| DM Property (EK) Ltd | Real estate | 2024 |
| DMM Transport Ltd. | Transport & logistics | 2007 |
| Dockweiler Scotland Limited | Professional & scientific | 1996 |
| Document Solutions Highlands Ltd | Other services | 2021 |
| Document Solutions North East Ltd | Other services | 2021 |
| Donald Bathroom Solutions Ltd | Construction | 2026 |
| Donnelly's Property Ltd | Real estate | 2023 |
| Dooria (UK) Limited | Manufacturing | 1989 |
| Dopamine Scotland Limited | Real estate | 2024 |
| Dougall Baillie Engineering Limited | Professional & scientific | 2025 |
| DR Labelling Systems Limited | Manufacturing | 1990 |
| Drive Training & Development Ltd. | Professional & scientific | 2006 |
| Drives and Controls Ltd. | Manufacturing | 2003 |
| DS Business Solutions & Services Ltd | Information & communication | 2023 |
| DS Business Solutions Group (Holdings) Ltd | Other services | 2022 |
| DS Communications & IT Solutions Ltd | Information & communication | 2022 |
| DS Finance Ltd | Financial services | 2022 |
| DS HR Business Solutions Ltd | Administrative & support | 2022 |
| DS Property Services & Solutions Ltd | Construction | 2022 |
| DS Sports Management Consultancy Ltd | Professional & scientific | 2022 |
| DSC (EK) Ltd | Other services | 2021 |
| DSH Home Retail Ltd | Real estate | 2024 |
| Dunbar Catering Ltd | Hospitality | 2026 |
| Duncan Inspection Services Ltd | Manufacturing | 2015 |
| Dust Doctor (Scotland) Ltd. | Other services | 2017 |
| Dyna Tune Limited | Wholesale & retail | 2013 |
| Dynamic Concepts (International) Limited | Information & communication | 1998 |
| E-Max Systems Limited | Information & communication | 1998 |
| E.I. Healthcare Ltd | Health & social care | 2026 |
| Eagle Leisure Limited | Wholesale & retail | 2025 |
| Eagleats Limited | Hospitality | 2024 |
| East Kilbride & District Dementia Carers' Group | Health & social care | 2010 |
| East Kilbride & District Engineering Group Training Association Limited | Other services | 1973 |
| East Kilbride Citizens Advice Bureau Limited | Public administration | 2000 |
| East Kilbride Service Centre Ltd | Wholesale & retail | 2014 |
| East Village Cars Ltd | Other services | 2021 |
| Eastkilbride 4 Ltd | Other services | 2006 |
| Easy House Buying Ltd | Real estate | 2024 |
| Eco Heat Grants Ltd | Construction | 2023 |
| Ecosphere Homes Limited | Real estate | 2020 |
| Eighteen by Scannell Property Investments Limited | Real estate | 2023 |
| EK Furniture Outlet Limited | Wholesale & retail | 2014 |
| EK MOT Ltd | Wholesale & retail | 2012 |
| Ekbc Community CIC | Other services | 2025 |
| Ekko Properties Limited | Real estate | 2024 |
| Electric Motor Controls (Scotland) LLP | | 2006 |
| Electric Motor Controls Ltd. | Manufacturing | 2001 |
| Elite Auto Interiors Limited | Wholesale & retail | 2017 |
| Elite Mobile Locksmiths Ltd | Manufacturing | 2011 |
| Elite Roofing Scotland Limited | Construction | 2018 |
| Ellsworth Adhesives Limited | Wholesale & retail | 1990 |
| Empawrium Dog Care Limited | Agriculture & fishing | 2018 |
| EMS Accounting Services Ltd | Professional & scientific | 2023 |
| Enviroclean Contracts Ltd | Water & waste | 2009 |
| EP Ecology Ltd | Professional & scientific | 2020 |
| Ergopart LP | | 2016 |
| Escape to India Ltd. | Administrative & support | 2008 |
| Ethigen Limited | Wholesale & retail | 2000 |
| Ethigen Scotland Limited | Extraterritorial | 2010 |
| Eureka Solutions (Scotland) Limited | Professional & scientific | 1996 |
| Euro Fast Food Distribution Limited | Manufacturing | 2002 |
| Europc Ltd | Wholesale & retail | 2009 |
| Eurowindscreens Limited | Wholesale & retail | 2009 |
| Evalon Carbon Ltd | Professional & scientific | 2025 |
| EVO Enterprises Ltd | Information & communication | 2016 |
| Evoc Electrical Services Limited | Construction | 2025 |
| EW Homes Ltd | Real estate | 2019 |
| Exacta Flooring Services Ltd | Construction | 2019 |
| Extreme Transport Services Ltd | Transport & logistics | 2017 |
| F Healey Ltd | Other services | 2021 |
| Farm Gates (UK) Limited | Wholesale & retail | 2011 |
| Fergie Sent ME Ltd | Extraterritorial | 2012 |
| Fergus Fit Ltd | Other services | 2014 |
| Ferrie Healthcare Limited | Health & social care | 2021 |
| Feyer Investments LP | | 2021 |
| Fire Control (Glasgow) Limited | Water & waste | 1984 |
| Fire Risk Solutions Network Limited | Education | 2012 |
| Fire Sprinkler Controls Ltd | Public administration | 2024 |
| Firesafe Academy Ltd | Information & communication | 2025 |
| Firstflow Technical Services Ltd | Manufacturing | 2005 |
| Fisnar Europe Ltd | Manufacturing | 2010 |
| Fitness Chicks Community Ltd | Other services | 2023 |
| Fitness Chicks Strength Ltd | Other services | 2025 |
| Fitzpatrick Roofing Ltd | Construction | 2017 |
| Fleming Masonry Ltd | Construction | 2021 |
| FLY Caledonia Airways Ltd | Transport & logistics | 2021 |
| FM & AF Limited | Real estate | 2025 |
| Fmkm Limited | Extraterritorial | 2009 |
| FMR Electrical Ltd | Construction | 2019 |
| Fraz Holdings LP | | 2016 |
| Frigga Associates LP | | 2017 |
| Frigo Installations Limited | Other services | 2024 |
| FTD Limited | Manufacturing | 1994 |
| Funktional Fitness Training Ltd | Arts & recreation | 2019 |
| Fuse-Fit Limited | Other services | 2016 |
| G C Auto Sales Limited | Wholesale & retail | 2022 |
| G&B Catering Limited | Hospitality | 2016 |
| GCH Precision Engineering Ltd. | Manufacturing | 2006 |
| GDK International Limited | Hospitality | 2016 |
| Geniitec Ltd | Professional & scientific | 2020 |