| Novus Navitas Ltd. | Professional & scientific | 2004 |
| Novusity Ltd | Administrative & support | 2025 |
| NP Nails Ltd | Wholesale & retail | 2024 |
| NPC Advisory Limited | Professional & scientific | 2014 |
| NPD Installations Ltd | Construction | 2018 |
| NQB Dental Ltd | Health & social care | 2024 |
| NRB Inspections Limited | Construction | 2025 |
| Nrem Holdings Limited | Hospitality | 2019 |
| NRS Procurement Ltd | Administrative & support | 2013 |
| NS Property Services Ltd | Hospitality | 2023 |
| NSL (Fife) Limited | Hospitality | 2025 |
| NSV (Fife) Ltd | Hospitality | 2025 |
| NT Joinery Limited | Manufacturing | 2023 |
| NT Repairs Dundee Ltd | Wholesale & retail | 2026 |
| NTG Limited | Construction | 2016 |
| Ntoc Catering Ltd | Hospitality | 2024 |
| NUB Developments Limited | Construction | 2015 |
| Nudge Solutions Ltd | Wholesale & retail | 2020 |
| Number 21 Beauty Ltd | Other services | 2013 |
| Number 8 Real Estate Ltd | Real estate | 2022 |
| Number 8 Wealth Ltd | Real estate | 2022 |
| NUMBER14 Consulting Ltd. | Professional & scientific | 2012 |
| Nunatak Ltd | Professional & scientific | 2013 |
| Nutranatureco Limited | Wholesale & retail | 2023 |
| Nutrient Partitioning Ltd | Hospitality | 2024 |
| Nutrio Physiotherapy & Pilates Limited | Health & social care | 2025 |
| NWF Fabrication Ltd | Manufacturing | 2019 |
| NWR Electrical Perth Limited | Construction | 2018 |
| NXD Holdings Limited | Real estate | 2024 |
| NXD Properties Limited | Real estate | 2017 |
| NXT Level Builders Limited | Construction | 2025 |
| Nyala Clothing Limited | Wholesale & retail | 2024 |
| Nyxia Ltd | Wholesale & retail | 2025 |
| NZ Electronics Ltd | Wholesale & retail | 2024 |
| Nzone Fincas Ltd | Real estate | 2022 |
| Nzuko NDI Igbo Dundee CIC | Education | 2025 |
| O&O Fabrication Ltd | Manufacturing | 2025 |
| O'Reilly Safety Services Limited | Professional & scientific | 2018 |
| O3ST Limited | Professional & scientific | 2021 |
| O@K Window Cleaning / Maintenance Limited | Administrative & support | 2024 |
| OA Labs Ltd | Professional & scientific | 2010 |
| OAG Electrical Limited | Construction | 2014 |
| Oak Builders Direct Ltd | Construction | 2020 |
| Oakfell Limited | Administrative & support | 2023 |
| Oakstrong Limited | Hospitality | 1998 |
| Oats Business Consultancy Limited | Administrative & support | 2007 |
| Oats Trading Limited | Wholesale & retail | 2012 |
| OB1 Control Solutions Ltd | Professional & scientific | 2022 |
| OB1 Productions Ltd | Information & communication | 2023 |
| OBA ILE Ileri ( God's Promise Land) Community Interest Company | Other services | 2023 |
| Oban Caravan & Camping Park Limited | Hospitality | 2002 |
| Oban Joinery Services Limited | Construction | 2011 |
| Obar 23 Ltd | Real estate | 2023 |
| Obiz Global Services Limited | Transport & logistics | 2026 |
| Objective Associates Limited | Information & communication | 2002 |
| OCC Cleaning Services Ltd | Administrative & support | 2017 |
| Occam Biosciences Limited | Professional & scientific | 2021 |
| Occasional Furniture (Manufacturing) Limited | Water & waste | 1987 |
| Occasionist Limited | Wholesale & retail | 2019 |
| Occasions Leisure Ltd | Hospitality | 2019 |
| Occca Limited | Wholesale & retail | 2025 |
| Occi Ferry Ltd | Hospitality | 2025 |
| Occo Jewels Ltd | Wholesale & retail | 2026 |
| Ocean Catering Ltd | Hospitality | 2010 |
| Ocean Transport and Trading L.P. | | 2015 |
| Oceane Subsea Ltd | Administrative & support | 2011 |
| Oceanteam Associates Limited | Professional & scientific | 2015 |
| Ocelle Ltd | Information & communication | 2025 |
| Ocelot Craft Chocolate Limited | Manufacturing | 2022 |
| Octoza Ltd | Information & communication | 2018 |
| Odds & Insights Ltd | Professional & scientific | 2019 |
| Odella Invest LP | | 2017 |
| Odin Consultancy & Recruitment Ltd | Information & communication | 2016 |
| Odolis Trade LP | | 2015 |
| OFF Grid Travel Limited | Administrative & support | 2019 |
| Office Direct Aberdeen Limited | Manufacturing | 2011 |
| OFFICE26 Limited | Administrative & support | 2023 |
| Offshore Environmental Oils Limited | Manufacturing | 2012 |
| Offshore Management Limited | Professional & scientific | 2010 |
| Offshore Scaffold Inspection Services Ltd | Construction | 2008 |
| Offspring Property Limited | Real estate | 2025 |
| OG Heating Services Ltd | Construction | 2013 |
| OG Lettings Ltd | Real estate | 2022 |
| Ogilvie Advisory Ltd | Professional & scientific | 2016 |
| OGM (One) Limited | Hospitality | 2018 |
| Ogre Games Limited | Information & communication | 2019 |
| Ohso Gorgeous Hair Academy Ltd | Other services | 2019 |
| OIL & GAS Dundee Ltd | Professional & scientific | 2017 |
| Oilfield International Services Ltd | Professional & scientific | 2012 |
| OJ Games Ltd | Information & communication | 2025 |
| OJE Studios Ltd. | Information & communication | 2023 |
| OK Corral Ltd | Hospitality | 2019 |
| OK Dokey Limited | Construction | 2025 |
| Okay Dino Games Limited | Information & communication | 2024 |
| Okhai Properties Limited | Real estate | 2025 |
| Olaf Postola Limited | Professional & scientific | 2022 |
| Old Hawkhill Bars Ltd | Hospitality | 2024 |
| Old Manse Properties Limited | Administrative & support | 2016 |
| Old Mill Joiners Limited | Construction | 2025 |
| Old Pico Limited | Construction | 2017 |