| 101 Dimensions Limited | Financial services | 2009 |
| 111 Locum Service Ltd | Health & social care | 2021 |
| 1313 Media Ltd | Wholesale & retail | 2024 |
| 24HS BOA Ltd | Hospitality | 2023 |
| 28TWELVE Investments Ltd | Real estate | 2022 |
| A & S Property Holdings Ltd | Real estate | 2019 |
| A G Donnan Ltd | Manufacturing | 2019 |
| A G Smith Accountancy Ltd | Professional & scientific | 2019 |
| A.D. Norman Ltd. | Professional & scientific | 1995 |
| Aaron Ferguson Ltd. | Agriculture & fishing | 2012 |
| Absolute Accounts Limited | Professional & scientific | 2024 |
| Accounting Business Services (Scotland) Limited | Professional & scientific | 2008 |
| Ad-Hoc Coaching Ltd | Education | 2026 |
| Addwell Scotland Ltd | Professional & scientific | 2023 |
| Adeptic Ltd | Information & communication | 2012 |
| Afaraed Ltd | Education | 2021 |
| Agre Property Ltd | Construction | 2026 |
| AIM Coaches (Scotland) Ltd | Transport & logistics | 2020 |
| AJB Racing Ltd | Arts & recreation | 2019 |
| AJD Advisory Ltd | Professional & scientific | 2018 |
| AJD1040 Ltd | Arts & recreation | 2016 |
| Alastair Forbes Architects Limited | Professional & scientific | 2020 |
| Albany AI Limited | Information & communication | 2024 |
| Alex R.R Hospitality Solutions Ltd | Hospitality | 2025 |
| Alexander Enterprises Limited | Administrative & support | 2008 |
| ALF Residential Limited | Real estate | 2025 |
| Alif Partners Limited | Professional & scientific | 2024 |
| Allanview Windows and Doors Ltd. | Wholesale & retail | 2017 |
| Ally Baird Ltd. | Construction | 2005 |
| ALM Catering Limited | Hospitality | 2017 |
| Almass Ltd | Other services | 2018 |
| Amasset Investments Limited | Real estate | 2019 |
| Amcounting Limited | Real estate | 2014 |
| Amcounting Services Limited | Professional & scientific | 2020 |
| AME Developments (Scotland) Ltd | Construction | 2018 |
| AMV Investments Ltd | Real estate | 2023 |
| Amwcs Limited | Administrative & support | 2013 |
| Angela Fraioli Limited | Professional & scientific | 2007 |
| Anne Maccoll Consulting Ltd. | Professional & scientific | 2016 |
| Aquaforce Limited | Administrative & support | 2021 |
| Arbeth Management Ltd. | Mining & quarrying | 2011 |
| Ardleighton Consulting Ltd | Professional & scientific | 2018 |
| Ardoch Development Trust | Other services | 2007 |
| Ardoch Holdings Ltd | Wholesale & retail | 2026 |
| Arthouse Apartments Limited | Real estate | 2020 |
| Arthur Property Developments Ltd | Real estate | 2024 |
| Artiz Retail Ltd | Hospitality | 2021 |
| ASF Services Limited | Mining & quarrying | 2019 |
| Atlas Project Services Limited | Professional & scientific | 2023 |
| Atlas Resources Ltd | Professional & scientific | 2019 |
| ATM Leisure Ltd | Hospitality | 2018 |
| Auchineden Limited | Agriculture & fishing | 1991 |
| Avdail LLP | | 2021 |
| Avenuehead Land Ltd | Construction | 2016 |
| Axiom Scientific Ltd | Professional & scientific | 2025 |
| B & L Properties (Scotland) Limited | Real estate | 2001 |
| Balformo Developments Limited | Hospitality | 1991 |
| Balhaldie Energy Limited | Energy | 2012 |
| Barnes OWL Ltd | Professional & scientific | 2020 |
| Bathregen Ltd | Construction | 2023 |
| Batleur Limited | Energy | 2018 |
| BD Legacy Ltd | Real estate | 2022 |
| Bell Rail Ltd | Professional & scientific | 2023 |
| Biadh Ltd | Administrative & support | 2025 |
| Big Galah Hospitality Company Ltd | Hospitality | 2023 |
| Birrell Consulting Limited | Professional & scientific | 2010 |
| Black Card Hospitality Company Ltd. | Hospitality | 2013 |
| Black Card Properties Ltd. | Real estate | 2014 |
| Blackbird Ventures Ltd | Financial services | 2025 |
| Boma Energy Limited | Energy | 2014 |
| Bonmotus Limited | Health & social care | 2022 |
| Bonnie Tartan Limited | Manufacturing | 2013 |
| Bonnybridge Angling Club | Arts & recreation | 2009 |
| Bonsyde Properties Ltd | Real estate | 2013 |
| Bonza Bars Bofa Ltd | Hospitality | 2022 |
| Bonza Bars STG Ltd | Hospitality | 2023 |
| Borthwick Properties Ltd | Hospitality | 2011 |
| Braco Castle Farms Limited | Agriculture & fishing | 1997 |
| Braco Wind Limited | Energy | 2012 |
| Brambling Finch Ltd | Wholesale & retail | 2023 |
| Bridge of Allan Investments Limited | Real estate | 2016 |
| Bridge of Allan Office Suites Limited | Real estate | 2018 |
| Brilliant Futures (Scotland) Ltd | Other services | 2010 |
| Brown Bagpipes Ltd | Manufacturing | 2026 |
| Buntine Limited | Hospitality | 2025 |
| Business Awards Events Ltd | Other services | 2024 |
| Buttery Flaky Crust Ltd | Professional & scientific | 2025 |
| C S Wilson Ltd | Information & communication | 2022 |
| C&H Investment Group Ltd | Real estate | 2019 |
| C4STORE Ltd | Wholesale & retail | 2025 |
| Caber Energy Limited | Administrative & support | 2010 |
| Cactus Black Ltd | Other services | 2022 |
| Cadha IT Solutions Limited | Information & communication | 2005 |
| Caife Ltd | Administrative & support | 2025 |
| Caitle Ltd | Administrative & support | 2025 |
| Caladail Ltd | Real estate | 2021 |
| Caledon Studio LLP | | 2025 |
| Caledonian Aircraft Spares Limited | Wholesale & retail | 1992 |
| Caledonian Christmas Trees Ltd | Agriculture & fishing | 2016 |
| Capital Events Edinburgh Ltd | Hospitality | 2018 |