| RDB Building and Consultancy Services Limited | Professional & scientific | 2018 |
| RDM Electrical (Scotland) Ltd | Construction | 2019 |
| Reality Capture Yorkshire Ltd | Professional & scientific | 2024 |
| Rebeli Electrical Ltd | Construction | 2013 |
| Recovery Investments Corporation LP | | 2021 |
| Redwood Capital Partners III LP | | 2013 |
| Redwood Capital Partners IV LLP | | 2025 |
| Redwood Clinic Limited | Health & social care | 2025 |
| Renovation (Scotland) Limited | Construction | 2017 |
| Renton Heights Limited | Construction | 2026 |
| Renton Homes Limited | Construction | 2024 |
| Retail Design Consultants Limited | Administrative & support | 2007 |
| RHD Transport Limited | Transport & logistics | 2024 |
| Riderecruit Ltd | Administrative & support | 2024 |
| Rikbar Limited | Wholesale & retail | 2004 |
| Ripple Theatre Ltd | Administrative & support | 2025 |
| River Runs Deep Ltd | Professional & scientific | 2024 |
| Riverwood Capital LP | | 2018 |
| RJ Fitness Ltd | Other services | 2025 |
| RJS Parcels Ltd. | Other services | 2019 |
| Rmcg Electrical Limited | Construction | 2023 |
| Rocol (Scotland) Limited | Water & waste | 2007 |
| Ross Brothers (Blenders) Ltd | Professional & scientific | 1959 |
| Rosslyn Property Ltd | Professional & scientific | 2014 |
| Rubix Finance Ltd | Information & communication | 2024 |
| Rydec Fire & Security Solutions Ltd | Public administration | 2023 |
| S D Manufacturing Limited | Manufacturing | 2003 |
| S Maloney Electrical Ltd | Construction | 2017 |
| S2S Community Development C.I.C. | Health & social care | 2026 |
| Safeglass (Europe) Limited | Manufacturing | 1996 |
| Saim Shop & Save Ltd | Wholesale & retail | 2013 |
| Sashseal Windows (Scotland) Limited | Manufacturing | 2009 |
| SB Fitness Scotland Ltd | Other services | 2026 |
| SBD Business Services Ltd | Professional & scientific | 2023 |
| Scottish Leader Limited | Professional & scientific | 1984 |
| Scotwood Holdings Limited | Construction | 2023 |
| Scotwood Interiors Ltd. | Construction | 1986 |
| Scratch Saviours Limited | Extraterritorial | 2023 |
| Sea & Angels Property Limited | Real estate | 2018 |
| Seapines (Blantyre) Limited | Real estate | 2008 |
| Seara International | | 2005 |
| Select Contract Furniture Limited | Manufacturing | 2007 |
| Select Drams Ltd. | Wholesale & retail | 2012 |
| Sfat LP | | 2020 |
| SFC Consulting Limited | Professional & scientific | 2025 |
| Sharadini Ltd | Health & social care | 2014 |
| Simply Install Limited | Extraterritorial | 2010 |
| Simpson-Brown Limited | Real estate | 1961 |
| SJJ Scotland Ltd | Wholesale & retail | 2023 |
| Skin Clinic by Ashleigh Limited | Other services | 2022 |
| Skinworks Limited | Wholesale & retail | 2009 |
| Skytec Aviation Ltd. | Manufacturing | 1993 |
| Slot Chatham Ltd | Construction | 2026 |
| Smith Electrical Limited | Professional & scientific | 2000 |
| SMT (Scotland) Ltd | Construction | 2019 |
| Sofcal Holdings Ltd | Financial services | 2024 |
| Soul Suites Holdings Ltd | Other services | 2025 |
| Specialist Brickwork Services Limited | Construction | 2007 |
| Speedy Plumbing and Heating Ltd | Construction | 2020 |
| Srpm Services Limited | Real estate | 2010 |
| SSL Contracts Limited | Manufacturing | 2023 |
| ST. Andrews Distilling Company Limited | Professional & scientific | 1989 |
| ST. Leger & Co. Limited | Professional & scientific | 2005 |
| Star Home Solutions Ltd | Construction | 2025 |
| Stewart Ventilation Services Limited | Wholesale & retail | 1999 |
| Stirling Fleming Limited | Professional & scientific | 2006 |
| Stonefield Rooms Ltd | Real estate | 2018 |
| Storm Design & Fabricate Ltd. | Manufacturing | 2002 |
| Strachan & Kemp Commercial Services Ltd | Administrative & support | 2014 |
| Strathaven Belting Company Limited | Manufacturing | 1995 |
| Strathmore Leisure Limited | Hospitality | 2018 |
| Strident Publishing Limited | Information & communication | 2005 |
| Studio 4 Films Ltd. | Information & communication | 2003 |
| Summerfield Windows & Doors Ltd | Construction | 2026 |
| Sunrise Delights (Scotland) Ltd | Hospitality | 2025 |
| Supplanter Piping Services Limited | Professional & scientific | 2010 |
| Supreme Tours Limited | Administrative & support | 2004 |
| Susanne Christyne Bridal Design Ltd | Wholesale & retail | 2011 |
| Sutherland Business Solutions Ltd | Professional & scientific | 2011 |
| T.I.M Joiners Ltd | Construction | 2015 |
| T.R. Project Management Ltd | Construction | 2018 |
| T.R. Properties Limited | Administrative & support | 1997 |
| Tailored Home Care Limited | Professional & scientific | 2003 |
| Talas Accounting Ltd | Professional & scientific | 2021 |
| Tara Fabrication Limited | Manufacturing | 1981 |
| Target Healthcare (Wholesale) Limited | Financial services | 2022 |
| Target Healthcare Group Limited | Financial services | 2022 |
| Target Pharmaceuticals Limited | Financial services | 2022 |
| Tartan and Eagle Properties Ltd | Real estate | 2024 |
| Tartan Bathrooms Ltd | Wholesale & retail | 2019 |
| TBS (Scotland) Ltd | Hospitality | 2024 |
| TDC Plumbing Heating & Renewables Ltd | Construction | 2025 |
| Team Services Fire Systems Ltd | Public administration | 2018 |
| Teddies Two Limited | Hospitality | 2011 |
| TG Alba Limited | Construction | 2018 |
| THE Aesthetic Rooms Greenock Ltd | Other services | 2022 |
| THE Beauty Room by HBM Ltd | Other services | 2020 |
| THE Best Curry EK Ltd | Hospitality | 2018 |
| THE Black Prince Scotch Whisky Company Limited | Professional & scientific | 1988 |
| THE Bonspiel Curling Stone Company Limited | Water & waste | 1959 |