| Contracks Ltd | Other services | 2020 |
| Cool & Easy Ltd | Construction | 2015 |
| Cool & Easy Renewables Limited | Construction | 2022 |
| Coopland Consultancy Ltd. | Information & communication | 2004 |
| Coorie Inn Properties Limited | Real estate | 2026 |
| Copper Ball Plumbing Services Limited | Construction | 2017 |
| Copperstone Properties Ltd | Real estate | 2024 |
| Coppside Holsteins Limited | Agriculture & fishing | 2026 |
| Corberry Print Ltd | Manufacturing | 2015 |
| Core Building Maintenance Ltd | Construction | 2020 |
| Corner Field Property Limited | Real estate | 2022 |
| Cornerstones Property Ltd | Financial services | 2024 |
| Corona Fire Security and Infrastructure Limited | Administrative & support | 2024 |
| Coronal Limited | Real estate | 2026 |
| Corvus Hair Limited | Other services | 2023 |
| Cotland and Company (Dumfries) | | 1991 |
| Cottage by the Sea Ltd | Hospitality | 2020 |
| Cousland Properties Limited | Professional & scientific | 1986 |
| Cowar Farming Company Limited Partnership | | 1993 |
| Crab POT Colvend Ltd | Hospitality | 2024 |
| Craigiebrook Ltd | Real estate | 2020 |
| Cramond Scientific Limited | Professional & scientific | 2025 |
| Cree Childcare Limited | Health & social care | 2024 |
| Creighton Contracts Ltd | Construction | 2017 |
| Crichton Carbon Centre | Professional & scientific | 2007 |
| Crichton Development Company Limited | Real estate | 1995 |
| Crichton Foundation | Professional & scientific | 2009 |
| Crichton Hall Hotel & Spa Ltd | Hospitality | 2019 |
| Criffel Ashton Transport Limited | Transport & logistics | 2020 |
| Criffel Bakeries Limited | Real estate | 1978 |
| Criffel Cleaning Services Limited | Administrative & support | 2011 |
| Crindil Company Limited | Real estate | 2004 |
| CRJ Construction & Joinery Limited | Construction | 2019 |
| Crochmore (Holdings) Limited | Agriculture & fishing | 2019 |
| Crofthead | Administrative & support | 2007 |
| Crossan Domestic Appliances Limited | Manufacturing | 2018 |
| Crossdykes Community Benefits Limited | Other services | 2021 |
| Crossdykes Community Services Limited | Professional & scientific | 2021 |
| Crossflags (Motors) Limited | Wholesale & retail | 2019 |
| Crossroads (Dumfries & Lower Nithsdale) Care Attendant Scheme | Health & social care | 1997 |
| Crossy's Groundworks Limited | Construction | 2020 |
| Crown Facilities & Management Ltd | Administrative & support | 2022 |
| CRT Rentals Limited | Real estate | 2025 |
| Cruickshanks Engineering Limited | Manufacturing | 2014 |
| CSL Property Management Limited | Real estate | 2021 |
| Csr-Studios Limited | Information & communication | 2013 |
| Culyer Safety Services Limited | Professional & scientific | 2007 |
| Cumbria Green Fuels Ltd | Wholesale & retail | 2017 |
| Curamcare North Limited | Health & social care | 2018 |
| Currie International Holdings Limited | Transport & logistics | 2000 |
| Curry 2 U Limited | Hospitality | 2018 |
| Currywala Restaurants Limited | Hospitality | 2026 |
| Curve Prime Limited | Arts & recreation | 2018 |
| Cvfriends Limited | Information & communication | 2018 |
| CWM Roofing Ltd | Construction | 2026 |
| D & D'S Cleaning and Remediation Ltd | Transport & logistics | 2025 |
| D & G Caravan Storage Limited | Transport & logistics | 2023 |
| D & G Leisure Ltd | Wholesale & retail | 2025 |
| D & R Dickie (Hapland) & Co | | 2000 |
| D & R Dickie (Inglestone) & Co | | 2000 |
| D a Autoparts Limited | Wholesale & retail | 2020 |
| D E Builders Limited | Construction | 2024 |
| D K Engineering & Construction Ltd | Manufacturing | 2014 |
| D Mccormick Engineering Ltd | Manufacturing | 2024 |
| D S Marchbank Ltd | Wholesale & retail | 2017 |
| D Stephen Professional Tree Work Limited | Agriculture & fishing | 2013 |
| D W Construction and Joinery Limited | Construction | 2004 |
| D W Drennan Limited | Construction | 2009 |
| D&G Construction and Joinery Limited | Construction | 2017 |
| D&G Storage Ltd | Transport & logistics | 2023 |
| D-Lux Arts CIC | Arts & recreation | 2016 |
| D.A.Roberts (the Knowe) & Co | | 1991 |
| D.P. Properties (Lockerbie) Limited | Real estate | 1996 |
| Daes Civils Limited | Construction | 2024 |
| Daily Dish Ltd | Manufacturing | 2025 |
| Dalgarnock Pigs Limited | Agriculture & fishing | 2017 |
| Dalgliesh Forestry Limited | Agriculture & fishing | 2026 |
| Dalling Wealth Management Limited | Financial services | 2018 |
| Dalrymple SAW Services Limited | Manufacturing | 2003 |
| Dalscone Farm FUN | Hospitality | 2018 |
| Dalswinton Farming Partners Limited | Agriculture & fishing | 2024 |
| Dalziel Plumbing & Heating Limited | Construction | 2018 |
| Danderdee Limited | Information & communication | 2016 |
| Danderdee Technologies Limited | Information & communication | 2020 |
| Dardarroch Farm Limited | Agriculture & fishing | 2026 |
| DAT Financial Planning Ltd | Financial services | 2020 |
| Data Modify Ltd | Wholesale & retail | 2024 |
| David a. J. Hughes Limited | Professional & scientific | 2008 |
| David Hardie Engineering Ltd. | Construction | 1996 |
| David K Faulds Ltd | Construction | 2008 |
| David Marchbank (Bakers) Limited | Manufacturing | 2010 |
| David Reid Limited | Manufacturing | 2005 |
| David Sheridan Builders Limited | Construction | 2005 |
| David W Martin & Co Limited | Wholesale & retail | 2004 |
| Dbcc Operating Co Ltd | Hospitality | 2017 |
| DC Dumfries Lochside Tavern Limited | Hospitality | 2023 |
| DC Farming | Agriculture & fishing | 2022 |
| DC Solutions (Dumfries) Ltd | Administrative & support | 2019 |
| Dcrf Limited | Arts & recreation | 2026 |
| Dealio Brother Ltd | Wholesale & retail | 2025 |