| Dundee Ride Ltd | Transport & logistics | 2025 |
| Dundee Road Ltd | Wholesale & retail | 2025 |
| Dundee Roller Derby Ltd | Arts & recreation | 2015 |
| Dundee Rugby Club Ltd. | Arts & recreation | 2017 |
| Dundee Safety Training Services Limited | Other services | 2014 |
| Dundee Satellite Station Ltd | Information & communication | 2019 |
| Dundee Scaffolding Solutions Ltd | Construction | 2022 |
| Dundee Science Centre | Administrative & support | 1997 |
| Dundee Science Centre Enterprises Limited | Education | 1998 |
| Dundee Shawarma House Ltd | Hospitality | 2026 |
| Dundee Social Enterprise Network | Other services | 2014 |
| Dundee Soft Play & Bounce Hire Ltd | Arts & recreation | 2025 |
| Dundee Sports Medicine Limited | Health & social care | 2025 |
| Dundee Strength Unit Ltd | Arts & recreation | 2017 |
| Dundee Supplies Ltd | Wholesale & retail | 2026 |
| Dundee Sweets Ltd | Hospitality | 2023 |
| Dundee Symphony Orchestra Production Ltd. | Arts & recreation | 2025 |
| Dundee Taksi Ltd | Transport & logistics | 2025 |
| Dundee Taxis Ltd | Transport & logistics | 2011 |
| Dundee TAY Street Ltd | Hospitality | 2026 |
| Dundee Tech Store Ltd | Wholesale & retail | 2026 |
| Dundee Travels Ltd | Transport & logistics | 2022 |
| Dundee Tribe Cheerleaders CIC | Education | 2025 |
| Dundee Tyre Shop Ltd | Wholesale & retail | 2024 |
| Dundee United Community Trust | Arts & recreation | 2008 |
| Dundee United Supporters Foundation Limited | Other services | 2017 |
| Dundee Volunteer and Voluntary Action Ltd | Other services | 1985 |
| Dundee Watch Company Ltd | Manufacturing | 2016 |
| Dundee Women's AID Company Limited | Hospitality | 1990 |
| Dundee Youth Initiative Community Interest Company | Health & social care | 2024 |
| Dundee Youth Music Theatre Ltd | Education | 2022 |
| Dundeecleppyhouse Limited | Hospitality | 2025 |
| Dundeeropeheight Ltd | Transport & logistics | 2025 |
| Dundeewrapping Ltd | Professional & scientific | 2019 |
| Dunella Ltd | Professional & scientific | 2025 |
| Dunholm Improvements Ltd | Construction | 2020 |
| Dunholm Solid Fuel Services Ltd | Construction | 2024 |
| Dunia Organic Ltd | Wholesale & retail | 2018 |
| Dunkeld Care Ltd | Health & social care | 2017 |
| Dunkeld Hotel Limited | Hospitality | 2020 |
| Dunlunan Joinery Limited | Construction | 2024 |
| Dunmar Electrical Ltd | Construction | 2014 |
| Dunndee GAS Limited | Energy | 2024 |
| Dunndee Property Limited | Real estate | 2017 |
| Dunrow Properties Limited | Real estate | 2023 |
| Dunshelt Investments Limited | Professional & scientific | 1991 |
| Dunsinane Consultants Limited | Extraterritorial | 2016 |
| Duntrune Consulting Limited | Other services | 2018 |
| Dunwick Surveys Limited | Construction | 2002 |
| Durie Street Developments Ltd | Construction | 2018 |
| Dusting Donna Limited | Administrative & support | 2019 |
| Duthie Joinery Xylo Limited | Construction | 2026 |
| Dutsen Kauna Global Resources UK Ltd | Wholesale & retail | 2021 |
| Duxi Limited | Wholesale & retail | 2015 |
| DV Taxis Ltd | Transport & logistics | 2020 |
| DV8 (Dundee) Ltd | Wholesale & retail | 2021 |
| DW Hair Aesthetics Ltd | Other services | 2025 |
| DWK Office Solutions Limited | Wholesale & retail | 2003 |
| DWK Outdoor Living Ltd | Construction | 2021 |
| DX Universal L.P. | | 2016 |
| Dyford Overseas L.P. | | 2015 |
| Dymock Properties Ltd | Real estate | 2022 |
| Dynamic Drilling Consultants Ltd | Administrative & support | 2015 |
| Dynamic Partners L.P. | | 2014 |
| Dynamic Retail Ltd | Wholesale & retail | 1997 |
| Dyslexia & Literacy Support Services Limited | Education | 2026 |
| E & E Rope Access Ltd | Professional & scientific | 2018 |
| E & I Services (Dundee) Limited | Construction | 2011 |
| E & S Carnoustie Ltd | Real estate | 2022 |
| E Egan Architect & Surveyors Ltd | Professional & scientific | 2013 |
| E Trade & Accounts Hub Ltd | Administrative & support | 2026 |
| E&H Food Ltd | Hospitality | 2024 |
| E-Matters Consulting Limited | Information & communication | 2007 |
| E-Technic Ltd | Manufacturing | 2021 |
| E.C.E.Engineering (Dundee) Limited | Manufacturing | 1978 |
| E.C.S. Dundee Limited | Construction | 2020 |
| E.J.F. Couriers Ltd | Transport & logistics | 2021 |
| E.N. Recruitment Services Ltd | Administrative & support | 2008 |
| E.P. Engineering Company (Dundee) Limited | Manufacturing | 1978 |
| E.Tosh Logistics Limited | Transport & logistics | 2025 |
| E33 Restaurants Ltd | Hospitality | 2024 |
| EA Aerostructures Ltd. | Manufacturing | 2016 |
| Eagas Consulting Ltd | Professional & scientific | 2024 |
| Eaglebay Limited | Real estate | 1995 |
| Earl Grey Tattoo Ltd | Arts & recreation | 2021 |
| Earl's Rest Property Limited | Real estate | 2023 |
| Earlcross Logistics L.P. | | 2014 |
| Earline Accents LP | | 2017 |
| Earlish Services Limited | Real estate | 2003 |
| Early Birds Properties Ltd | Real estate | 2023 |
| Earthmover Tyres (Scotland) Limited | Manufacturing | 1999 |
| Easemovez Ltd | Transport & logistics | 2025 |
| East Coast Combustion Services Limited | Construction | 1980 |
| East Coast Drainage Solutions Limited | Construction | 2009 |
| East Coast Fitout Ltd | Construction | 2024 |
| East Coast Inventories Limited | Real estate | 2025 |
| East Coast Krav Maga Scotland Ltd | Arts & recreation | 2026 |
| East Coast Projects Limited | Construction | 2019 |
| East Coast Quarry Materials and Commercial Consultants Ltd | Mining & quarrying | 2019 |
| East Coast Rendering Specialists Ltd | Construction | 2017 |