| 333 Edin Ltd. | Professional & scientific | 2015 |
| Achievaball Limited | Arts & recreation | 2022 |
| Adept Window Cleaning Ltd | Other services | 2007 |
| Alan Miller Construction Consultants Ltd | Construction | 2019 |
| Alchebeo Ltd | Manufacturing | 2025 |
| AT Birkhill House CIC | Other services | 2021 |
| AT Travel Limited | Transport & logistics | 2020 |
| Aurora Cleaning Edinburgh Ltd | Other services | 2012 |
| Axis Joinery Ltd | Construction | 2023 |
| BBS (Scotland) Ltd | Wholesale & retail | 2020 |
| Birkenside Wind Turbine Ltd | Energy | 2012 |
| Body Repair Services (Earlston) Limited | Wholesale & retail | 1972 |
| Borders Animal Welfare Association | Administrative & support | 2008 |
| Borders Roof and Render Clean Ltd. | Administrative & support | 2018 |
| Briganti Ocean Systems Ltd | Manufacturing | 2025 |
| Bruce Colvine Electrical Limited | Construction | 2019 |
| Chambers Legal Ltd | Professional & scientific | 2012 |
| Coffee END Station Ltd | Hospitality | 2025 |
| Coorie Away Limited | Hospitality | 2022 |
| Cowdenknowes Mains Farm Limited | Agriculture & fishing | 1946 |
| Cross Section Industries Ltd | Information & communication | 2021 |
| Crossfit JXL Ltd | Arts & recreation | 2015 |
| CWT Sporting Ltd | Agriculture & fishing | 2026 |
| Danny Simpson Plumbing & Heating Limited | Construction | 2023 |
| Davidson Land Services Ltd | Construction | 2015 |
| DCP Renewables Ltd | Wholesale & retail | 2025 |
| Derek J Anderson Quantity Surveying Services Ltd | Professional & scientific | 2015 |
| Douglas Scott Plumbing & Slating Limited | Construction | 2011 |
| Ettrick Corporate Limited | Professional & scientific | 2025 |
| Ewan Mcgeorge Driver Hire Limited | Administrative & support | 2017 |
| Falcon's Gym Ltd | Arts & recreation | 2025 |
| Fear a Bhata Limited | Transport & logistics | 2014 |
| Forbes Electrical Ltd | Construction | 2023 |
| Fraser Lothian Joiners Limited | Construction | 2019 |
| Game of Bones Ltd | Administrative & support | 2017 |
| Greenknowe Property Developments Limited | Real estate | 2023 |
| Guardian Auto Repair Ltd | Wholesale & retail | 2025 |
| Hamish Morison Farming Limited | Agriculture & fishing | 1989 |
| Hamish Morison Poultry Limited | Agriculture & fishing | 2012 |
| Hexotherm Ltd | Manufacturing | 2022 |
| Iron and Ember Ltd | Administrative & support | 2026 |
| Jeco Consulting Ltd | Professional & scientific | 2022 |
| JWG Miller and Company Limited | Construction | 2007 |
| JWG Miller Property Ltd | Real estate | 2007 |
| JXL Fitness Ltd | Arts & recreation | 2020 |
| Kidgate Consulting Ltd | Professional & scientific | 2026 |
| L.M.R. Training Services Ltd. | Extraterritorial | 1998 |
| Leader Water Services Ltd | Construction | 2025 |
| Legerwood Investments Limited | Agriculture & fishing | 2013 |
| Lothian & Borders Training Services Ltd | Wholesale & retail | 2001 |
| Manning Medical Ltd | Health & social care | 2011 |
| Mcgarva Fitness Limited | Wholesale & retail | 2023 |
| Mcginlay GAS Ltd | Construction | 2021 |
| Mclean Hospitality Ltd | Hospitality | 2024 |
| Michael Scott Joiners Limited | Construction | 2002 |
| Ordinis Group Ltd | Real estate | 2026 |
| Paul's Vehicle Garage (PVG) Ltd | Wholesale & retail | 2024 |
| Pixelbase Studio Limited | Information & communication | 2018 |
| POW Heating Ltd | Construction | 2019 |
| Priya Fine Indian Cuisine Ltd | Manufacturing | 2019 |
| Redpath Livestock Ltd | Transport & logistics | 2009 |
| Rhymers Transport and Recovery Limited | Wholesale & retail | 2020 |
| Robert Henderson Limited | Construction | 2016 |
| Rodger Farms Ltd | Agriculture & fishing | 2018 |
| RPA Builders Limited | Construction | 2019 |
| Rukker Limited | Real estate | 2014 |
| Scotts Garage Earlston Limited | Wholesale & retail | 2016 |
| Sharp Talon Limited | Extraterritorial | 2020 |
| Skills (Scotland) Testing Services Limited | Education | 2011 |
| Teatreasuresuk Limited | Wholesale & retail | 2021 |
| Terra Mechanica Maris Ltd | Manufacturing | 2023 |
| THE Cleangineers Limited | Administrative & support | 2022 |
| THE Other END Ltd | Hospitality | 2026 |
| Tony Hibberd Limited | Administrative & support | 2025 |
| Top of the Mops Cleaning Limited | Administrative & support | 2009 |
| W Donaldson & SON Ltd. | Wholesale & retail | 2012 |
| Young AI Ltd | Information & communication | 2023 |