| Patterson Refrigeration Ltd | Transport & logistics | 2026 |
| Pegasus Jewellery Ltd | Wholesale & retail | 2018 |
| Penkiln Sawmill Company Limited | Manufacturing | 1984 |
| People Place Policy Ltd | Professional & scientific | 2022 |
| PER4M Strength & Conditioning Ltd | Arts & recreation | 2023 |
| Perceptive Psychology Ltd | Education | 2021 |
| Personal Tours of the UK Ltd | Administrative & support | 2015 |
| Pesm Limited | Hospitality | 2025 |
| Peter C Haining Bathrooms Limited | Construction | 2008 |
| PH Energy Systems Ltd | Construction | 2023 |
| Pharma Trade UK Ltd | Wholesale & retail | 2024 |
| Philip Rycroft Consulting Limited | Professional & scientific | 2019 |
| Phoenix Dumfries Limited | Hospitality | 2021 |
| Phoenix Secure Ltd | Administrative & support | 2023 |
| Picaso Barber Ltd | Other services | 2024 |
| Piccolo Dumfries Ltd | Hospitality | 2021 |
| Pimlott Property Ltd | Real estate | 2020 |
| Pink Kitchen Community Enterprise C.I.C. | Hospitality | 2021 |
| Pisces Learning Innovations Limited | Professional & scientific | 2013 |
| Pistoia Ltd | Hospitality | 2026 |
| Pixel Forge Media Ltd | Construction | 2026 |
| Pizza Maxx Ltd | Hospitality | 2026 |
| Plascow Energy Limited | Energy | 2017 |
| Plastic Technology Services Limited | Manufacturing | 1991 |
| Pleacairn Offshore Limited | Mining & quarrying | 2021 |
| Pleacairn Services Limited | Construction | 2021 |
| Pleuchie's Howff Ltd | Hospitality | 2018 |
| Pocket Rocket Investments Limited | Real estate | 2014 |
| Pole Hoseason Limited | Construction | 2013 |
| Polish Shop Dino Dumfries Ltd | Wholesale & retail | 2017 |
| Pollock & Mclean LLP | | 2024 |
| Polymer Extrusion Technologies (UK) Limited | Manufacturing | 2014 |
| Post Conflict Solutions Limited | Administrative & support | 2020 |
| Power and Civils Limited | Administrative & support | 2024 |
| Power Base Services Ltd | Energy | 2016 |
| Powerhand Limited | Professional & scientific | 2001 |
| Powermac Joinery & Construction Limited | Construction | 2011 |
| Powerworks Limited | Construction | 2016 |
| Powhillon Farm | | 2000 |
| Precision Fitness (Lockerbie) Ltd | Arts & recreation | 2021 |
| Premier Poultry Limited | Agriculture & fishing | 2021 |
| Premier Taxis (Dumfries) Limited | Transport & logistics | 2011 |
| Presence Media Limited | Professional & scientific | 2026 |
| Project Abbie Limited | Transport & logistics | 2018 |
| Project Nicola Limited | Professional & scientific | 2019 |
| Prontop Ltd | Hospitality | 2017 |
| Property Rentals Scotland Limited | Real estate | 2023 |
| PTS (Scotland) Limited | Manufacturing | 2004 |
| Purdie Electrical Limited | Construction | 2024 |
| Purdie Transport | | 1986 |
| Pure Alba Maji Limited | Information & communication | 2019 |
| Purple Plum Ltd | Hospitality | 2018 |
| Quay Design (Architectural) Ltd | Professional & scientific | 2020 |
| Queen of the South Football Club, Limited | Arts & recreation | 1922 |
| Quick and Pro Decorating Limited | Construction | 2022 |
| R & M Whiteford | | 1994 |
| R a Spence | | 2002 |
| R Brothers Car Wash Ltd | Administrative & support | 2019 |
| R Hollis Contracting Limited | Administrative & support | 2020 |
| R Johnstone & Sons Limited | Real estate | 2003 |
| R R M Firm Limited | Professional & scientific | 2014 |
| R Waugh and Sons Ltd | Transport & logistics | 2024 |
| R. Barbour & Sons. Limited | Wholesale & retail | 1918 |
| R. Davidson & Sons Limited | Transport & logistics | 2007 |
| R. Earsman Limited | Construction | 1978 |
| R. Miroli Limited | Hospitality | 2014 |
| R.C Adamson Ltd | Agriculture & fishing | 2020 |
| R.W.Payne Limited | Professional & scientific | 2020 |
| Rachel Little Learning Solutions Ltd | Professional & scientific | 2019 |
| Radisoft Ltd | Manufacturing | 2016 |
| Rainbow Home Education Events Limited | Education | 2025 |
| Rajov Ltd | Hospitality | 2018 |
| RAN Morgan Consultancy Limited | Real estate | 2019 |
| Rapid Batch Limited | Manufacturing | 2024 |
| Rasul Property Investments Ltd | Real estate | 2025 |
| RAW Meats and Treats Limited | Wholesale & retail | 2025 |
| RAW Milk Limited | Manufacturing | 2023 |
| Raymond and Gourlay Developments Limited | Construction | 2020 |
| RC Energy Group Ltd | Energy | 2025 |
| Rchambers Company Ltd | Real estate | 2019 |
| RD Capital Ventures Ltd | Financial services | 2026 |
| RD Taylor Plant Hire Limited | Construction | 2024 |
| RDB Energy Limited | Professional & scientific | 2021 |
| RDS Joinery Limited | Construction | 2011 |
| Reburmac Limited | Real estate | 2025 |
| Red Berries Ltd | Construction | 2021 |
| Red Road Commercials Limited | Wholesale & retail | 2024 |
| Red Shoe Music Agency Ltd | Professional & scientific | 2022 |
| Red Star Lining Limited | Real estate | 2025 |
| Redman Compliance Services Limited | Professional & scientific | 2023 |
| Refresh Aesthetics Clinic Ltd | Other services | 2026 |
| Regent Property Investments & Developments Ltd | Real estate | 2010 |
| Reid Pipeline Construction Limited | Construction | 2010 |
| Reliable Automotive Ltd | Wholesale & retail | 2022 |
| REM Homes Limited | Real estate | 2018 |
| Rerrick Contract Services Limited | Agriculture & fishing | 2012 |
| Rescyou Ltd | Education | 2025 |
| Reset & Rebalance Limited | Other services | 2026 |
| Resonant Press Ltd | Information & communication | 2026 |
| Retro Productions Limited | Arts & recreation | 2019 |