| Fort ST Consultants Ltd | Information & communication | 2025 |
| Fort Street Motors Ltd | Wholesale & retail | 2009 |
| Fortcastle Trade L.P. | | 2014 |
| Fortesk Ltd | Administrative & support | 2015 |
| Forth Care Limited | Health & social care | 2013 |
| Forth Valley Travel Ltd | Transport & logistics | 2018 |
| Forthill Investments Ltd | Information & communication | 2017 |
| Forthill SC Limited | Hospitality | 2015 |
| Forthview Digital Ltd | Information & communication | 2024 |
| Forthview Ltd | Professional & scientific | 2021 |
| Fortlex Import L.P. | | 2015 |
| Fortpoint Trans L.P. | | 2015 |
| Fortrade Export L.P. | | 2015 |
| Fortwing Capital L.P. | | 2012 |
| Foster Electrical (Scotland) Limited | Construction | 2024 |
| Foster Plumbing & Heating Limited | Construction | 2025 |
| Fostering People Scotland Limited | Health & social care | 2012 |
| Fotheringham Property Limited | Real estate | 2025 |
| Fouin & Bell Architects Limited | Professional & scientific | 1999 |
| Foundmark Nordic L.P. | | 2015 |
| Foundry Investments Limited | Real estate | 2019 |
| Fountainhall Consultants Ltd | Professional & scientific | 2014 |
| Four Queen Street Limited | Hospitality | 2025 |
| Four Seasons Catering and Bar MGC Ltd | Hospitality | 2025 |
| Fourm Accounting Services Limited | Professional & scientific | 2010 |
| Fourm Limited | Extraterritorial | 2015 |
| Fowlis Contracts Limited | Construction | 2016 |
| FOX and Manō Ltd | Manufacturing | 2025 |
| FOX HAT Ltd | Hospitality | 2017 |
| Foxdale Products L.P. | | 2015 |
| Foxmark Universal L.P. | | 2016 |
| Foyzer Services Limited | Mining & quarrying | 2018 |
| FR Couriers Ltd | Transport & logistics | 2026 |
| Frances Hunter Hair Limited | Other services | 2018 |
| Frances Hunter Holdings LLP | | 2018 |
| Frances Pipe Fitting Limited | Administrative & support | 2017 |
| Franchhoek Consulting Ltd | Professional & scientific | 2010 |
| Francostar Properties L.P. | | 2016 |
| Frank Kelbie Limited | Wholesale & retail | 1991 |
| Frank Milne Limited | Professional & scientific | 2022 |
| Franklin Decorators Limited | Construction | 2026 |
| Franks Auto Centre Ltd | Wholesale & retail | 2004 |
| Franks Holdings Dundee Limited | Financial services | 2022 |
| Franks Trading Limited | Hospitality | 2022 |
| Fraoch Northfield Limited | Administrative & support | 2011 |
| Fraser & Michelle Property Ltd | Real estate | 2023 |
| Fraser Engineering (Scotland) Ltd | Professional & scientific | 2024 |
| Fraser Robertson Flooring Ltd | Construction | 2026 |
| Fraser's Fruit and VEG Limited | Wholesale & retail | 2015 |
| Frazerhair Limited | Other services | 2025 |
| Frazerinternational Limited | Other services | 2025 |
| Freedom Dog Park Limited | Agriculture & fishing | 2023 |
| Freedom Financial Limited | Financial services | 2018 |
| Freedom Lettings Ltd | Real estate | 2019 |
| Freedom Real Estate Holdings Limited | Hospitality | 2017 |
| Freeinvest L.P. | | 2013 |
| Freelance Energy Services Limited | Professional & scientific | 2004 |
| Freelance Euro Services (Mmccclxxxiv) Limited | Information & communication | 2006 |
| Freelance Euro Services (Mmcxvii) Limited | Administrative & support | 2006 |
| Freelance Fabrication Ltd | Construction | 2024 |
| Freemark Networks LP | | 2015 |
| Freepoint Impex L.P. | | 2012 |
| Freeport Energy L.P. | | 2013 |
| Frelat Limited | Professional & scientific | 2012 |
| Frendraught Capital Limited | Real estate | 2016 |
| Fresh Steps Carpet Care Ltd | Administrative & support | 2026 |
| Fresh-Jet Catering Limited | Hospitality | 2007 |
| Freya's Wish C.I.C. | Wholesale & retail | 2021 |
| Frieed Chicken Ltd | Hospitality | 2026 |
| Friendly Wheels Dundee Limited | Transport & logistics | 2025 |
| Frisco Project Management Consultancy Services Limited | Manufacturing | 2017 |
| Front Lounge Limited | Administrative & support | 2001 |
| Front ROW Lettings Ltd | Real estate | 2020 |
| Frontera Investments Limited | Financial services | 2018 |
| Frontline Training Group Ltd | Extraterritorial | 2020 |
| Frontstone Commercial L.P. | | 2015 |
| Frontway Trans L.P. | | 2015 |
| Frood Rope Access Ltd | Mining & quarrying | 2024 |
| Frozer.Pk Ltd | Wholesale & retail | 2026 |
| Frui Vita Ltd | Real estate | 2017 |
| Frunchii Ltd | Wholesale & retail | 2025 |
| Fryer Moving & Storage Ltd | Transport & logistics | 2018 |
| Frying Scotsman Ltd | Hospitality | 2025 |
| FS Ward Limited | Real estate | 2006 |
| FS92 Ltd | Transport & logistics | 2024 |
| Fsha Investments Limited | Real estate | 2024 |
| FSM Auto Services Ltd | Wholesale & retail | 2024 |
| FSM Engineering Ltd | Professional & scientific | 2017 |
| Fspkss Ltd | Construction | 2013 |
| FT Corporate Services Ltd | Professional & scientific | 2019 |
| FT Emerald Ltd | Construction | 2026 |
| Fuel FIX (Dundee) Ltd | Hospitality | 2024 |
| FUJ Properties Limited | Real estate | 2023 |
| Full Circle Bakes Ltd | Wholesale & retail | 2021 |
| Fuller Thomson Heritable Limited | Hospitality | 2003 |
| Fullerton Cooper Legal Ltd | Professional & scientific | 2021 |
| Fullerton Cooper Solutions Ltd | Other services | 2021 |
| Functional Fitness Dundee Limited | Administrative & support | 2013 |
| Functional Fitness Kids Limited | Arts & recreation | 2016 |
| Functional Fitness ST. Andrews Limited | Arts & recreation | 2015 |