| NBL Investment Properties Limited | Real estate | 2025 |
| Ncom Holdings Ltd | Financial services | 2026 |
| NDK Fife Ltd | Hospitality | 2023 |
| NDS (UK) Limited | Professional & scientific | 2004 |
| Neilly Surgical Limited | Health & social care | 2023 |
| Nelson Fleet Solutions Ltd | Wholesale & retail | 2025 |
| Nemesis Jauk Limited | Wholesale & retail | 2014 |
| Nessie Lets Ltd | Hospitality | 2019 |
| Netlatch Limited | Hospitality | 2001 |
| NEUFESTUNG1 Ltd | Construction | 2026 |
| Neurovault Technologies Ltd | Information & communication | 2025 |
| Never Ending Pawfection Ltd | Other services | 2022 |
| Never Sink Limited | Wholesale & retail | 2025 |
| New Form Architecture Ltd | Professional & scientific | 2019 |
| New Generation Homes Limited | Construction | 2011 |
| New Horizon Retail Ltd | Wholesale & retail | 2023 |
| New Oakley Ltd | Hospitality | 2026 |
| New Reality Counselling Ltd | Health & social care | 2025 |
| New ROW Bite and Delights Ltd | Wholesale & retail | 2025 |
| New ROW Hair and Beauty Ltd | Other services | 2022 |
| New Selecto Ltd | Wholesale & retail | 2024 |
| New-Found-Hope Ltd | Wholesale & retail | 2021 |
| Newcrest Residential Limited | Construction | 2011 |
| Newlands Care Limited | Health & social care | 2012 |
| Newpubcompanyone Limited | Hospitality | 2024 |
| Newpubcompanytwo Limited | Hospitality | 2025 |
| Nexoravibe Ltd | Wholesale & retail | 2026 |
| Nexorax Digital Ltd | Information & communication | 2026 |
| Next Invest Group Ltd | Financial services | 2025 |
| Next Level Performance Strength and Conditioning Limited | Education | 2021 |
| Nexum Construction Limited | Construction | 2026 |
| NG Investment Holdings Ltd | Financial services | 2024 |
| NH Mortgages Ltd | Financial services | 2018 |
| Nhbc Accounting and Tax Services Limited | Professional & scientific | 2016 |
| Niall K D Martin Ltd | Professional & scientific | 2024 |
| Nice and Handy Ltd | Construction | 2026 |
| Nicki Penman Hairdressing Ltd | Other services | 2018 |
| Nicky's Garage Ltd. | Wholesale & retail | 2017 |
| Nicola Plunkett Consultancy Limited | Administrative & support | 2023 |
| Nightscout Pro Ltd | Information & communication | 2023 |
| Ninaform Solutions Ltd | Wholesale & retail | 2023 |
| Nisa Dalgety Bay Ltd | Wholesale & retail | 2025 |
| Nisbet Properties Ltd | Real estate | 2021 |
| Nisbet Welding Services Ltd | Construction | 2024 |
| Nisha & Brotherss Ltd | Wholesale & retail | 2026 |
| Nitrafit Ltd | Arts & recreation | 2019 |
| Njordr Ltd | Hospitality | 2026 |
| NL Productions Limited | Administrative & support | 2014 |
| NM Bars Limited | Hospitality | 2018 |
| NMB Property Investments Ltd | Real estate | 2022 |
| NMI Music Ltd | Information & communication | 2026 |
| NN Restaurants Limited | Hospitality | 2017 |
| NO Bubbles Ltd | Information & communication | 2018 |
| Nobel Global Limited | Professional & scientific | 2008 |
| Nonesuch Metals Limited | Arts & recreation | 2018 |
| Nordicforce Ltd | Transport & logistics | 2026 |
| Normaco LP | | 2011 |
| Normand & Thomson (Hillend) Limited | Manufacturing | 1971 |
| North Larches Energy Ltd | Professional & scientific | 2023 |
| North Road Garage Limited | Wholesale & retail | 2003 |
| North Star Consulting and Research Ltd. | Professional & scientific | 2011 |
| North Star Property Holdings Limited | Administrative & support | 2018 |
| Northvault Holdings Ltd | Real estate | 2026 |
| Nosha Btafb Limited | Transport & logistics | 2019 |
| Noskah Ltd | Administrative & support | 2023 |
| NOT Fast and Furious Ltd | Education | 2024 |
| Novo Ches Projects Limited | Professional & scientific | 2023 |
| Novo Leadership Development Limited | Professional & scientific | 2015 |
| NP Scots Ltd | Hospitality | 2025 |
| NT Logistics Ltd | Transport & logistics | 2022 |
| NU Limits Running Ltd | Arts & recreation | 2024 |
| Nuance Healthcare Limited | Health & social care | 2013 |
| Nurah Fast Food Ltd | Hospitality | 2019 |
| Nushly Limited | Transport & logistics | 2019 |
| Nyla Estates Ltd | Real estate | 2024 |
| O&D Clinic Properties Ltd | Real estate | 2022 |
| O&M Engineering Ltd | Manufacturing | 2025 |
| O'Neill Motors MOT Centre Ltd | Wholesale & retail | 2023 |
| O'Neill Mtrs Ltd | Wholesale & retail | 2018 |
| O'Reilly Construction Scotland Ltd | Construction | 2015 |
| O'Reilly Windows and Doors Ltd | Construction | 2024 |
| Oakley Store Ltd | Other services | 2016 |
| Oasis Cloud Limited | Information & communication | 2018 |
| Odie Bear Ltd | Real estate | 2020 |
| Offcertsuk Ltd | Professional & scientific | 2024 |
| OFG Group Limited | Wholesale & retail | 2022 |
| OFM Property Ltd | Real estate | 2025 |
| Ogilvie Ross LLP | | 2016 |
| Ogilvies of Haddington Limited | Manufacturing | 2018 |
| Okto Group Holdings Ltd | Financial services | 2024 |
| Okto Media Ltd | Information & communication | 2022 |
| OLA Derm Clinic Ltd | Other services | 2021 |
| OLA Productions Limited | Professional & scientific | 2025 |
| Old Boozy COW Ltd | Hospitality | 2018 |
| Old Swan Gargrave Limited | Hospitality | 2025 |
| Old Town Barber Club Limited | Other services | 2020 |
| Oliver & Robb Architects LLP | | 2006 |
| Ollie Hooper Ltd | Professional & scientific | 2017 |
| Omnia IT Solutions Ltd | Information & communication | 2024 |
| ON CUE World Limited | Other services | 2023 |