| A Winterbotham Ltd | Professional & scientific | 2015 |
| Aceo Europe Ltd | Wholesale & retail | 2025 |
| Cadster House Consulting Ltd | Professional & scientific | 2021 |
| Charles Martell and SON Limited | Manufacturing | 2006 |
| Chuva Solution Limited | Wholesale & retail | 2018 |
| Ciriaco Limited | Administrative & support | 2019 |
| Clare for YOU Ltd | Professional & scientific | 2026 |
| Cruse Limited | Health & social care | 2024 |
| Dave Williams Glazing and Property Care Ltd | Construction | 2020 |
| Deadloud Limited | Manufacturing | 2020 |
| Downing Educational Associates Ltd | Education | 2010 |
| E.C. Investments (Gloucestershire) Limited | Health & social care | 2002 |
| Ekhaya Building Consultants (EBC) Limited | Construction | 2024 |
| Encert Limited | Professional & scientific | 2007 |
| Ferguson Organic Ltd | Agriculture & fishing | 2023 |
| Green Pensions Ltd | Financial services | 2026 |
| Greg Tovey Construction Limited | Construction | 2000 |
| Holford Malone Developments Ltd | Construction | 2015 |
| I.O.Shen Knives UK Limited | Wholesale & retail | 2017 |
| Icon Two Limited | Real estate | 2003 |
| JSH Quality Services Ltd | Professional & scientific | 2024 |
| Kempley COW Company Limited | Agriculture & fishing | 2012 |
| Lansker Homes Limited | Real estate | 2020 |
| Learning Joy Success Ltd | Education | 2009 |
| Linton Festival CIC | Arts & recreation | 2011 |
| Livestock International Limited | Agriculture & fishing | 2007 |
| Lukesfilm Ltd | Information & communication | 2021 |
| M & M Lock Secure Limited | Construction | 2003 |
| Mammoth Two Ltd | Real estate | 2016 |
| Maxtrack Limited | Wholesale & retail | 2004 |
| MK Project Services Ltd | Construction | 2020 |
| My Green Pension Investments Ltd | Financial services | 2025 |
| Newent Plant Hire Ltd | Extraterritorial | 2020 |
| NTC & Co. Ltd | Manufacturing | 2021 |
| Pretty Ethical Ltd | Wholesale & retail | 2025 |
| Quintain Investments Ltd | Financial services | 2023 |
| Secoran Ltd | Information & communication | 2018 |
| Shorter Timlin Archivists Limited | Arts & recreation | 2012 |
| Simon Pratley Limited | Professional & scientific | 2006 |
| Stallard Farms Limited | Agriculture & fishing | 1960 |
| Standing Stones Whisky Ltd | Wholesale & retail | 2021 |
| Sun Coast Properties Limited | Real estate | 2004 |
| TDE Limited | Professional & scientific | 2011 |
| THE Cotswold Proposal Planners Ltd | Other services | 2022 |
| THE Leglock Company Ltd | Wholesale & retail | 2023 |
| THE Mammoth Pack Ltd | Arts & recreation | 2022 |
| THE Patchwork Shop Limited | Wholesale & retail | 2017 |
| Thornhouse Cottage Limited | Extraterritorial | 1996 |
| Three Shires Windows Limited | Extraterritorial | 2020 |
| Tovey Farming Limited | Agriculture & fishing | 2008 |
| Tweedale Education Partners Ltd | Education | 2018 |
| Two Rivers Homes Ltd | Construction | 2022 |
| Whittocks END Accountancy Ltd | Professional & scientific | 2010 |
| Your Pension Your Planet CIC | Education | 2025 |