| 47B Holdings Limited | Financial services | 2025 |
| 9.75 Scotland Ltd | Hospitality | 2025 |
| A Clark Building Solutions Ltd | Construction | 2022 |
| A K P Scotland Limited | Construction | 1990 |
| A One Jointing Systems Limited | Energy | 2005 |
| A1 Mobile Tyre Fitting Ltd | Wholesale & retail | 2025 |
| A2Z Storall Ltd | Arts & recreation | 2026 |
| Aari Consultancy Limited | Professional & scientific | 2020 |
| Aber GAS Services Limited | Wholesale & retail | 2002 |
| Aberdrinks Limited | Extraterritorial | 2012 |
| AC WPC Ltd | Professional & scientific | 2026 |
| AC6 Consultancy Ltd | Professional & scientific | 2025 |
| Advanced Mailing Solutions Limited | Administrative & support | 2002 |
| AE Waste Management Ltd | Water & waste | 2024 |
| Aeig Scotland Limited | Professional & scientific | 2024 |
| Aeron Automation Limited | Manufacturing | 1984 |
| Agile Identity Ltd | Information & communication | 2019 |
| Ailsa Lift Truck Rentals Ltd. | Real estate | 2009 |
| Ailsa Machinery Limited | Manufacturing | 1993 |
| Aimo Ltd | Construction | 2025 |
| Airglove Medical Limited | Manufacturing | 2022 |
| Ajaw Limited | Hospitality | 2026 |
| AKP Group Limited | Professional & scientific | 2008 |
| Albacap Limited | Financial services | 2018 |
| Albaco Limited | Financial services | 2018 |
| Albyn Bond (Airdrie) Limited | Professional & scientific | 1977 |
| Aldomann (Clydebank) Ltd | Extraterritorial | 2016 |
| Aldomann (Dumbarton) Ltd | Wholesale & retail | 2016 |
| Aldomann (Partick) Limited | Wholesale & retail | 2016 |
| Alex Mcdougall (Mowers) Limited | Manufacturing | 1996 |
| Alexanders Wealth Management Ltd | Financial services | 2017 |
| Alliance Sanitary Products Limited | Wholesale & retail | 2004 |
| Allied Retail Sourcing Ltd | Administrative & support | 2017 |
| Alloa Grove Homes Limited | Construction | 2025 |
| Alpha Machine Technologies Limited | Professional & scientific | 1996 |
| Alyntra Group Ltd | Construction | 2025 |
| AMG Property Partners Ltd | Construction | 2025 |
| AMH Plumbing & Heating Ltd | Construction | 2023 |
| Amir & Co Legal Ltd | Administrative & support | 2025 |
| Andromeda Investment Corporation LP | | 2021 |
| Anglian Home Improvements Green Power Limited | Professional & scientific | 1991 |
| Anglian Home Improvements Solar Energy Limited | Professional & scientific | 1993 |
| Anglian Home Improvements Solar Thermal Limited | Professional & scientific | 1990 |
| Anglian Solar Energy Limited | Professional & scientific | 1993 |
| Annapurnaa Foods Limited | Hospitality | 2026 |
| Anzada&Co Ltd | Wholesale & retail | 2019 |
| APC Holdings Glasgow Ltd | Construction | 2022 |
| API Technology Ltd | Information & communication | 2018 |
| Apple Tree Pictures Ltd | Information & communication | 2024 |
| Arbortech Scotland Ltd | Administrative & support | 2022 |
| Argyle Diesel Electrics Limited | Wholesale & retail | 1963 |
| Artisan Buon Giorno Bakery East Kilbride Ltd | Wholesale & retail | 2016 |
| Artisan Buon Giorno Bakery Greenhills Ltd. | Wholesale & retail | 2014 |
| Artisan Buon Giorno Bakery Limited | Manufacturing | 2013 |
| Artisan the Bakery Ltd | Manufacturing | 2017 |
| Asad Cuts Ltd | Other services | 2024 |
| Assante Ltd | Transport & logistics | 2023 |
| At&T Enterprises Ltd | Transport & logistics | 2025 |
| Atexis Solutions Ltd | Professional & scientific | 2024 |
| Atifparts Ltd | Other services | 2019 |
| Audrieu LP | | 2009 |
| Auto B Holdings Ltd | Wholesale & retail | 2022 |
| Automatic Control Systems (Scotland) Ltd. | Manufacturing | 1997 |
| Avondale Partners Ltd | Arts & recreation | 2011 |
| AYR SA 2 Limited | Other services | 2024 |
| AYR SA Ltd | Real estate | 2024 |
| Azimuth Healthcare Solutions Ltd | Professional & scientific | 2013 |
| Azure Fire Protection Ltd | Construction | 2022 |
| BA Technical Solutions Ltd | Information & communication | 2025 |
| Back Office Payroll Processing Limited | Administrative & support | 2025 |
| Bailey & Tibbs Gifts and Decor LLP | | 2026 |
| Bailey & Tibbs Interiors Ltd | Wholesale & retail | 2020 |
| Balkan Proof LIC Ltd | Extraterritorial | 2010 |
| Balkan Proof Limited | Hospitality | 2010 |
| Barclay Control Systems Limited | Manufacturing | 2021 |
| Bartle Electrical Ltd | Construction | 2022 |
| Barzini Official Limited | Wholesale & retail | 2025 |
| BB Factory Shops Ltd | Wholesale & retail | 2010 |
| Bellini Nails Ltd | Other services | 2018 |
| Big Dawg Media Limited | Manufacturing | 2022 |
| Big S Design Scotland Ltd | Information & communication | 2020 |
| Blantyre Electrical Ltd | Manufacturing | 2022 |
| Blantyre Park Building Services Limited | Construction | 2016 |
| Blantyre Park Facility Services Ltd | Construction | 2015 |
| Blantyre Park Properties Ltd. | Real estate | 2002 |
| Blantyre Park Services Ltd | Construction | 2006 |
| Blau Investment Partners LP | | 2015 |
| BLUE789 Limited | Wholesale & retail | 2014 |
| BM Fryers Ltd | Hospitality | 2024 |
| Bonnie Brae Group Ltd | Real estate | 2025 |
| BOOTHS4YOU Limited | Arts & recreation | 2015 |
| Border Collie LP | | 2018 |
| Borland & Co Limited | Professional & scientific | 2011 |
| Bovill & Boyd (Engineering) Limited | Wholesale & retail | 1986 |
| Boxshop Limited, (the) | Manufacturing | 1986 |
| Breamen Capital LP | | 2015 |
| Bresr Limited | Wholesale & retail | 2026 |
| Bruce Events Ltd | Extraterritorial | 2019 |
| Buchanan Burton Limited | Professional & scientific | 2012 |
| Burn, Mckenzie & Co. Limited | Professional & scientific | 1951 |