| Attivo Business Group Ltd | Wholesale & retail | 2019 |
| Atvantage Limited | Agriculture & fishing | 2012 |
| AU Pyramid Ltd | Real estate | 2026 |
| Audrey Parker Limited | Administrative & support | 2011 |
| Auld Classic Bar Limited | Hospitality | 2009 |
| Aura Aesthetics Fife Limited | Other services | 2025 |
| Auslec Ltd | Construction | 2023 |
| AUTHENTIK9 Ltd | Professional & scientific | 2025 |
| Autodiagnostik Ltd. | Wholesale & retail | 2010 |
| Autotech Fife Ltd | Wholesale & retail | 2020 |
| AV Department Limited | Information & communication | 2005 |
| Avazen Ltd | Other services | 2023 |
| AVBCTRADING2017 Ltd | Arts & recreation | 2017 |
| Avenue Care Services Limited | Health & social care | 2014 |
| Avenue Scotland Limited | Administrative & support | 1993 |
| Avfuse Limited | Administrative & support | 2012 |
| Avoluxe Ltd | Wholesale & retail | 2025 |
| Avtek (Scotland) Ltd | Construction | 2014 |
| Avtek Group Limited | Financial services | 2024 |
| AWE Property Holding Ltd | Real estate | 2018 |
| AWG Consultants Limited | Information & communication | 2002 |
| Axis People Limited | Administrative & support | 2025 |
| Axon Cable Limited | Manufacturing | 1990 |
| AY Burgers Ltd | Hospitality | 2025 |
| AYE Group Ltd | Administrative & support | 2009 |
| Ayrshire Carbon Reclamation Limited | Manufacturing | 2024 |
| AYT Foods Ltd | Wholesale & retail | 2023 |
| Azmi Aydin Limited | Other services | 2026 |
| B & F Decorating Services Ltd | Construction | 2013 |
| B and J Property Limited | Financial services | 2019 |
| B M ZEE Ltd | Transport & logistics | 2024 |
| B.E.C Access Ltd | Construction | 2025 |
| Babcock Design & Technology Limited | Professional & scientific | 1997 |
| Babcock Marine (Clyde) Limited | Professional & scientific | 2001 |
| Babcock Marine (Rosyth) Limited | Manufacturing | 2007 |
| Baby Ballerz Limited | Arts & recreation | 2015 |
| Back 2 Earth Electrical (Fife) Ltd | Construction | 2020 |
| Backstage Group Ltd | Other services | 2017 |
| Bagpipe Tours Ltd | Administrative & support | 2018 |
| Bailiwick Communications Limited | Professional & scientific | 2023 |
| Baird Taxis Ltd | Transport & logistics | 2005 |
| Balbs International Ltd | Real estate | 2024 |
| Balmule Huts Ltd | Agriculture & fishing | 1999 |
| Baltic Transport Ltd | Transport & logistics | 2025 |
| Banach Property Ltd | Real estate | 2021 |
| Bandctraining Ltd | Education | 2026 |
| Bankhead Property Co. Ltd | Real estate | 1993 |
| Bar Solar Limited | Hospitality | 2019 |
| Barberek Ltd | Other services | 2026 |
| Barden Development Limited | Real estate | 2018 |
| Barden Hogg Limited | Real estate | 2020 |
| Barology Harrogate Limited | Hospitality | 2025 |
| Barry Morrison Property Holdings Limited | Real estate | 2025 |
| Barry Morrison Rendering Ltd | Construction | 2021 |
| Barryhanlon Ltd | Construction | 2026 |
| Bars & Bistros Tronc Ltd | Professional & scientific | 2024 |
| Bars and Rooms at NO 18 Ltd | Extraterritorial | 2020 |
| Basecommit Limited | Information & communication | 2026 |
| Baseline Management Services Limited | Professional & scientific | 2014 |
| Bathroom Centre Fife Ltd | Construction | 2019 |
| Bathroom Design and Installation Limited | Construction | 2019 |
| Baxter Baseline Concepts Ltd | Professional & scientific | 2025 |
| Bay Hardware Ltd | Wholesale & retail | 2024 |
| Bay Kutz Limited | Other services | 2007 |
| Bay of Bengal Restaurant Ltd | Hospitality | 2020 |
| Bayleaf TWB Ltd | Real estate | 2014 |
| Bayne Stevenson Associates Ltd. | Professional & scientific | 2006 |
| Bayu Resources Ltd | Professional & scientific | 2018 |
| Bb&B Spice Ltd | Hospitality | 2026 |
| BC Enviro Services Limited | Water & waste | 2003 |
| BCO Golden Properties Ltd | Real estate | 2021 |
| BE Active Fife C.I.C. | Wholesale & retail | 2017 |
| BEA Lighting Ltd | Construction | 2018 |
| Bear & Boomer Investments Ltd | Real estate | 2021 |
| Bearfox Joiners Limited | Construction | 2026 |
| Beautique Aesthetics Skin Clinic Limited | Health & social care | 2020 |
| Beer for Good CIC | Hospitality | 2016 |
| Being Frank Limited | Extraterritorial | 2015 |
| Beinn Ltd | Professional & scientific | 2019 |
| Bela’S Cakes Ltd | Hospitality | 2023 |
| Bella Rosyth Ltd | Hospitality | 2020 |
| Belmont Bars Ltd | Hospitality | 2016 |
| Beltanimo Properties Limited | Real estate | 2018 |
| Benji Physio Limited | Other services | 2019 |
| Benthead Developments Limited | Real estate | 1990 |
| BER Utilities Ltd | Construction | 2022 |
| Berrylaw Farms Limited | Extraterritorial | 2010 |
| Berturelli Cornice Restoration & Repairs Ltd | Construction | 2026 |
| Beryllium Property Limited | Real estate | 2020 |
| Bespoke Golf Tours Limited | Administrative & support | 2015 |
| Bespoke Hair and Beauty Limited | Other services | 2014 |
| Best Hand Car Wash Dunfermline Limited | Administrative & support | 2019 |
| Best Mortgage Direct Limited | Financial services | 2008 |
| Best Way Living Ltd | Wholesale & retail | 2025 |
| Better Humans Ltd | Health & social care | 2015 |
| Better Physio Ltd | Health & social care | 2016 |
| Beyonder Ltd | Education | 2013 |
| BG Autocare Ltd | Wholesale & retail | 2025 |
| BG Services Transport Limited | Transport & logistics | 2020 |
| Bianco Bathrooms and Interiors Limited | Construction | 2025 |