| Scott Allan Limited | Wholesale & retail | 2022 |
| Sculpt Aesthetics Yorkshire Limited | Other services | 2022 |
| Sculpt Studio Limited | Other services | 2024 |
| SD Location Catering Limited | Hospitality | 2019 |
| Select Silverware Ltd | Manufacturing | 2003 |
| Seong Barbers Ltd | Other services | 2024 |
| Seong Styles Ltd | Other services | 2026 |
| Serin Property Ltd | Real estate | 2023 |
| Severn Architects Ltd | Professional & scientific | 2020 |
| SFK Advisory Ltd | Professional & scientific | 2026 |
| Sharples and SON Electrical Limited | Energy | 2015 |
| Shaun Boyd Engineering Limited | Professional & scientific | 2014 |
| Shaw Developments (Sheffield) Limited | Construction | 1986 |
| Shaws 4 Paws Limited | Wholesale & retail | 2026 |
| Sheff Sports Performance Ltd | Wholesale & retail | 2024 |
| Sheffield F.C. the Oldest Football Club in the World' EST 1857 Ltd. | Arts & recreation | 1995 |
| Sheffield Fracture Mechanics Limited | Professional & scientific | 2025 |
| Sherburns LGV Ltd | Education | 2015 |
| Shires Developments (Holdings) Limited | Mining & quarrying | 2007 |
| Shires Developments Limited | Mining & quarrying | 1995 |
| Sidcol Limited | Construction | 2002 |
| Sidey Electrical Limited | Construction | 2021 |
| Silkcraft Limited | Wholesale & retail | 2006 |
| Simply Sorted Surrey Ltd | Administrative & support | 2025 |
| Singleton & Singleton Ltd | Professional & scientific | 2026 |
| Sirius Window Films Ltd | Wholesale & retail | 2026 |
| Sivil Developments Limited | Construction | 2016 |
| Sivil Enterprises Limited | Construction | 2013 |
| Sivil Invest Limited | Construction | 2016 |
| Sixcore Express Ltd | Transport & logistics | 2025 |
| Skeltonhall Limited | Extraterritorial | 2017 |
| SKI and Sport Limited | Administrative & support | 2008 |
| Sky High Bungee Limited | Arts & recreation | 2002 |
| Smart Risk & Compliance Ltd | Professional & scientific | 2026 |
| Smarter Mortgages Ltd | Financial services | 2019 |
| Smilo Cover Limited | Administrative & support | 2025 |
| Soft Options Interiors Limited | Wholesale & retail | 2011 |
| Soil Conditioners Ltd | Extraterritorial | 2006 |
| Some FUN 4X the Weekend Limited | Wholesale & retail | 2025 |
| Sommer Bear Limited | Professional & scientific | 2020 |
| Sorono Innovations Ltd | Construction | 2010 |
| South Yorkshire Business Consulting Ltd | Professional & scientific | 2025 |
| Spencer Sheridan Limited | Administrative & support | 2011 |
| Spin and Thread Limited | Wholesale & retail | 2024 |
| Spiv Tech Ltd | Manufacturing | 2024 |
| Spring Care for YOU Limited | Health & social care | 2024 |
| SRK Pneumatics & Engineering Ltd | Manufacturing | 2002 |
| ST Andrews Community Kids Club CIC | Health & social care | 2025 |
| ST Enodoc Holdings Limited | Hospitality | 2024 |
| ST Enodoc Hotel Limited | Hospitality | 2018 |
| Stagden Slaney Ltd | Professional & scientific | 2010 |
| Stagg Mortgage Services Limited | Financial services | 2023 |
| Star Precision Limited | Professional & scientific | 2011 |
| Staves Estate Agents of Dronfield Limited | Real estate | 2015 |
| Stay Warm Windows Limited | Construction | 2017 |
| Steamtank Ltd | Education | 2024 |
| Steel City Living Limited | Real estate | 2017 |
| Steel City Sports and Prestige Limited | Extraterritorial | 2018 |
| Stem Media Limited | Information & communication | 2022 |
| Stratton Oakmount Holdings Ltd | Administrative & support | 2023 |
| Stubley Christmas Tree Farm Ltd | Agriculture & fishing | 2026 |
| Stubley Ltd | Health & social care | 2024 |
| Stubley Medical LLP | | 2007 |
| Stubley Property Management Ltd | Real estate | 2024 |
| Student LED UNI SKI Holidays Ltd | Administrative & support | 2025 |
| Studio Silent Ltd | Information & communication | 2020 |
| Success Alchemy Limited | Education | 2021 |
| Sulvan Limited | Real estate | 2024 |
| Summerley Smile Design Ltd | Health & social care | 2025 |
| Sunflower Compliance Ltd | Professional & scientific | 2022 |
| Sunny Minds Ltd | Education | 2020 |
| Supafit Limited | Manufacturing | 1999 |
| Supply and Demand Electrical Services Ltd. | Administrative & support | 2017 |
| Supreme Auctions Limited | Wholesale & retail | 2020 |
| Supreme Property Holdings Limited | Real estate | 2023 |
| Supreme Protective Coatings Limited | Wholesale & retail | 2003 |
| Supremecoat Group Ltd | Extraterritorial | 2022 |
| Supremecoat Health Ltd | Wholesale & retail | 2020 |
| Supremecoat International Ltd | Wholesale & retail | 2020 |
| Supremecoat Ltd | Wholesale & retail | 2020 |
| Surgecore Limited | Information & communication | 2025 |
| Sustainingability Ltd | Professional & scientific | 2023 |
| Swift Business Improvement Limited | Professional & scientific | 2014 |
| SXI Coaching Academy Ltd | Arts & recreation | 2025 |
| T Hanson Property Limited | Real estate | 2026 |
| T S Contracting Ltd | Administrative & support | 2026 |
| Talbot Motor Co Ltd | Wholesale & retail | 2018 |
| Tanning & Beauty Spa Limited | Other services | 2024 |
| Tasker's Engineering Co. Limited | Manufacturing | 2014 |
| Tatras Residential Limited | Real estate | 2021 |
| Taylors (Broomhill) Limited | Transport & logistics | 2007 |
| TDO Properties Ltd | Real estate | 2021 |
| Tech Ware One Limited | Information & communication | 2014 |
| Technative Solutions Ltd | Information & communication | 2017 |
| Technotech Ltd | Information & communication | 2018 |
| TECTUM1 Ltd | Other services | 2024 |
| TEDDY4LIFE Limited | Wholesale & retail | 2019 |
| Tela Electrical Ltd | Construction | 2023 |
| Telwyn Limited | Professional & scientific | 2013 |
| Temper Brewing Company Ltd | Manufacturing | 2021 |