| 1 Two Kids Limited | Wholesale & retail | 2009 |
| 1005 Taxis Ltd. | Professional & scientific | 2007 |
| 1292 Taxis Ltd. | Professional & scientific | 2010 |
| 12THIRTY Ltd | Administrative & support | 2014 |
| 15 Chalmers Street Limited | Real estate | 2025 |
| 1745 Events Ltd | Hospitality | 2025 |
| 1884 Ltd | Hospitality | 2020 |
| 1ST 4 Security Ltd. | Households | 2001 |
| 1ST Double Glazing Doctor Scotbased Limited | Construction | 2004 |
| 2-SARDARS Ltd | Construction | 2025 |
| 224 Taxis Ltd | Professional & scientific | 2013 |
| 247 Tree Surgery Limited | Construction | 2008 |
| 269 Vegan Kitchen Ltd | Wholesale & retail | 2021 |
| 3 Bridges Vets Limited | Professional & scientific | 2020 |
| 328 Taxis Ltd. | Transport & logistics | 2015 |
| 365 Travel Ltd | Transport & logistics | 2015 |
| 3BROTHER Fife Limited | Hospitality | 2025 |
| 3D Barber Fife Ltd | Other services | 2026 |
| 3D Barber Rosyth Ltd | Other services | 2025 |
| 405 Taxis Ltd. | Transport & logistics | 2016 |
| 52 Management LLP | | 2025 |
| 592 Edin Ltd. | Professional & scientific | 2006 |
| 5DBUILDING Ltd | Construction | 2020 |
| 60607 Ltd | Information & communication | 2023 |
| 7171MHB Ltd | Real estate | 2026 |
| 758 Taxis Ltd. | Extraterritorial | 2006 |
| 97 South Ltd | Real estate | 2019 |
| 99 South Ltd | Hospitality | 2011 |
| 9CONNECTION Limited | Other services | 2019 |
| A & S Joiners Limited | Construction | 2022 |
| A a Macfarlane Associates Limited | Professional & scientific | 2012 |
| A Class Grill Ltd | Hospitality | 2023 |
| A J Bathrooms and Boilers Ltd | Construction | 2017 |
| A J Pritchard Ltd | Administrative & support | 2018 |
| A Mcaloon Mentoring Ltd | Other services | 2023 |
| A Plus Tutors Ltd | Information & communication | 2009 |
| A S Allan Limited | Professional & scientific | 2010 |
| A Sutherland Design Ltd | Professional & scientific | 2019 |
| A&A Consultancy Limited | Professional & scientific | 2015 |
| A. & G. Cairncross Limited | Wholesale & retail | 1974 |
| A.B. Cant Limited | Professional & scientific | 1964 |
| A.B. Johnsons Limited | Hospitality | 1984 |
| A.D Communications (Fife) Ltd | Extraterritorial | 2019 |
| A.E.T Home Improvements Ltd | Administrative & support | 2009 |
| A.J. Hornig Limited | Manufacturing | 1972 |
| A.J.R. Financial Services (Scotland) Ltd. | Financial services | 2003 |
| A.N.G Analysis Limited | Extraterritorial | 2015 |
| A.S. Crawford Holdings Limited | Financial services | 2008 |
| A.W. Edwards Joiners Ltd | Administrative & support | 2017 |
| A1 Storage Lothian Limited | Administrative & support | 2016 |
| A100 Limited | Administrative & support | 1997 |
| AA Holidays Letting Ltd | Hospitality | 2018 |
| AA Tax Assist Ltd | Professional & scientific | 2025 |
| AA Vapour Limited | Wholesale & retail | 2018 |
| AAJ Estates Ltd | Real estate | 2019 |
| Aarkia Property Limited | Real estate | 2026 |
| Aaroha Homes Limited | Real estate | 2024 |
| Aawh Limited | Real estate | 2024 |
| AB Best Windows Ltd | Wholesale & retail | 2014 |
| AB Clinical Aesthetics Ltd | Other services | 2026 |
| AB Contracts Joinery Services Ltd | Construction | 2026 |
| AB17 Electronic Design Limited | Professional & scientific | 2016 |
| Abba Contractors Ltd | Construction | 2026 |
| Abbey Forth Property Management Ltd. | Other services | 2008 |
| Abbey Social Limited | Administrative & support | 2024 |
| Abbey Taxis (Dunfermline) Ltd | Transport & logistics | 2021 |
| Abbeyview Bowling Club | Arts & recreation | 2016 |
| Abbeyview Kids Club Limited | Other services | 1998 |
| Abbot House Trading Limited | Wholesale & retail | 2020 |
| Abbotshall Consulting Ltd | Professional & scientific | 2023 |
| Abbott Properties Limited | Real estate | 2005 |
| ABC Building & Developments Ltd | Construction | 2022 |
| ABC Supply Ltd | Wholesale & retail | 2025 |
| Aberdeen Gastroenterology Limited | Health & social care | 2021 |
| Aberfeldy Garage Ltd | Wholesale & retail | 2024 |
| Abhoster Ltd | Information & communication | 2026 |
| ABM Aesthetics & Beauty Limited | Other services | 2023 |
| Abon Homes Ltd | Real estate | 2026 |
| Abrush Ltd | Hospitality | 2023 |
| Absalute Resin Ltd | Administrative & support | 2024 |
| Absolute Floorcare Limited | Administrative & support | 2017 |
| Absolutely HR Ltd | Administrative & support | 2012 |
| Abuzar CH 2 Limited | Wholesale & retail | 2025 |
| Abyaan Ltd | Wholesale & retail | 2020 |
| Accent Gaming (Dunfermline) Ltd | Wholesale & retail | 2022 |
| Access Technical Ltd | Construction | 2025 |
| Accolade Heating Technologies Limited | Professional & scientific | 2004 |
| Accountancy Support Department Ltd | Professional & scientific | 2015 |
| Ace Alterations Ltd | Wholesale & retail | 2022 |
| Ace of Fades Fife Ltd | Other services | 2025 |
| ACF Engineering Ltd | Manufacturing | 2017 |
| Achieve Plumbing,Heating & Renewables Ltd | Construction | 2026 |
| ACJ Joinery Ltd | Construction | 2016 |
| ACR Electrical (Fife) Ltd | Manufacturing | 2020 |
| ACV U.K. Limited | Wholesale & retail | 1991 |
| AD Access Ltd | Construction | 2026 |
| AD Astra Assets Ltd | Real estate | 2022 |
| AD Smart Solutions Ltd | Information & communication | 2020 |
| Adam Link Ltd | Arts & recreation | 2019 |
| Adam M Welding Services Ltd | Manufacturing | 2021 |