| 7PLUS7 Maintenance Ltd | Construction | 2016 |
| A C Stoddart & Sons, Colstoun | | 1994 |
| A. & R. Brownlie Limited | Agriculture & fishing | 1996 |
| A.M. Dagg (Plant Hire) Ltd. | Construction | 2003 |
| A.R. Waddell Limited | Agriculture & fishing | 1961 |
| A.W.Gas Ltd | Construction | 2018 |
| Aberlady Mains 1987 Partnership | | 1988 |
| Ac&H 27 Limited | Professional & scientific | 1998 |
| Adaero LLP | | 2017 |
| Aikeyside Partners | | 1998 |
| Alba Glass & Glazing Co Limited | Construction | 2004 |
| Alexander Pollock Limited | Manufacturing | 1969 |
| Altivo Media Limited | Administrative & support | 2025 |
| Andrew Black Limited | Transport & logistics | 2001 |
| Andrew Black Properties Limited | Real estate | 2018 |
| ARE Engineering Limited | Agriculture & fishing | 2007 |
| Athens Ltd. | Transport & logistics | 1999 |
| Awltux Limited | Information & communication | 2004 |
| Ballencrieff Mains Limited Partnership | | 2000 |
| Baron Courts of Prestoungrange & Dolphinstoun Limited | Arts & recreation | 2002 |
| Battle Traders (1745) Limited | Arts & recreation | 2004 |
| Beesknowe Limited | Agriculture & fishing | 1996 |
| Begbie Mains Farming Company | | 1989 |
| Belhaven Brewery Company Limited | Manufacturing | 1944 |
| Belhaven Lobster Limited | Agriculture & fishing | 2021 |
| Bermaline Foods Limited | Manufacturing | 1977 |
| Bermaline Limited | Manufacturing | 1986 |
| Bilston Glen Business Centre Limited | Administrative & support | 2007 |
| BLP 2004-36 Limited | Real estate | 2004 |
| Book-Keeping Services (Scotland) Ltd. | Other services | 2001 |
| Boom Digital Ltd. | Information & communication | 2003 |
| Boys Own Ltd | Agriculture & fishing | 2023 |
| Bradbury Motors Limited | Wholesale & retail | 1968 |
| Bridges Project | Education | 2004 |
| Broomrigg Farm | | 1997 |
| Brown Lindsay + Falgate Farmers | | 1981 |
| Chapman Reid Ltd. | Administrative & support | 2005 |
| Charles River Laboratories Group | Extraterritorial | 1999 |
| Classeye Limited | Construction | 1999 |
| Claymore Lock and Safe Company Limited | Manufacturing | 2001 |
| Clayton Renewables Ltd | Energy | 2014 |
| Clover Country Nursery Limited | Education | 2006 |
| Clyde Link Holdings Limited | Financial services | 2018 |
| Clyde Valley Family Park Limited | Arts & recreation | 2018 |
| Cockenzie Home Farm | | 1986 |
| Courtnet Software Limited | Information & communication | 1998 |
| Credolux Trade L.P. | | 2016 |
| Croft Apartments Limited | Real estate | 2003 |
| Debi Gliori Limited | Arts & recreation | 2000 |
| Delphian Records Ltd. | Information & communication | 2000 |
| Denley Inc Limited | Professional & scientific | 2003 |
| Derek Brown Electrical Services Ltd | Construction | 2005 |
| DLE Engineering Limited | Construction | 2004 |
| Dunbar Pet Food Limited | Wholesale & retail | 2004 |
| Dykegatehead Limited | Agriculture & fishing | 2013 |
| East Coast Organics Ltd | Agriculture & fishing | 2006 |
| East Lothian Ladies Ltd | Professional & scientific | 2018 |
| East Lothian Plumbing & Heating Ltd. | Construction | 2003 |
| East Lothian Produce Limited | Agriculture & fishing | 1998 |
| East Standalane Farming Partnership | | 1999 |
| Easter Ross Coach Company LLP | | 2004 |
| Eastern Exhibition & Display Limited | Manufacturing | 1983 |
| Edin Software Ltd | Information & communication | 2000 |
| Enodatum Limited | Professional & scientific | 2006 |
| Enscot Limited | Construction | 1990 |
| Fenton Barns (Mushrooms) Limited | Extraterritorial | 1987 |
| Fenwick Consulting Limited | Education | 2007 |
| Final Whistle Media Limited | Information & communication | 2002 |
| Finlayson Auto's Ltd | Wholesale & retail | 2006 |
| Fleurtations Plantscapes Ltd | Administrative & support | 1996 |
| Forth Amusements Limited | Administrative & support | 1990 |
| Forth Estuary Engineering (Holdings) Limited | Financial services | 1995 |
| Future and Green Technologies Ltd | Manufacturing | 2007 |
| G Spratt Accountancy Limited | Professional & scientific | 2009 |
| G. Wilson Contracts Limited | Arts & recreation | 1990 |
| George Sharkey & Sons Limited | Construction | 1996 |
| Gibbs Business Solutions Ltd. | Information & communication | 2004 |
| Gilbert Mcclung | | 1988 |
| Gilmerton Gardens | | 2000 |
| Gosford Organics LLP | | 2006 |
| Gowin Services Limited | Water & waste | 2006 |
| Greens of Haddington Ltd. | Other services | 2005 |
| H. & a. Wason (Kamehill) Limited | Agriculture & fishing | 1978 |
| Had-Fab Holdings Limited | Financial services | 2019 |
| Had-Fab Limited | Manufacturing | 1993 |
| Harperdean Landpower Limited | Agriculture & fishing | 1997 |
| Headstart Nursery Schools Limited | Education | 2007 |
| Hedderwick Goal Ltd | Real estate | 2020 |
| Henderson Grass Machinery Limited | Wholesale & retail | 2001 |
| Heywood Tully Limited | Real estate | 1977 |
| Highland Plant Hire Limited | Administrative & support | 1991 |
| HOI Barbers Limited | Other services | 2007 |
| Holland Park Consulting Ltd. | Education | 2013 |
| Hong Xiang Edinburgh Limited | Hospitality | 2017 |
| Howden Renewables Limited | Energy | 2012 |
| Hurley Brown Financial Planning Limited | Financial services | 2002 |
| Illumine Consulting Company Limited | Professional & scientific | 2003 |
| Infinite Apps Limited | Information & communication | 2004 |
| Infracode Consulting Ltd | Information & communication | 2016 |
| J & W Jenkinson | | 2001 |