| Infinity Commissioning Services Ltd | Professional & scientific | 2019 |
| Inkinda Ltd | Wholesale & retail | 2021 |
| Innitiative Ltd | Administrative & support | 2023 |
| Innovation Joinery and Building Services Ltd | Construction | 2020 |
| Inquisitive Mind Limited | Wholesale & retail | 2009 |
| Insperata Architecture Ltd | Professional & scientific | 2024 |
| Inspire Physio Ltd | Health & social care | 2014 |
| Inspirefit Limited | Arts & recreation | 2013 |
| Installer Direct Limited | Other services | 2021 |
| Installerhub Ltd | Information & communication | 2026 |
| Instrument Access Solutions Ltd | Professional & scientific | 2023 |
| Instrumentum Precision Limited | Professional & scientific | 2026 |
| INT Sports Consultancy Ltd | Arts & recreation | 2013 |
| Integra.Engineer Limited | Professional & scientific | 2025 |
| Integrity Social Care Solutions Ltd | Health & social care | 2017 |
| Intellicare Limited | Professional & scientific | 1996 |
| Intellilocate Limited | Professional & scientific | 2007 |
| Interflon Scotland Limited | Wholesale & retail | 2020 |
| Intermobile Solutions Ltd | Manufacturing | 2016 |
| International Aria Star Trade Limited | Wholesale & retail | 2022 |
| International Team Recruitment Ltd | Transport & logistics | 2022 |
| Internet Strategies (UK) Limited | Information & communication | 2000 |
| Inuous Systems Ltd | Manufacturing | 2022 |
| Invereen Ventures Limited | Information & communication | 2023 |
| Inverkeithing Highland Games | Arts & recreation | 1997 |
| Invescot Ltd | Administrative & support | 2025 |
| Invest India Limited | Financial services | 2010 |
| Investment ST Ltd | Real estate | 2020 |
| IOT Reps Limited | Wholesale & retail | 2022 |
| IRO Decorators Ltd | Construction | 2023 |
| Iron 40 Ltd | Arts & recreation | 2025 |
| Ironmill Media Ltd | Information & communication | 2025 |
| Ischia Limited | Extraterritorial | 2011 |
| Isee Opticians Ltd. | Wholesale & retail | 2011 |
| Ishaaq Property Ltd | Real estate | 2025 |
| Isla Temple Limited | Real estate | 2018 |
| Isle of Canty Kennels Limited | Agriculture & fishing | 2019 |
| Istry CIC | Arts & recreation | 2016 |
| IT Works (Scotland) Limited | Information & communication | 2006 |
| It's GOT Pockets Limited | Wholesale & retail | 2022 |
| Italian Sunrise Limited | Hospitality | 2011 |
| It’S Never TOO Late Events Ltd | Arts & recreation | 2023 |
| IVY Lounge Rosyth Limited | Other services | 2025 |
| IVY Wednesday Ltd | Real estate | 2024 |
| Izatt Brown Ltd | Hospitality | 2025 |
| Izon Projects Ltd | Construction | 2011 |
| J & J Plumbing and Heating (Fife) Ltd | Construction | 2023 |
| J & J Wylie (Pitconochie) | | 1999 |
| J & J Wylie (Pitcononchie) | | 2005 |
| J & N Wragg Ltd | Hospitality | 2024 |
| J & V Home Improvements Ltd | Construction | 2018 |
| J & V Property Maintenance Ltd | Administrative & support | 2018 |
| J a M Electrical Ltd | Construction | 2020 |
| J Bowman Property Ltd | Hospitality | 2025 |
| J D Bikes Limited | Professional & scientific | 2015 |
| J Duncan PHB Ltd | Construction | 2016 |
| J Hunkin Services Ltd | Information & communication | 2019 |
| J J Duncan Properties Limited | Real estate | 2022 |
| J L Lawlor Ltd | Hospitality | 2017 |
| J Lawson Plumbing & Heating Ltd | Construction | 2018 |
| J LOW Agri Services Ltd | Manufacturing | 2010 |
| J M Heeps Ltd | Construction | 2016 |
| J Montgomery Roofing Limited | Construction | 2012 |
| J Tero Tech Ltd. | Construction | 2007 |
| J&J Vassallo Limited | Health & social care | 2023 |
| J-Teq EMS Employees 2022 Limited | Professional & scientific | 2022 |
| J-Teq EMS Holdings Two Ltd | Financial services | 2021 |
| J.F. Delivery Services Ltd | Transport & logistics | 2026 |
| J.I. Properties (Scotland) Limited | Real estate | 2001 |
| J.R.Scott Ltd | Hospitality | 2024 |
| Jacana Ltd | Real estate | 2025 |
| Jack Investment Property Ltd | Real estate | 2025 |
| Jackson 3J Ltd | Real estate | 2025 |
| Jakes ENG Maintenance Ltd | Administrative & support | 2017 |
| Jakobs Transport Ltd | Transport & logistics | 2021 |
| James Massey Consulting Ltd | Professional & scientific | 2025 |
| James Moore Haulage & Construction Ltd | Construction | 2023 |
| James Philp Limited | Construction | 2017 |
| Jamieson Contracting (Holdings) Limited | Professional & scientific | 2003 |
| Jamieson Contracting Company Limited | Manufacturing | 1993 |
| Jamieson Contracting Group Limited | Professional & scientific | 2007 |
| Jamieson Decorators (Fife) Ltd | Construction | 2022 |
| Jamieson Electrical Limited | Construction | 2018 |
| Jamil Estate Limited | Real estate | 2024 |
| Jane Macdowall Ltd | Professional & scientific | 2018 |
| Janeks Couriers Ltd | Transport & logistics | 2023 |
| Janjanikpt Ltd | Arts & recreation | 2025 |
| Japanese House Ltd | Hospitality | 2022 |
| Jarvie's of ST Andrews Ltd | Hospitality | 2018 |
| JAS Detailing Ltd | Administrative & support | 2026 |
| JAS Distributions Ltd | Wholesale & retail | 2024 |
| Jasiway Trading Limited | Manufacturing | 2024 |
| Jasmine Thai Herbal and Spa Ltd | Health & social care | 2019 |
| Jaycee VA Limited | Administrative & support | 2015 |
| Jays Performance Ltd | Wholesale & retail | 2003 |
| Jaysov Ventures Limited | Administrative & support | 2019 |
| Jazzhands Academy Limited | Education | 2014 |
| JB Boxing (Coaching and Management) Ltd | Professional & scientific | 2025 |
| JB Scotland Limited | Hospitality | 2019 |
| JCD 871872 Ltd | Financial services | 2025 |