| Dumfries Saints C.I.C. | Arts & recreation | 2016 |
| Dumfries Slow Fashion Movement CIC | Education | 2021 |
| Dumfries Smile Centre Ltd | Health & social care | 2025 |
| Dumfries Smile Studio Ltd | Health & social care | 2026 |
| Dumfries Technical Services Limited | Professional & scientific | 2012 |
| Dumfries Tool Room Limited | Manufacturing | 1993 |
| Dumfries Trade Ltd | Other services | 2026 |
| Dumfries Training Services Limited | Other services | 2002 |
| Dumfries Tyres and Autocare Ltd | Wholesale & retail | 2026 |
| Dumfries Van Hire Ltd | Administrative & support | 2024 |
| Dumfries Window Systems Limited | Construction | 2015 |
| Dumfriesminimarket Limited | Wholesale & retail | 2023 |
| Dumfriesshire & Stewartry Women's AID | Other services | 1993 |
| Dunbar Race Engineering Ltd | Professional & scientific | 2017 |
| Dundas Chemical Company (Mosspark) Limited | Manufacturing | 1958 |
| Dundas Transport 1 Ltd | Financial services | 2020 |
| Dundas Transport Ltd | Transport & logistics | 2020 |
| DW Organics Ltd | Manufacturing | 2025 |
| DWS Contracts Limited | Administrative & support | 2024 |
| Dynamic Domestic Ltd | Administrative & support | 2026 |
| Dynamic Ventures (Scotland) Limited | Professional & scientific | 2016 |
| E-Architect Limited | Information & communication | 2009 |
| Each Holdings Limited | Manufacturing | 2011 |
| Easy Read for YOU CIC | Professional & scientific | 2023 |
| Easy Shop Dumfries Limited | Wholesale & retail | 2024 |
| Easykey Mortgages Ltd | Financial services | 2025 |
| EB Metering Limited | Construction | 2026 |
| Ebenezer Stores Ltd | Wholesale & retail | 2024 |
| Eco-Lg Limited | Professional & scientific | 2021 |
| Eco-Solutions Scotland Ltd | Construction | 2024 |
| Ecoclean Solutions Ltd | Administrative & support | 2020 |
| Eden Utility Engineering Limited | Construction | 2016 |
| Edinburgh Hideaways Ltd | Hospitality | 2022 |
| Edinburgh Self Storage Containers Limited | Extraterritorial | 2017 |
| Edinburgh Self Storage Limited | Transport & logistics | 2005 |
| Edinburgh Self Storage Solutions Limited | Extraterritorial | 2017 |
| Edinburgh Storage Limited | Extraterritorial | 2017 |
| Edinburghweavingmill Ltd | Wholesale & retail | 2021 |
| Edinmech Limited | Manufacturing | 2017 |
| EGL Transport Limited | Transport & logistics | 2023 |
| Eight Property Letting Limited | Real estate | 2025 |
| Elan Consultancy Ltd | Professional & scientific | 2022 |
| Elder and Rowan Limited | Agriculture & fishing | 2022 |
| Electric Theatre Workshop Ltd | Arts & recreation | 2012 |
| Electrical Instrumentation Commissioning Services Limited | Construction | 2001 |
| ELEV8 Athletics Limited | Arts & recreation | 2023 |
| Elite (DFS) Ltd | Hospitality | 2022 |
| Elite Food House Limited | Wholesale & retail | 2024 |
| Elite Health Group Ltd | Health & social care | 2017 |
| Elite Taxi and Private Hire Ltd | Transport & logistics | 2017 |
| Elitestack Solutions Ltd | Information & communication | 2026 |
| Elsrickle Community Hub | Other services | 2024 |
| Elves CIC | Education | 2024 |
| Emac Engineering & Building Services Limited | Construction | 2013 |
| Emfields Solutions Ltd | Manufacturing | 2014 |
| Emmcha Properties Limited | Real estate | 2012 |
| Enable Driving Instructors Limited | Education | 2023 |
| Enable Driving School Limited | Education | 2024 |
| Energy Acres Ltd | Administrative & support | 2019 |
| Energy Centre Scotland Limited | Construction | 2011 |
| Epic Minds Limited | Real estate | 1979 |
| Essential Spirit Limited | Manufacturing | 2000 |
| EU Marketing Solutions Limited | Information & communication | 2025 |
| Euro Healthcare Services Limited | Wholesale & retail | 1993 |
| Eurocare Impex Services Limited | Wholesale & retail | 1992 |
| Eurocorp International Ltd | Information & communication | 2024 |
| Euroroute Limited | Wholesale & retail | 1988 |
| Euroroute Recovery Scotland Limited | Transport & logistics | 2021 |
| Evafp Limited | Hospitality | 2025 |
| Evolution Fitness Castle Douglas Ltd | Arts & recreation | 2022 |
| F5 Tech Ltd | Information & communication | 2022 |
| Fahm Property Investment Ltd | Real estate | 2021 |
| Faircraft Limited | Arts & recreation | 2011 |
| Fairhill Services Ltd | Agriculture & fishing | 2019 |
| Falu Studios Ltd | Professional & scientific | 2018 |
| Farm Business Services Limited | Real estate | 2006 |
| Farming Partners Arbigland Limited | Agriculture & fishing | 2020 |
| Farming Partners Dalswinton LLP | | 2024 |
| Farming Partners Holdings LLP | | 2024 |
| Farming Partners NZ Limited | Agriculture & fishing | 2023 |
| Fast Compute Ltd | Information & communication | 2023 |
| Faulds Properties Ltd | Real estate | 2021 |
| FBS Scotland Limited | Professional & scientific | 2023 |
| Fencer's Foundry Ltd | Wholesale & retail | 2025 |
| Fibre TEC Scotland Limited | Information & communication | 2022 |
| Filmsco Ltd | Information & communication | 2026 |
| Findlay Contracts Limited | Administrative & support | 2006 |
| Fine Property Limited | Hospitality | 1991 |
| Finite Engineering Limited | Construction | 2009 |
| Finix Medical Limited | Health & social care | 2026 |
| Finnie Commercial Properties Limited | Real estate | 2023 |
| Finnie Heavy Haulage Limited | Transport & logistics | 2002 |
| Finnie Properties Limited | Real estate | 2019 |
| Finnie Storage & Handling Ltd | Transport & logistics | 2025 |
| Finnie Storage Solutions Limited | Transport & logistics | 2010 |
| Fiona Holmes Properties Ltd | Real estate | 2019 |
| Firm of 1 Netherlocharwoods Farm | | 1988 |
| Firth Agri Holdings Limited | Financial services | 2024 |
| Fishdale Limited | Professional & scientific | 1990 |
| Fizz FIX Mini Market Ltd | Wholesale & retail | 2025 |