2023 |
| Brooke Dexter Ventures Limited | Financial services | 2026 |
| Brookemead Solutions Limited | Mining & quarrying | 2002 |
| Brookshaw Limited | Administrative & support | 2025 |
| Brookwell Properties Ltd | Real estate | 2021 |
| Brookwell Property Developments Limited | Construction | 2002 |
| Brookwell Property Management Ltd | Real estate | 2000 |
| Brown Brothers Property Development Ltd | Manufacturing | 2020 |
| Bruce Hayter Limited | Professional & scientific | 2023 |
| Brush Strokes (Seaford) Limited | Construction | 2010 |
| Bryant Motors Limited | Wholesale & retail | 2022 |