| S E Hughes Limited | Construction | 2010 |
| S. J. Ecroyd Ltd | Financial services | 2023 |
| Sage Residential Ltd | Real estate | 2020 |
| Sage Residential Surveying Ltd | Professional & scientific | 2025 |
| Salt Folk Ltd | Administrative & support | 2026 |
| SAM DIX Carpentry Ltd | Construction | 2022 |
| Samboards Limited | Health & social care | 2019 |
| Sandfields Farm LLP | | 2007 |
| Sandwich Station (Dursley) Limited | Wholesale & retail | 2025 |
| Sango Consulting Ltd | Administrative & support | 2006 |
| Sanigar Battery Storage 1 Limited | Construction | 2021 |
| Sarah Albiston Limited | Information & communication | 2011 |
| Saves Nine Ltd | Manufacturing | 2025 |
| Scarletts Management Services Limited | Professional & scientific | 2019 |
| Scars to Strength Ltd | Wholesale & retail | 2025 |
| Scorpion Precision Tooling UK Limited | Manufacturing | 2016 |
| Scott Garage Services Limited | Wholesale & retail | 2024 |
| Scott Robinson Ltd | Construction | 2014 |
| Seddon Technical Consulting Limited | Professional & scientific | 2023 |
| Segger Microcontroller UK Limited | Information & communication | 2022 |
| Seraphine Software Limited | Information & communication | 2025 |
| Serif Limited | Professional & scientific | 2003 |
| Severn Dance Studios Limited | Education | 2025 |
| Severn Electrical Services Ltd | Construction | 2021 |
| Severn Solar Limited | Construction | 2023 |
| SGB Property Developments Ltd | Construction | 2022 |
| Shades of Comfort Limited | Manufacturing | 2005 |
| Sharp Heating & Electrical Ltd | Construction | 2020 |
| SHE Rises Ltd | Manufacturing | 2025 |
| Shear Delight (Gloucestershire) Limited | Other services | 2014 |
| Sheena’S Family Inns Limited | Hospitality | 2024 |
| Sherborne Coaching Limited | Education | 2026 |
| Shire Window Solutions Ltd | Construction | 2015 |
| Shivani Consulting Limited | Information & communication | 2007 |
| Silver Birches Limited | Professional & scientific | 2018 |
| Silver Street Micro PUB Limited | Hospitality | 2022 |
| Simply Sweepers Limited | Administrative & support | 2017 |
| Simreed Limited | Professional & scientific | 1977 |
| SIX4 Properties Limited | Real estate | 2019 |
| SK (Dursley) Limited | Hospitality | 2025 |
| SKI Nordic Style UK Ltd | Wholesale & retail | 2024 |
| Sky Phones Ltd | Information & communication | 2025 |
| Slevinth Heaven Ltd | Information & communication | 2019 |
| SLS Accountants Ltd | Professional & scientific | 2005 |
| Small Cell Forum Limited | Other services | 2007 |
| Smart Sprite Limited | Professional & scientific | 2012 |
| Smooth Lets Limited | Real estate | 2019 |
| Solent Stevedores Container Terminal Limited | Transport & logistics | 2022 |
| Solent Stevedores Limited | Transport & logistics | 2000 |
| Solent Terminal Management Limited | Transport & logistics | 2015 |
| Sound + Motion Limited | Information & communication | 2018 |
| South Western Solutions Ltd | Construction | 2008 |
| Southwest Discs Ltd | Wholesale & retail | 2026 |
| Spice Fusions Dursley Limited | Hospitality | 2019 |
| SPK Trading Limited | Transport & logistics | 2022 |
| Sportspoints Plus Limited | Wholesale & retail | 1997 |
| Square One Design Supply Limited | Manufacturing | 1996 |
| Squidy Tech Ltd | Information & communication | 2025 |
| ST George's Flats Ltd | Real estate | 2025 |
| ST Marks Rise Dursley Management Limited | Households | 2020 |
| Stamp Architecture and Design Ltd | Professional & scientific | 2025 |
| Standle Limited | Information & communication | 2016 |
| Stanfred Limited | Administrative & support | 2024 |
| Stangard Design Solutions Limited | Construction | 2003 |
| Stangard Holdings Limited | Professional & scientific | 2024 |
| Star Anise Arts Cafe Ltd | Hospitality | 2007 |
| Stegosaurus Enterprises Limited | Construction | 2019 |
| Straight BAT Communications Ltd | Professional & scientific | 2012 |
| Stroud Care Property Limited | Health & social care | 2014 |
| Structure 1 Timberworks Ltd | Construction | 2024 |
| Sun Life Flats Limited | Real estate | 2021 |
| Superbike Surgery Limited | Manufacturing | 2001 |
| Suregate Limited | Manufacturing | 2015 |
| Surprise Aviation Limited | Professional & scientific | 1993 |
| T&C Plastering Limited | Construction | 2024 |
| T-Rex Investments Limited | Real estate | 2019 |
| T.A. Willcox Building Contractors Ltd. | Construction | 2008 |
| Tarr Electrical Ltd | Construction | 2010 |
| Taylored Construction Services Ltd | Construction | 2023 |
| TB Valeting & Detailing Limited | Administrative & support | 2024 |
| TCJ Waste Solutions Ltd | Water & waste | 2017 |
| Tengu Services Ltd | Administrative & support | 2025 |
| That Mortgage Woman Ltd | Financial services | 2023 |
| THB Global Consulting Ltd | Information & communication | 2025 |
| THE Beauty Room CAM Ltd | Other services | 2024 |
| THE Car Clinic (Kingswood) Ltd | Wholesale & retail | 2018 |
| THE Cast Studio London Ltd | Professional & scientific | 2025 |
| THE Chantry Centre | Arts & recreation | 2009 |
| THE Chantry Centre Trading Company Ltd | Arts & recreation | 2010 |
| THE Cork & Collar Ltd | Wholesale & retail | 2025 |
| THE Cotswold Way Farm Limited | Agriculture & fishing | 2014 |
| THE Doorstep Sandwich Shop Limited | Hospitality | 2024 |
| THE Drain MAN South West Ltd | Administrative & support | 2024 |
| THE Dursley Cellar Ltd | Wholesale & retail | 2026 |
| THE Early Years Village CIC | Education | 2024 |
| THE Energy Bank Limited | Financial services | 2022 |
| THE Engine Hub CIC | Professional & scientific | 2021 |
| THE Friends of Julius and Dora Childrens' Centre Maseno | Other services | 2007 |
| THE Glass Foundry Ltd | Arts & recreation | 2019 |
| THE Hummingbird Cafe Bar & Restaurant Limited | Hospitality | 2012 |