| Greenleaf Global Group Co Limited | Financial services | 2018 |
| Greenleaf House Co Limited | Other services | 2018 |
| Greenleaf Prop Co Limited | Real estate | 2018 |
| Gretna Enterprise Ltd | Real estate | 2019 |
| Gretna Green Weddings Limited | Other services | 2010 |
| Greybe Fine Olive Products (UK) Ltd. | Wholesale & retail | 2016 |
| Grierson & Graham Limited | Wholesale & retail | 1956 |
| Grnc Consulting Limited | Administrative & support | 2023 |
| Grossick Racing Photography Limited | Professional & scientific | 2003 |
| Groszek International Ltd | Wholesale & retail | 2023 |
| Grow IT (Scotland) Limited | Agriculture & fishing | 2020 |
| Growing Herd Limited | Agriculture & fishing | 2025 |
| GRT Machinery Limited | Wholesale & retail | 2014 |
| GS Plumbing Limited | Construction | 2025 |
| GSM Innovations Ltd | Professional & scientific | 2024 |
| GTR Contracts Limited | Agriculture & fishing | 2006 |
| Guild of Players | Arts & recreation | 2012 |
| GWW Holdings Ltd | Financial services | 2024 |
| H & S Farms | | 1995 |
| H R Contractors Limited | Agriculture & fishing | 2005 |
| H&W Training Ltd | Education | 2023 |
| Haining Catering Limited | Hospitality | 2006 |
| Hair by Cheryl L Ltd | Other services | 2026 |
| Hair Style Dumfries Ltd | Other services | 2018 |
| Hairworks Dumfries Limited | Other services | 2013 |
| Halex Engineering Ltd | Manufacturing | 2021 |
| Halls Direct Limited | Wholesale & retail | 2013 |
| Hammer & Thistle Building and Design Ltd | Construction | 2017 |
| HANNAHSOLUTION25 Ltd | Manufacturing | 2025 |
| Happy Feet (UK) Distributing Limited | Wholesale & retail | 2010 |
| Happy Feet (UK) Limited | Wholesale & retail | 2013 |
| Hardgrave Farm, (Dumfries) Limited | Agriculture & fishing | 1954 |
| Hardgrove Farms Ltd | Agriculture & fishing | 2025 |
| Hardie Holdings Limited | Administrative & support | 2001 |
| Hardie Maclean Ltd | Financial services | 2020 |
| Hardie Studios Ltd | Administrative & support | 2015 |
| Harmony Thai Spa Ltd | Health & social care | 2025 |
| Harry Soames Limited | Professional & scientific | 2021 |
| Harwood Hunter Limited | Hospitality | 2026 |
| Haslam-Jones Marine Consultancy LLP | | 2018 |
| Hastings Bespoke Ltd | Manufacturing | 2018 |
| Haulage Express Limited | Transport & logistics | 1955 |
| Hawick Media Limited | Information & communication | 2026 |
| Hazels Helping Hands Ltd | Health & social care | 2025 |
| HB 100 Limited | Real estate | 2017 |
| HDL Nail Spa Ltd | Other services | 2026 |
| Headprint Ltd | Professional & scientific | 2026 |
| Hearing Supplies Limited | Wholesale & retail | 2026 |
| Hearth to Home Ltd | Construction | 2025 |
| Heath Hall Convenience Store Limited | Wholesale & retail | 2019 |
| Heathhall Business Centre Limited | Administrative & support | 2007 |
| Heathhall Holdings Limited | Financial services | 2016 |
| Heating Care Dumfries Ltd | Construction | 2023 |
| HELPINGHAND2U Ltd | Administrative & support | 2020 |
| Henderson Commercial Painters Limited | Construction | 2015 |
| Henderson Commercial Shot Blasting Limited | Extraterritorial | 2019 |
| Hendomultidrop Ltd | Households | 2025 |
| Henwood Transport Limited | Transport & logistics | 2015 |
| Hestan Asset Finance Limited | Financial services | 2003 |
| Hi-Line Kitchens & Joinery Limited | Construction | 2013 |
| Hiddleston Contracts Limited | Agriculture & fishing | 2019 |
| Hiddleston Joiners Limited | Manufacturing | 2001 |
| Hideout Scot Ltd | Hospitality | 2019 |
| High Voltage Maintenance Solutions Limited | Construction | 2020 |
| Highland Hard Seltzer Ltd | Wholesale & retail | 2023 |
| Highland Insurance Brokers Ltd | Financial services | 2014 |
| Hill Construction Services Ltd | Construction | 2025 |
| Hillowton Eggs Ltd | Wholesale & retail | 2024 |
| Hillview Accounts Services Ltd | Professional & scientific | 2020 |
| Hodgson Forestry & Tree Services Limited | Agriculture & fishing | 2026 |
| Hoggs Equestrian Services Ltd | Agriculture & fishing | 2015 |
| Hoggs Leisure Ltd. | Agriculture & fishing | 2011 |
| Hole I' the WA' Ltd | Hospitality | 2016 |
| Holiday Scotland Limited | Hospitality | 2005 |
| Hollinger Creative Ltd | Information & communication | 2011 |
| Hollinger Investing Ltd | Financial services | 2021 |
| Holmes Health and Social Care Consultancy Limited | Health & social care | 2012 |
| Holywood Developments Limited | Construction | 2024 |
| Holywood Service Station Limited | Wholesale & retail | 2012 |
| Home Reboot Ltd | Other services | 2019 |
| Home Storage Solutions Ltd | Transport & logistics | 2020 |
| Homebee Limited | Real estate | 2017 |
| Homerenewables Scotland Ltd | Construction | 2013 |
| HOMES4HENS Rescue C.I.C. | Other services | 2017 |
| Honesty Produce Loyalty Care Services Ltd | Health & social care | 2021 |
| Hopper Records Ltd | Wholesale & retail | 2023 |
| Howat Electrical Contractors Limited | Construction | 2010 |
| Howats Properties Limited | Real estate | 2024 |
| Howitt Services Ltd | Professional & scientific | 2021 |
| Hownd Clothing Limited | Wholesale & retail | 2025 |
| Hubmaier Ltd. | Manufacturing | 2018 |
| Huntingdon Garage Limited | Wholesale & retail | 2005 |
| Huntingdon House Ltd | Real estate | 2024 |
| Huntlys Garage Limited | Wholesale & retail | 2022 |
| I Wells Consultancy Ltd | Professional & scientific | 2023 |
| I.J. Broll Limited | Construction | 2015 |
| Iain Jardine Racing Ltd | Arts & recreation | 2016 |
| Iain Macfadzean Limited | Professional & scientific | 2004 |
| IAN D.Archibald Limited | Construction | 1976 |
| IAN HAY & SON Heating & Plumbing Ltd | Construction | 2026 |