| Softek IT Solutions(Uk) Ltd | Information & communication | 2019 |
| Sogni D'Oro Property Company Limited | Administrative & support | 2004 |
| SOI WAD Ltd | Hospitality | 2025 |
| Soits Tech Ltd | Information & communication | 2024 |
| Sola Scot Ltd | Education | 2023 |
| Solar Engineer Ltd | Professional & scientific | 2025 |
| Solargy Ltd | Construction | 2022 |
| Solas Renewable Energy Holdings Limited | Financial services | 2018 |
| Soldybra Ltd | Wholesale & retail | 2025 |
| Solstice Consultancy Limited | Professional & scientific | 2020 |
| Soma Energy Ltd | Manufacturing | 2024 |
| Sonis Smart Security Limited | Professional & scientific | 2004 |
| Sound Wealth Planning Limited | Financial services | 2024 |
| Sourcing Box Limited | Professional & scientific | 2013 |
| Souter Money Management Limited | Financial services | 2021 |
| Souter Property Management Ltd | Real estate | 2023 |
| Southern Spirit Ltd | Wholesale & retail | 2009 |
| Spac Ltd | Real estate | 2026 |
| Specialist Suppression Solutions Limited | Professional & scientific | 2017 |
| Specialist Vehicle Transportation Ltd | Transport & logistics | 2023 |
| Specific IT Solutions Limited | Financial services | 2012 |
| Spectrum Systems Design Limited | Information & communication | 2026 |
| Speedy Carz Limited | Transport & logistics | 2024 |
| Spex Direct (Scotland) Limited | Wholesale & retail | 2006 |
| Spex in the City (Dunfermline) Limited | Administrative & support | 2007 |
| Spice Lounge - Kinross Ltd | Hospitality | 2026 |
| Spider Lift Scotland Limited | Other services | 2023 |
| Spirit + Structure Architecture Ltd | Professional & scientific | 2024 |
| Spot a Plot Ltd | Households | 2024 |
| Spowart Associates Ltd | Professional & scientific | 2017 |
| Spray IT UP (Scotland) Ltd | Construction | 2025 |
| SR Roadmarking Ltd | Construction | 2019 |
| SRI Hari Hara Retail Ltd | Wholesale & retail | 2026 |
| SRI Vikram Medical Ltd | Health & social care | 2024 |
| Srxt MGT Limited | Professional & scientific | 2021 |
| Ssetic Limited | Wholesale & retail | 2010 |
| SSR Cars Ltd | Administrative & support | 2017 |
| ST Andrews Bespoke Luxury Tours Ltd | Administrative & support | 2026 |
| ST Margaret's Loft Apartment Ltd | Real estate | 2022 |
| ST Private Ltd | Real estate | 2019 |
| ST. John Engineering Limited | Professional & scientific | 2018 |
| Stag Design LLP | | 2013 |
| Stage and Screen Academies Limited | Education | 2021 |
| Staks Development Ltd | Construction | 2021 |
| Standalane Ltd | Agriculture & fishing | 2025 |
| Stania Solutions Ltd | Information & communication | 2021 |
| Star 84 Ltd | Hospitality | 2019 |
| Static Reality Ltd | Financial services | 2025 |
| Stay Dunfermline Properties Limited | Hospitality | 2018 |
| Steel Buildings International Ltd | Manufacturing | 2019 |
| Stephen Irwin Joinery Ltd | Construction | 2024 |
| Stephen Morrow Agencies Ltd. | Wholesale & retail | 2012 |
| Sterling Reward Management Limited | Professional & scientific | 2012 |
| Stevenson & Marshall LLP | | 2005 |
| Stevie Crawford Football Academy Limited | Arts & recreation | 2024 |
| Stewards Bistro Limited | Hospitality | 2003 |
| Stewarts Piping Limited | Professional & scientific | 2014 |
| Stickman Food Company Ltd | Hospitality | 2022 |
| Stiffy's Shots Limited | Manufacturing | 2001 |
| Stingray Logistics Limited | Transport & logistics | 2019 |
| Stirling Developments Limited | Real estate | 1999 |
| Stirling Water Seafield Finance PLC | Financial services | 1998 |
| Stirling Water Seafield Holdings Limited | Water & waste | 1998 |
| Stirling Water Seafield Limited | Water & waste | 1998 |
| STM Access Ltd | Administrative & support | 2017 |
| Stonehall Flint Ltd | Hospitality | 2026 |
| Stongbet Ltd | Wholesale & retail | 2026 |
| Stratasea Limited | Professional & scientific | 2025 |
| Strathbeg Estates Limited | Hospitality | 2019 |
| Strathclyde Cars Ltd | Wholesale & retail | 2019 |
| Stsi Ltd | Financial services | 2022 |
| Stuart Donaldson Testing Consultants Limited | Professional & scientific | 2017 |
| Stuart King Architecture & Design Ltd. | Professional & scientific | 2009 |
| Stuart RAE Ltd | Transport & logistics | 2025 |
| Studio 11 Dance Collective Limited | Education | 2023 |
| Stufi Properties Ltd | Construction | 2015 |
| Subdex Ltd | Professional & scientific | 2026 |
| Submax Performance Ltd | Wholesale & retail | 2013 |
| Submertec Limited | Manufacturing | 1989 |
| Sukhi Homes Ltd | Real estate | 2021 |
| Sultan Brothers Ltd | Hospitality | 2024 |
| SUM DIY Limited | Wholesale & retail | 2023 |
| Summer Nails & Spa Dunfermline Limited | Other services | 2024 |
| Summers Homes (Fife) Limited | Hospitality | 1998 |
| Summit Rides Ltd | Transport & logistics | 2025 |
| Sun Harvester Limited | Manufacturing | 2017 |
| Sunday’S Cafe Limited | Hospitality | 2018 |
| Sunshine Brite Ltd | Transport & logistics | 2025 |
| Suraya Properties Ltd | Real estate | 2024 |
| Survivability Consulting Limited | Professional & scientific | 2010 |
| Susanto and Family Ltd | Transport & logistics | 2026 |
| Sustainable Homes Scotland Ltd | Construction | 2007 |
| SVS Consulting Ltd | Information & communication | 2021 |
| SW Automotive (Scotland) Ltd | Wholesale & retail | 2012 |
| SW Carluke Limited | Hospitality | 2015 |
| SW Hamilton Limited | Hospitality | 2015 |
| SW Holdings (Scotland) Ltd | Real estate | 2016 |
| SW Oban Limited | Hospitality | 2015 |
| SW Properties (Scotland) Ltd | Real estate | 2016 |
| SW Uddingston Limited | Hospitality | 2015 |